有机酸对猪肠道菌群的调节作用及其抑菌机制研究进展

doi:10.16431/j.cnki.1671-7236.2023.08.011

摘要/Abstract

摘要： 肠道菌群平衡与动物机体健康及生长发育密切相关,有机酸(organic acid,OA)作为一种绿色安全的功能性饲料添加剂,对动物具有抑菌、促消化、调节菌群平衡等作用。随着饲料产业的不断发展,OA已从饲料酸化、防腐、防霉逐渐向制剂化、动物生长促进剂和替抗产品转变,已成为畜牧行业广泛使用的一种功能性添加剂。笔者综述了猪肠道菌群的形成和分布,OA调节猪肠道菌群的组成结构和多样性及其对肠道菌群代谢产物产生和肠道免疫反应等方面的影响,并对其调控猪肠道菌群的作用机制进行探讨,旨在进一步揭示OA与肠道菌群的相互影响机制,为促进猪肠道健康与OA的合理利用提供借鉴。

关键词: 猪; 有机酸; 肠道菌群; 微生物

Abstract: The balance of intestinal flora is closely related to the health and growth of animals.As a green and safe functional feed additive,organic acid (OA) has the effects of bacteriostasis,promoting digestion and regulating flora balance.With the continuous development of feed industry,OA has been gradually transformed from feed acidification,anticorrosion and mildew prevention to preparation,animal growth enhancers and anti-resistant products,and has become a functional additive widely used in the animal husbandry industry.The author reviewed the formation and distribution of pig intestinal flora,the effects of OA on the composition and diversity of pig intestinal flora,the production of metabolites and intestinal immune response,and the mechanism of regulating pig intestinal flora was discussed in order to further reveal the interaction mechanism between OA and intestinal flora,and provide reference for promoting pig intestinal health and rational utilization of OA.

Key words: pigs; organic acid; intestinal flora; microorganisms

中图分类号:

S831.5

文伟, 胡友军, 程皇座, 何军. 有机酸对猪肠道菌群的调节作用及其抑菌机制研究进展[J]. 中国畜牧兽医, 2023, 50(8): 3133-3141.

WEN Wei, HU Youjun, CHENG Huangzuo, HE Jun. Research Progress of Regulating Effect of Organic Acids on Intestinal Flora of Pigs and Its Bacteriostatic Mechanism[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(8): 3133-3141.

参考文献

[1] DOOD A J,FIELDS K B,RAKER J R.Using lexical analysis to predict lewis acid-base model use in responses to an acid-base proton-transfer reaction[J].Journal of Chemical Education,2018,95(8):1267-1275.
[2] MURPHY P V.Lewis acid promoted anomerisation:Recent developments and applications[J].Carbohydrate Chemistry,2015,2:90-123.
[3] COLE D,BEAL R,LUSCOMBE J.The effect on performance and bacetrial flora of lactic acid,propionic acid,calcium propionate and calcium acrylate in the drinking water of the weaned pigs[J].Veterinary Record, 2020,10(9):1608.
[4] PAPATSIROS V G,BILLINIS C.The prophylactic use of acidifiers as antibacterial agents in swine[J].Antimicrobial Agents,2012,295:310.
[5] DAHMER P L,JONES C K.Evaluating dietary acidifiers as alternatives for conventional feed-based antibiotics in nursery pig diets[J].Translational Animal Science,2021,5(2):40.
[6] RON S,SHAI F,RON M.Revised estimates for the number of human and bacteria cells in the body[J].PLoS Biology,2016,14(8):e1002533.
[7] JABER A M,JONNA J,TIINA P M,et al.The composition of the perinatal intestinal microbiota in cattle[J].Scientific Reports,2018,8(1):10437.
[8] KARSTRUP C C,KLITGAARD K,JENSEN T K,et al.Presence of bacteria in the endometrium and placentomes of pregnant cows[J].Theriogenology,2017,99:41-47.
[9] TIAN L,BRUGGEMAN G,VAN DEN BERG M,et al.Effects of pectin on fermentation characteristics,carbohydrate utilization,and microbial community composition in the gastrointestinal tract of weaning pigs[J].Molecular Nutrition&Food Research,2017,61(1):1600186.
[10] LI M,LONG S,WANG Q,et al.Mixed organic acids improve nutrients digestibility,volatile fatty acids composition and intestinal microbiota in growing-finishing pigs fed high-fiber diet[J]. Asian-Australasian Journal of Animal Sciences,2019,32(6):856-864.
[11] 李浩,谷雪玲,吕宏伟,等.仔猪肠道菌群的母源传递[J].动物营养学报,2019,31(9):3983-3990. LI H,GU X L,LYU H W,et al.Maternal transmission of intestinal microflora in piglets[J].Chinese Journal of Animal Nutrition, 2019,31(9):3983-3990.(in Chinese)
[12] LEE S J,KIM J Y,PARK N H,et al.Effect of dietary fiber source on the growth performance and intestinal microflora in piglets[J].International Journal of Applied Research in Veterinary Medicine,2016,14(2):135-142.
[13] SLIFIERZ M J,FRIENDSHIP R M,WEESE J S.Longitudinal study of the early-life fecal and nasal microbiotas of the domestic pig[J].BMC Microbiology,2015,15(1):1-12.
[14] LONG S F,XU Y T,PAN L,et al.Mixed organic acids as antibiotic substitutes improve performance,serum immunity,intestinal morphology and microbiota for weaned piglets[J]. Animal Feed Science and Technology,2018,235:23-32.
[15] UPADHAYA S D,LEE K Y,KIM I H.Influence of protected organic acid blends and diets with different nutrient densities on growth performance,nutrient digestibility and faecal noxious gas emission in growing pigs[J].Veterinarine Medicina,2014,59(10):491-497.
[16] AHMED S T,HWANG J A,HOON J,et al.Comparison of single and blend acidifiers as alternative to antibiotics on growth performance,fecal microflora,and humoral immunity in weaned piglets[J].Asian-Australasian Journal of Animal Sciences,2014,27(1):93-100.
[17] KUANG Y,WANG Y,ZHANG Y,et al.Effects of dietary combinations of organic acids and medium chain fatty acids as a replacement of zinc oxide on growth,digestibility and immunity of weaned pigs[J].Animal Feed Science and Technology,2015,208:145-157.
[18] DOU S,GADONNA-WIDEHEM P,ROME V,et al.Characterisation of early-life fecal microbiota in susceptible and healthy pigs to post-weaning diarrhoea[J].PLoS One,2017,12(1):e0169851.
[19] HANCZAKOWSKA E,WITKIEWICZ M,NATONEK-WINIEWSKA M,et al.Medium chain fatty acids (MCFA) and/or probiotic Enterococcus faecium as a feed supplement for piglets[J].Livestock Science,2016,192:1-7.
[20] OMAR N A M,WU H,LARSSON A,et al.Long-term whole-grain rye and wheat consumption and their associations with selected biomarkers of inflammation,endothelial function,and cardiovascular disease[J].European Journal of Clinical Nutrition,2021,75(1):123-132.
[21] DIAO H,ZHENG P,YU B,et al.Effects of dietary supplementation with benzoic acid on intestinal morphological structure and microflora in weaned piglets[J].Livestock Science,2014,167:249-256.
[22] YU L,ZHANG S,XU Y,et al.Acid resistance of E.coli O157:H7 and O26:H11 exposure to lactic acid revealed by transcriptomic analysis[J].LWT-Food Science and Technology,2021,136:110352.
[23] ZHANG F,MA T,CUI P,et al.Diversity of the gut microbiota in dihydrotestosterone-induced pcos rats and the pharmacologic effects of diane-35,probiotics,and berberine[J].Frontiers in Microbiology,2019,10:175.
[24] MUDARRA R A,CHENG T,KRISTOPHER B,et al.Effect of adding bioactive peptide in combination of pharmaceutical zinc oxide or organic acids on growth performance,hematology profile,and nutrient digestibility in nursery pigs[J].Journal of Animal Science,2021,99(91):22-23.
[25] XU Y,LAHAYE L,HE Z,et al.Micro-encapsulated essential oils and organic acids combination improves intestinal barrier function,inflammatory responses and microbiota of weaned piglets challenged with enterotoxigenic Escherichia coli F4(K88+)[J].Animal Nutrition,2020,6(3):269-277.
[26] KNARREBORG A,MIQUEL N,GRANLI T,et al.Establishment and application of an in vitro methodology to study the effects of organic acids on coliform and lactic acid bacteria in the proximal part of the gastrointestinal tract of piglets[J].Animal Feed Science&Technology,2002,99(1-4):131-140.
[27] GUEVARRA R B,HONG S H,CHO J H,et al.The dynamics of the piglet gut microbiome during the weaning transition in association with health and nutrition[J].Journal of Animal Science and Biotechnology,2018,9(4):971-979.
[28] DEVI S M,LEE K Y,KIM I H.Analysis of the effect of dietary protected organic acid blend on lactating sows and their piglets[J].Revista Brasileira de Zootecnia,2016,45:39-47.
[29] LI S,ZHENG J,DENG K,et al.Supplementation with organic acids showing different effects on growth performance,gut morphology and microbiota of weaned pigs fed with highly or less digestible diets[J].Journal of Animal Science,2018,8(11):3302-3318.
[30] HANCZAKOWSKA E,AGNIESZKA S,KRZYSZTOF O.Effects of dietary caprylic and capric acids on piglet performance and mucosal epithelium structure of the ileum[J].Journal of Animal&Feed Sciences,2011,20(4):556-565.
[31] LUISE D,MOTTA V,SALVARANI C,et al.Long-term administration of formic acid to weaners:Influence on intestinal microbiota,immunity parameters and growth performance[J].Animal Feed Science&Technology,2017,232:160-168.
[32] UPADHAYA S D,LEE K Y,SERPUNJA S,et al.Growth performance,nutrient digestibility,fecal microbiota and fecal noxious gas emission in weaning pigs fed high and low density diet with and without protected organic acid blends[J].Animal Feed Science and Technology,2018,239:1-8.
[33] YANG Y,LEE K Y,KIM I H.Effects of dietary protected organic acids on growth performance,nutrient digestibility,fecal microflora,diarrhea score,and fecal gas emission in weanling pigs[J].Canadian Journal of Animal Science,2019,3:99.
[34] ZOU X,JI J,QU H,et al.Effects of sodium butyrate on intestinal health and gut microbiota composition during intestinal inflammation progression in broilers[J]. Poultry Science,2019,98(10):4449-4456.
[35] LAN R,KIM I.Effects of organic acid and medium chain fatty acid blends on the performance of sows and their piglets[J]. Animal Science Journal,2018,89(12):1673-1679.
[36] ZENTEK J,FERRARA F,PIEPER R,et al.Effects of dietary combinations of organic acids and medium chain fatty acids on the gastrointestinal microbial ecology and bacterial metabolites in the digestive tract of weaning piglets[J].Journal of Animal Science,2013,91(7):3200-3210.
[37] KOH A,DE VADDER F,KOVATCHEVA-DATCHARY P,et al.From dietary fiber to host physiology:Short-chain fatty acids as key bacterial metabolites[J].Cell,2016,165(6):1332-1345.
[38] KIM M H,KANG S G,PARK J H,et al.Short-chain fatty acids activate GPR41 and GPR43 on intestinal epithelial cells to promote inflammatory responses in mice[J].Gastroenterology,2013,145(2):396-406.
[39] SINGH N,GURAV A,SIVAPRAKASAM S,et al.Activation of Gpr109a,receptor for niacin and the commensal metabolite butyrate,suppresses colonic inflammation and carcinogenesis[J].Immunity,2014,40(1):128-139.
[40] KIM D,NGUYEN Q T,LEE J,et al.Anti-inflammatory roles of glucocorticoidsare mediated by Foxp3+regulatory T cells via a miR-342-dependent mechanism[J].Immunity,2020,53(3):581-596.
[41] LANDUYT A E,KLOCKE B J,COLVIN T B,et al.Cutting edge:ICOS-deficient regulatory T cells display normal induction of Il10 but readily downregulate expression of Foxp3[J].Journal of Immunology,2019,202(4):1039-1044.
[42] NUTSCH K,CHAI J N,AI T L,et al.Rapid and efficient generation of regulatory T cells to commensal antigens in the periphery[J].Cell Reports,2016,17(1):206-220.
[43] LUU M,MONNING H,VISEKRUNA A.Exploring the molecular mechanisms underlying the protective effects of microbial SCFAs on intestinal tolerance and food allergy[J].Frontiers in Immunology,2020,11:1225.
[44] AGATHA S,BRUHS A,SCHWARZ T.Commensal microbe-derived short-chain fatty acids regulate cutaneous immunity via IL-10 releasing regulatory T cells[J].Journal of Dermatological Science,2017,86(2):e66.
[45] BYNDLOSS M X,OLSAN E,RIVERA-CHÁVEZ F,et al.Microbiota-activated PPAR-γ signaling inhibits dysbiotic enterobacteriaceae expansion[J].Science,2017,357(6351):570-575.
[46] PARK J,KIM M,KANG S G,et al.Short-chain fatty acids induce both effector and regulatory T cells by suppression of histone deacetylases and regulation of the mTOR-S6K pathway[J]. Mucosal Immunology,2015,8(1):80-93.
[47] OH J H,ALEXANDER L M,PAN M,et al.Dietary fructose and microbiota-derived short-chain fatty acids promote bacteriophage production in the gut symbiont Lactobacillus reuteri[J].Cell Host&Microbe,2019,25(2):273-284.
[48] HSIAO C P,SIEBERT K J.Modeling the inhibitory effects of organic acids on bacteria[J].International Journal of Food Microbiology,1999,47(3):189-201.
[49] KAWECKI K,STANGIERSKI J,KONIECZNY P.An analysis of oxidative changes and the fatty acid profile in stored poultry sausages with liquid and microencapsulated fish oil additives[J].Molecules,2021,26(14):4293.
[50] MAURER L M,YOHANNES E,BONDURANT S S,et al.pH regulates genes for flagellar motility,catabolism,and oxidative stress in Escherichia coli K-12[J].Journal of Bacteriology,2005,187(1):304-319.
[51] BEIER R.Interactions and inhibition of pathogenic foodborne bacteria with individual dissociated organic acid species:A review[J]. Journal of Food Chemistry&Nanotechnology,2021,7:4-17.
[52] YIU J,DORWEILER B,WOO C W.Interaction between gut microbiota and toll-like receptor:From immunity to metabolism[J].Journal of Molecular Medicine,2017,95(1):13-20.
[53] SHU Q.Influence of operating conditions on reuterin production using resting cells of Limosilactobacillus reuteri DPC16[J].Fermentation,2022,8.72(3):225-231.
[54] RUI H,MUZI L,TINGTING T,et al.Construction of lactobacillus casei ghosts by holin-mediated inactivation and the potential as a safe and effective vehicle for the delivery of DNA vaccines[J].BMC Microbiology,2018,18(1-9):80.
[55] AL-SHORGANI N K N,KALIL M S,YUSOFF W M W,et al.Impact of pH and butyric acid on butanol production during batch fermentation using a new local isolate of Clostridium acetobutylicum YM1[J].Saudi Journal of Biological Sciences,2018,25(2):339-348.
[56] DAI D,QI G,WANG J,et al.Dietary organic acids ameliorate high stocking density stress-induced intestinal inflammation through the restoration of intestinal microbiota in broilers[J].Journal of Animal Science and Biotechnology,2022,13(1):124.
[57] DING X Y,BAI Y H,ZHAO Q,et al.Study on the bactericidal effect of different organic acids on Escherichia coli and its application in slaughterhouse[J]. Peertechz Publications Private Limited,2022,101(6):101842.
[58] YOUNG K M,FOEGEDING P M.Acetic,lactic and citric acids and pH inhibition of listeria monocytogenes scott a and the effect on intracellular pH[J].Journal of Applied Bacteriology,1993,74(5):515.
[59] IBRAHIM M A A,SHEHATA M N I,RADY A S M,et al.Effects of lewis basicity and acidity onσ-hole interactions in carbon-bearing complexes:A comparative ab initio study[J].International Journal of Molecular Sciences,2022,23(21):13023.