4种中药单体与丁酸钠诱导猪宿主防御肽表达的协同效果研究

doi:10.16431/j.cnki.1671-7236.2022.10.039

摘要/Abstract

摘要： 【目的】研究中药单体黄腐酚、异丹叶大黄素、去氧紫草素和毛蕊异黄酮与丁酸钠诱导猪宿主防御肽(host defense peptides,HDPs)表达的协同效果。【方法】中药单体黄腐酚、异丹叶大黄素、去氧紫草素和毛蕊异黄酮与丁酸钠单独或共同处理猪肠上皮细胞IPEC-J2和猪肺泡巨噬细胞3D4/31,通过测定HDPs的基因表达、细胞因子基因表达,以及细胞对产肠毒素性大肠杆菌(ETEC)的抑菌作用,评估中药单体与丁酸钠协同诱导HDPs表达的效果。【结果】黄腐酚与丁酸钠共同处理对HDPs的表达不表现协同作用;异丹叶大黄素与丁酸钠共同处理可协同诱导IPEC-J2细胞pEP2C和pBD3基因的表达(P＜0.01);去氧紫草素与丁酸钠共同处理可协同提高IPEC-J2细胞内pBD3和PG1-5基因的表达(P＜0.05;P＜0.01);毛蕊异黄酮与丁酸钠共同处理可协同促进IPEC-J2细胞内PG1-5、pEP2C、pBD2和pBD3基因(P＜0.05;P＜0.01)和3D4/31细胞内pBD3基因的表达(P＜0.05)。中药单体与丁酸钠共同处理对细胞因子表达无显著协作效果(P＞0.05)。除黄腐酚外,其他中药单体与丁酸钠单独或共同处理后均可提高细胞裂解产物对ETEC的抑菌能力,其中毛蕊异黄酮与丁酸钠处理对ETEC的抑制具有最佳协同效果。【结论】异丹叶大黄素、去氧紫草素和毛蕊异黄酮与丁酸钠在诱导HDPs表达、提高机体免疫和抗菌能力方面具有一定的协同作用。

关键词: 中药单体; 丁酸钠; 协同; 宿主防御肽

Abstract: 【Objective】 This study was conducted to investigate the synergistic effects of Chinese herbal monomers xanthohumol,isorhapontigenin,deoxyshikonin and calycosin and sodium butyrate on the expression of porcine host defense peptide (HDPs).【Method】 Porcine intestinal epithelial cells (IPEC-J2) and porcine alveolar macrophages (3D4/31) were treated with Chinese herbal monomers xanthohumol,isorhapontigenin,deoxyshikonin and calycosin and sodium butyrate alone or together.HDPs gene expression,cytokine gene expression and antibacterial effect of cells on enterotoxigenic Escherichia coli (ETEC) were measured to evaluate the synergistic effects of Chinese herbal monomers and sodium butyrate.【Result】 Xanthohumol and sodium butyrate had no synergistic effect on the expression of HDPs.The expression of pEP2C and pBD3 genes in IPEC-J2 cells was synergistically induced by isorhapontigenin and sodium butyrate (P＜0.01).Deoxyshikonin combined with sodium butyrate could synergically increase the expression of pBD3 and PG1-5 genes in IPEC-J2 cells (P＜0.05;P＜0.01).Treatment with calycosin and sodium butyrate could synergically promote PG1-5,pEP2C,pBD2 and pBD3 genes expression in IPEC-J2 cells (P＜0.05;P＜0.01) and pBD3 gene expression in 3D4/31 cells (P＜0.05).The co-treatment of Chinese herbal monomers and sodium butyrate had no significant cooperative effect on cytokine expression (P＞0.05).Except xanthohumol,other Chinese herbal monomers and sodium butyrate treatment alone or together could improve the antibacterial activity of cells to ETEC.Calycosin and sodium butyrate treatment showed the best synergistic effect on the inhibition of ETEC.【Conclusion】 Chinese herbal monomers isorhapontigenin,deoxyshikonin and calycosin and sodium butyrate had a certain synergistic effect on inducing HDPs expression,improving immune and antibacterial activity of the host.

Key words: Chinese herbal monomers; sodium butyrate; synergy; host defense peptide

中图分类号:

S859.3

储蓄, 张军霞, 王晶. 4种中药单体与丁酸钠诱导猪宿主防御肽表达的协同效果研究[J]. 中国畜牧兽医, 2022, 49(10): 4063-4074.

CHU Xu, ZHANG Junxia, WANG Jing. Synergistic Effect of Four Chinese Herbal Monomers and Sodium Butyrate on the Expression of Porcine Host Defense Peptide[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 4063-4074.

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