›› 2011, Vol. 38 ›› Issue (7): 159-162.

• 遗传繁育 • 上一篇    下一篇

洼地绵羊FecB基因多态性与其产羔数关系的研究

任艳玲1,2, 沈志强1,2, 李敏3, 肖娜3, 董文艳3, 李金林1,2, 王建军1,2   

  1. 1. 山东省滨州畜牧兽医研究院,山东滨州 256600;2. 山东省洼地绵羊繁育技术研究推广中心,山东滨州 256600;3. 山东绿都生物科技有限公司,山东滨州 256600
  • 收稿日期:2010-12-14 修回日期:1900-01-01 出版日期:2011-07-20 发布日期:2011-07-20
  • 通讯作者: 沈志强

Association of Polymorphisms in the FecB Gene with Litter Size in Wadi Sheep

REN Yan-ling1,2, SHEN Zhi-qiang1,2, LI Min3, XIAO Na3, DONG Wen-yan3, LI Jin-lin1,2, WANG Jian-jun1,2   

  1. 1. Shandong Binzhou Anmimal Science & Veterinary Medicine Institute,Binzhou 256600,China;2. Research and Development Center of Wadi Sheep Breeding Technology,Binzhou 256600,China;3. Shandong Lvdu Biotechnology Ltd., Binzhou 256600,China
  • Received:2010-12-14 Revised:1900-01-01 Online:2011-07-20 Published:2011-07-20

摘要: 为了从分子水平上研究洼地绵羊的多羔机制,本研究采用PCR-RFLP方法分析洼地绵羊中FecB基因多态性及其与产羔数的关系。结果表明,洼地绵羊中存在与Booroola羊相同的FecB基因突变,有B/B、B/+、+/+ 3种基因型,其基因型频率分别为0.29、0.56和0.15;且4乳头个体FecB基因频率高于2乳头个体;3种基因型的平均产羔数分别为2.81±0.42、2.49±0.53和1.96±0.28只。经统计分析,B/B与B/+基因型洼地绵羊的产羔数显著高于+/+基因型(P<0.05),B/B与B/+基因型之间差异不显著(P>0.05),而+/+基因型个体也存在多羔现象。结果提示,FecB基因可能是洼地绵羊多羔性能主效基因之一,但洼地绵羊可能还存在其它影响多羔性能的基因。

关键词: 洼地绵羊; FecB基因; 多态性; 产羔数

Abstract: To study the fecundity of Wadi sheep on molecular level,polymorphisms within the FecB gene of Wadi sheep and associations with litter size were analyzed by PCR-RFLP. The results showed that there was FecB mutation in Wadi sheep that was the same as in Booroola,and the frequencies of three genotypes (B/B,B/+,+/+) were 0.29,0.56 and 0.15,respectively. An association between genotype frequencies and number of nipples was observed. Average litter sizes for the three genotypes were 2.81±0.42,2.49±0.53 and 1.96±0.28,respectively. Statistical analysis showed that litter size of Wadi sheep with B/B and B/+ genotypes were significantly greater than that of sheep with the +/+ genotypes (P<0.05),but differences between the B/B and B/+ genotypes were not significant(P>0.05). These results indicated that the FecB gene could be of great interest in the Wadi sheep breed due to its association with fecundity.

Key words: Wadi sheep; FecB gene; polymorphism; litter size

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