›› 2010, Vol. 37 ›› Issue (3): 145-147.

• 遗传繁育 • 上一篇    下一篇

豫南黑猪FSHβ亚基基因多态性研究

冯富彦,李新建,吕刚,张弛,任广志   

  1. (河南农业大学牧医工程学院, 郑州 450002)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-03-20 发布日期:2010-03-20
  • 通讯作者: 任广志

Polymorphism Analysis of FSHβ Subunit Gene in Yunan Black Pigs

FENG Fu-yan,LI Xin-jian,LV Gang,ZHANG Chi,REN Guang-zhi   

  1. (College of Animal Husbandry and Veterinary,Henan Agricultural University,Zhengzhou 450002,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-03-20 Published:2010-03-20

摘要: 采用聚合酶链式反应(polymerase chain reaction,PCR)技术对豫南黑猪FSHβ亚基基因扩增后进行多态性分析,并与文献报道的国内外猪种进行比较。结果显示,豫南黑猪猪群中FSHβ亚基基因的A、B基因频率分别为0.3318、0.6682;基因型AA、AB、BB分别为0.0727、0.5182、0.4091,基因型分布符合哈迪—温伯格平衡。群体多态信息含量(PIC)为0.3451,属于中度多态;杂合度为0.4434,显示具有较大的选择潜力。聚类分析结果显示,豫南黑猪与民猪的亲缘关系最近,与二花脸猪的亲缘关系相对较远。

关键词: 豫南黑猪; FSHβ亚基基因; 多态性; 遗传距离; 聚类分析

Abstract: The FSHβ subunit gene was obtained using the polymerase chain reaction(PCR) technique in Yunan black pigs,and the polymorphism was investigated. The results showed that the allele frequencies of A,B were 0.3318 and 0.6682. The genotype frequencies of AA,AB,BB were 0.0727,0.5182 and 0.4091, the genotype frequencies of the three genes were in Hardy-Weinberg equilibrium.The polymorphism information content (PIC) was 0.3451,belong to midpolymorphism locus,and heterozygosity was 0.4434, showed that Yunan black pigs had a good potential for breeding.The cluster analysis suggested that the relationship was closer between Yunan black pigs and Min pigs,however,the relationship was relatively farther between Yunan black pigs and Erhualian pigs.

Key words: Yunan black pigs; FSHβ subunit gene; polymorphism; genetic distance; cluster analysis

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