不同遗传背景猪群体黏病毒耐药蛋白1基因多态性分析

doi:10.16431/j.cnki.1671-7236.2017.04.016

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (4): 1046-1053.doi: 10.16431/j.cnki.1671-7236.2017.04.016

不同遗传背景猪群体黏病毒耐药蛋白1基因多态性分析

张立春¹, 李兆华¹, 李梦姝^1,2, 孙福亮², 曹阳¹, 朴庆林¹, 金海国¹, 张树敏¹

1. 吉林省农业科学院畜牧分院, 公主岭 136100;
2. 延边大学农学院, 延吉 133002

收稿日期:2016-10-08 出版日期:2017-04-20 发布日期:2017-05-03
通讯作者: 张树敏 E-mail:shumin1961@126.com
作者简介:张立春(1977-),男,吉林榆树人,博士,副研究员,研究方向:动物生物技术,E-mail:zhang_lich@163.com
基金资助:
吉林省重点科技公关项目（20140204061NY）

The Polymorphism Analysis of Mx1 gene in Pig Populations from Different Genetic Background

ZHANG Li-chun¹, LI Zhao-hua¹, LI Meng-shu^1,2, SUN Fu-liang², CAO Yang¹, PIAO Qing-lin¹, JIN Hai-guo¹, ZHANG Shu-min¹

1. Branch of Animal Husbandry, Jilin Academy of Agricultural Science, Gongzhuling 136100, China;
2. Agricultural College of Yanbian University, Yanji 133002, China

Received:2016-10-08 Online:2017-04-20 Published:2017-05-03

摘要/Abstract

摘要：

为探讨不同遗传背景猪黏病毒耐药蛋白1（myxovirus resistance，Mx1）基因的遗传多态性，本试验采用RT-PCR方法扩增克隆出猪Mx1基因并进行生物信息学分析，采用高分辨率熔解曲线法（HRM）对民猪、长白山野猪与民猪杂交猪（简称野杂猪）和大白猪3个群体Mx1基因外显子2区多态性进行分析。结果显示，从民猪及野杂猪中成功克隆出4条Mx1基因mRNA序列。该序列含有1个1 992 bp的完整开放阅读框（ORF），编码663个氨基酸。比较发现4条Mx1基因序列中共含有30个SNPs位点，其中17个SNPs位点引起了氨基酸序列的变异。功能结构域分析发现，大部分氨基酸突变位点位于Mx1基因功能结构域两侧。HRM多态性分析显示，Mx1基因外显子2区DD基因型作为优势基因型在3个群体中具有最高的基因型频率，其中大白猪中DD基因型频率为100%，野杂猪为62.5%，民猪为56.2%。基因多态性分析还发现该区域存在更多的核苷酸及氨基酸突变位点，表明民猪和野杂猪Mx1基因存在更丰富的基因多态性。

关键词: 黏病毒耐药蛋白1基因; 民猪; 大白猪; 野杂猪; 基因多态性

Abstract:

In order to explore the polymorphisms of myxovirus resistance (Mx1) gene in pig populations from different genetic background,we cloned Mx1 gene and tested the polymorphisms on exon 2 of Mx1 gene in Min pig, crossbred from wild boars in Changbai Mountain with Min pig (crossbred wild boars) and Large White pig population using high resolution melting (HRM). The results showed that four Mx1 mRNAs were cloned from Min pig and crossbred wild boars, and they had whole ORF which was 1 992 bp in length and coded 663 amino acids. In the four Mx1 mRNA, there were 30 SNPs in nucleotide and 17 mutations in amino acid. The location of SNPs displayed that almost mutations located on two sides of the function domains in Mx1 protein. The polymorphism data in HRM test showed that the DD genotype was the dominant genotype which had the highest frequency in three populations. The DD genotype frequency was 100% in Large White pig population, 62.5% in crossbred wild boars, and 56.2% in Min pig.Some novel mutations in both nucleotide and amino acid on exon 2 region were explored in polymorphism test. In conclusion, there was more polymorphism of Mx1 gene in Min pig and crossbred wild boars.

Key words: Mx1 gene; Min pig; Large White pig; crossbred wild boars; gene polymorphism

中图分类号:

S813.3

张立春, 李兆华, 李梦姝, 孙福亮, 曹阳, 朴庆林, 金海国, 张树敏. 不同遗传背景猪群体黏病毒耐药蛋白1基因多态性分析[J]. 《中国畜牧兽医》, 2017, 44(4): 1046-1053.

ZHANG Li-chun, LI Zhao-hua, LI Meng-shu, SUN Fu-liang, CAO Yang, PIAO Qing-lin, JIN Hai-guo, ZHANG Shu-min. The Polymorphism Analysis of Mx1 gene in Pig Populations from Different Genetic Background[J]. , 2017, 44(4): 1046-1053.

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不同遗传背景猪群体黏病毒耐药蛋白1基因多态性分析

The Polymorphism Analysis of Mx1 gene in Pig Populations from Different Genetic Background

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