16味蒙药对金黄色葡萄球菌和大肠杆菌O8的体外抑菌效果研究

doi:10.16431/j.cnki.1671-7236.2018.12.037

摘要/Abstract

摘要：

为筛选对奶牛乳房炎主要致病菌金黄色葡萄球菌（S.aureus）和大肠杆菌O8（E.coli O₈）具有良好抑菌作用的蒙药，本试验采用牛津杯法测定金银花、黄连、何首乌、地榆、五味子、秦皮、穿心莲、甘草、蒲公英、夏枯草、虎杖、柴胡、黄柏、苦参、石榴皮、板蓝根16味蒙药的抑菌圈直径，微量稀释法和平板法测定蒙药的最小抑菌浓度（minimal inhibitory concentration，MIC）和最小杀菌浓度（minimal bactericidal concentration，MBC），棋盘法测定敏感蒙药对E.coli O8和S.aureus的协同指数（fractional inhibitory concentration index，FICI）。结果显示，黄柏、金银花、黄连、五味子、蒲公英、秦皮6味蒙药对S.aureus的抑菌圈直径为10.22~22.44 mm，MIC为15.6~250 mg/mL；五味子、金银花、黄连、秦皮4味蒙药对E.coli O8的抑菌圈直径为11.29~26.41 mm，MIC为15.6~500 mg/mL；黄柏与黄连联合用药对S.aureus的FICI为0.36，为协同作用，黄连与地榆联合用药对E.coli O8的FICI为0.48，为协同作用。综上所述，金银花、黄连、五味子、秦皮、蒲公英、黄柏6味蒙药对S.aureus综合抑菌效果达极敏或高敏；金银花、黄连、五味子、秦皮4味蒙药对E.coli O8综合抑菌效果达极敏或高敏，黄柏、黄连、秦皮在抑制S.aureus的组方中可优先考虑，黄连与地榆在抑制E.coli O8的组方中可优先考虑。

关键词: 蒙药; 金黄色葡萄球菌; 大肠杆菌O8; 最小抑菌浓度(MIC); 最小杀菌浓度(MBC); 协同指数(FICI)

Abstract:

In order to screen the Mongolian medicine which had good anti-bacterial effect on pathogenic Escherichia coli O8 (E.coli O8) and Staphylococcus aureus (S.aureus) in vitro,Oxford cup method was used to determine the inhibition zone diameter,microdilution borth method combine plate method was adopted to investigate the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Mongolian medicine,and the fractional inhibitory concent ration index (FICI) of the sensitive Mongolian medicine against E.coli O8 and S.aureus was determined by chessboard method.The results showed that the inhibition zone diameter of six Mongolian medicines to S.aureus,such as Cortex Phellodendri,Lonicera japonica,Munronia henryi Harms,Schisandra chinensis,Herba Taraxaci and Cortex Fraxini,was 10.22 to 22.44 mm and MIC was 15.6 to 250 mg/mL.The inhibition zone diameter of four Mongolian medicines to E.coli O8,such as Schisandra chinensis,Lonicera japonica,Munronia henryi Harms and Cortex Fraxini,was 11.29 to 26.41 mm and MIC was 15.6 to 500 mg/mL.The FICI of Cortex Phellodendri combined Munronia henryi Harms to S.aureus was 0.36,while that of Munronia henryi Harms combined with Sanguisorba officinalis L.to E.coli O8 was 0.48.The comprehensive bacteriostatic effects of the Cortex Phellodendri,Lonicera japonica,Munronia henryi Harms,Schisandra chinensis,Herba Taraxaci and Cortex Fraxini were extremely sensitive or highly sensitive to S.aureus.The comprehensive antibacterial effect of Schisandra chinensis,Lonicera japonica,Munronia henryi Harms and Cortex Fraxini were extremely sensitive or highly sensitive to E.coli O8.Cortex Phellodendri,Munronia henryi Harms and Cortex Fraxini were the first the choice in the group of inhibiting S.aureus,Munronia henryi Harms and Sanguisorba officinalis L. were the first the choice in the group of inhibiting E.coli O8.

Key words: Mongolian medicine; Staphylococcus aureus; Escherichia coli O8; MIC; MBC; FICI

中图分类号:

S858.23

高瑞娟, 王纯洁, 敖日格乐, 陈玉洁. 16味蒙药对金黄色葡萄球菌和大肠杆菌O8的体外抑菌效果研究[J]. 《中国畜牧兽医》, 2018, 45(12): 3614-3622.

GAO Ruijuan, WANG Chunjie, AORIGELE, CHEN Yujie. Antibacterial Effects of Sixteen Mongolian Medicines Against Pathogenic Staphylococcus aureus and Escherichia coli O8 in vitro[J]. , 2018, 45(12): 3614-3622.

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