PGD2/DP1途径对细菌感染奶牛子宫内膜组织中HMGB-1和PAFR表达的影响

doi:10.16431/j.cnki.1671-7236.2021.06.030

摘要/Abstract

摘要： 为了研究前列腺素D₂（prostaglandin D₂，PGD₂）/DP₁受体途径对大肠杆菌（Escherichia coli）和金黄色葡萄球菌（Staphylococcus aureus）感染奶牛子宫内膜组织中炎症介质HMGB-1和PAFR的表达及对组织损伤程度的影响，试验以体外培养奶牛子宫内膜组织为研究对象，应用1×10^-6 mol/L DP₁受体激动剂（BW-245C和15d-PGJ2）和等量（1×10⁶ CFU/mL）大肠杆菌、金黄色葡萄球菌共同处理奶牛子宫内膜组织，采用实时荧光定量PCR、免疫组化和HE染色法检测奶牛子宫内膜组织中HMGB-1和PAFR的表达并评价组织损伤情况。结果显示，与空白对照组相比，大肠杆菌和金黄色葡萄球菌感染奶牛子宫内膜组织中HMGB-1和PAFR表达量显著升高（P<0.05），而DP₁受体激动剂与大肠杆菌和金黄色葡萄球菌共处理奶牛子宫内膜组织中DP₁受体激动剂显著抑制奶牛子宫组织中HMGB-1和PAFR的表达（P<0.05）。HE染色结果显示，大肠杆菌与金黄色葡萄球菌感染的奶牛子宫内膜组织上皮细胞和腺上皮细胞完全脱落、坏死、崩解；而DP₁受体激动剂、大肠杆菌、金黄色葡萄球菌共同处理奶牛子宫内膜组织中，DP₁受体激动剂的加入显著减轻奶牛子宫内膜组织的损伤程度（P<0.05）。免疫组化染色法结果与以上两种方法结果一致。结果表明，PGD₂能够抑制大肠杆菌与金黄色葡萄球菌感染的奶牛子宫内膜组织中损伤相关因子HMGB-1、PAFR的表达，减轻组织损伤程度，这一作用可能是由DP₁受体所介导的。

关键词: 奶牛子宫内膜组织; 大肠杆菌; 金黄色葡萄球菌; 炎症; HMGB-1; PAFR

Abstract: The aim of this study was to investigate the effects of PGD₂/DP1 receptor pathway on the expression of inflammatory mediators HMGB-1 and PAFR,and the degree of tissue injury in the endometrium of dairy cows infected with Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus).The endometrial tissues of cows were treated with 1×10^-6 mol/L DP₁ receptor agonists (BW-245C and 15d-PGJ2) and infected with E. coli and S. aureus at a concentration of 1×10⁶ CFU/mL.The expression of HMGB-1 and PAFR was detected by Real-time quantitative PCR,immunohistochemistry,and the tissue injury was evaluated by HE staining.The results showed that the expression of HMGB-1 and PAFR of endometrium tissue in dairy cows was significantly higher than that in blank control group after E. coli and S. aureus infection at a concentration of 1×10⁶ CFU/mL (P<0.05),while the expression of HMGB-1 and PAFR in bovine endometrium was significantly inhibited by DP₁ receptor agonists (P<0.05).HE staining showed that the endometrial epithelial cells and glandular epithelial cells was completely exfoliated,necrotic and disintegrated after E.coli and S.aureus infection.While the DP₁ receptor agonists treated bovine endometrium,it significantly reduced the degree of injury of bovine endometrium (P<0.05).The results of immunohistochemical staining were consistent with those of the above two methods.The results showed that PGD₂ could inhibit the expression of HMGB-1 and PAFR and reduce the degree of tissue damage in the bovine endometrium infected with E.coli and S. aureus,which might be mediated by DP₁ receptor.

Key words: bovine endometrial tissues; E.coli; S.aureus; inflammation; HMGB-1; PAFR

中图分类号:

S857.2⁺1

杨效林, 韩润林, 毛伟, 包海霞, 刘昆, 吴金迪, 曹金山, 刘博. PGD₂/DP₁途径对细菌感染奶牛子宫内膜组织中HMGB-1和PAFR表达的影响[J]. 中国畜牧兽医, 2021, 48(6): 2169-2176.

YANG Xiaolin, HAN Runlin, MAO Wei, BAO Haixia, LIU Kun, WU Jindi, CAO Jinshan, LIU Bo. Effect of PGD₂/DP₁ Pathway on Expression of HMGB-1 and PAFR in Endometrial Tissue of Cows Infected with Bacteria[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2169-2176.

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PGD₂/DP₁途径对细菌感染奶牛子宫内膜组织中HMGB-1和PAFR表达的影响

Effect of PGD₂/DP₁ Pathway on Expression of HMGB-1 and PAFR in Endometrial Tissue of Cows Infected with Bacteria

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