英纯血马蹄病与血液指标和肠道菌群组成的相关性研究

doi:10.16431/j.cnki.1671-7236.2024.05.040

摘要/Abstract

摘要： 【目的】研究马患蹄病与血液指标和肠道微生物菌群组成之间的关系。【方法】根据临床检查挑选6匹(12±3)岁龄患蹄病的英国纯血母马为试验组(S组),另选6匹体重、年龄相近的英纯血健康母马作为对照组(C组),对试验马进行3 d的临床检查及评估。采集两组马颈静脉血及直肠粪便,进行血常规、血液生化指标检测;采用16S rRNA测序法检测马肠道菌群的组成。【结果】临床检查发现,S组马均有跛行,蹄甲变形等症状。血常规检测结果显示,两组马血液中各指标均差异不显著(P＞0.05),其中S组马血液中白细胞数和中性粒细胞数均高于正常范围,血红蛋白浓度低于正常范围。血液生化指标检测结果显示,与C组相比,S组马血液中甘油三酯、总蛋白、尿素、肌酐含量等呈现上升趋势,白蛋白、总胆红素等指标呈现下降趋势(P>0.05)。16S rRNA测序结果显示,在门分类水平上,与C组相比,S组马肠道菌群中迷踪菌门(Elusimicrobia)相对丰度显著降低(P＜0.05),SR1菌相对丰度极显著降低(P＜0.01);在属分类水平上,与C组相比,S组马肠道菌群中魏斯氏菌属(Weissella)、土微菌属(Pedomicrobium)、海洋杆菌属(Pontibacter)、库特氏菌属(Kurthia)相对丰度显著增加(P＜0.05),埃希氏菌属(Escherichia)、韧皮部杆菌属(Candidatus_Endomicrobium)、微小杆菌属(Microvirga)、消化球菌属(Peptococcus)、普雷沃氏菌属(Prevotella)相对丰度显著降低(P＜0.05)。两组马肠道菌群之间存在显著差异的菌种共10种。【结论】马发生蹄病后体内会出现炎症反应,造成肝肾功能受到损伤、肠道菌群物种丰度发生改变,同时消化能力降低。研究结果为进一步探索肠道菌群-代谢紊乱与马蹄病的关系奠定了基础,为通过调控肠道菌群防治马蹄病的发生提供了线索。

关键词: 英纯血马; 蹄病; 血液指标; 肠道菌群

Abstract: 【Objective】 This study was conducted to investigate the relationship between hoof disease and blood indicators and intestinal microbiota composition.【Method】 According to clinical examination,6 British thoroughbred mares aged (12±3) years old with hoof disease were selected as the experimental group (group S),and 6 healthy British thoroughbred mares with similar weight and age were selected as the control group (group C).Clinical examination and evaluation were performed for 3 days.The cervical venous blood and rectal feces of the two groups were collected,and blood routine and blood biochemical examination were performed.16S rRNA sequencing was used to detect the composition of intestinal flora in horses.【Result】 Clinical examination showed that horses in group S had lameness,hoof nail deformation and other symptoms.Blood routine test showed that there were no significant differences in blood indexes between the two groups (P＞0.05), among which the leukocyte count and neutrophil count in the blood of group S were higher than the normal range, and the hemoglobin concentration was lower than the normal range.Blood biochemical tests showed that compared with group C,the contents of triglyceride,total protein,urea,creatinine in group S showed an upward trend,while the contents of albumin,serum total bilirubin and other indicators showed a downward trend (P>0.05).16S rRNA sequencing results showed that at the phylum level,compared with group C,the relative abundance of Elusimicrobia in group S was significantly decreased (P＜0.05),and relative abundance of SR1 was extremely significantly decreased (P＜0.01).At the genus level,compared with group C,the relative abundance of Weissella,Pedomicrobium,Pontibacter and Kurthia in group S was significantly increased (P＜0.05),and the relative abundance of Escherichia,Candidatus_Endomicrobium,Microvirga,Peptococcus and Prevotella were significantly decreased (P＜0.05).There were 10 bacteria with significant difference in intestinal flora between the two groups. 【Conclusion】 After the occurrence of hoof disease,there would be inflammation in the body,resulting in damage to liver and kidney function,changes in the abundance of intestinal flora,and reduced digestive capacity.The results laid a foundation for further exploration of the relationship between intestinal flora and metabolic disorders and hoof disease,and provided clues for the prevention and treatment of hoof disease by regulating intestinal flora.

Key words: British thoroughbred horses; hoof disease; blood indexes; intestinal flora

中图分类号:

S858.21

卢冲, 卢亚宾, 付涵, 缪荣浩, 买占海, 况玲. 英纯血马蹄病与血液指标和肠道菌群组成的相关性研究[J]. 中国畜牧兽医, 2024, 51(5): 2198-2209.

LU Chong, LU Yabin, FU Han, MIAO Ronghao, MAI Zhanhai, KUANG Ling. Study on the Correlation Between Hoof Disease and Blood Indexes and Posterior Intestinal Flora in British Thoroughbred Horses[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(5): 2198-2209.

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