不同添加水平酵母锗培养物对断奶仔猪肠道菌群、免疫力和抗氧化性能的影响

doi:10.16431/j.cnki.1671-7236.2018.07.015

摘要/Abstract

摘要：

试验旨在探究不同添加水平酵母锗培养物对断奶仔猪肠道菌群、免疫力和抗氧化性能影响。选用100头健康、体重（6.0±0.5） kg的杜×长×大三元杂交断奶仔猪，随机分成5组，每组5个重复，每个重复4头仔猪。对照组饲喂基础日粮，试验Ⅰ、Ⅱ、Ⅲ、Ⅳ组在基础日粮中分别添加酵母锗培养物15、25、35和45 mg/kg，预饲期7 d，正试期42 d，正试期分3个阶段，每阶段14 d。测定各阶段仔猪肠道乳酸杆菌及大肠杆菌数量，试验结束时测定血液免疫球蛋白含量及抗氧化性能相关指标。结果显示：与对照组相比，试验第1阶段内，试验Ⅰ、Ⅱ、Ⅲ、Ⅳ组仔猪大肠杆菌数量均呈极显著或显著下降（P<0.01；P<0.05）、乳酸杆菌数量均呈极显著或显著升高（P<0.01；P<0.05）；试验Ⅲ、Ⅳ组血清IgG含量呈极显著升高（P<0.01）；对于血清丙二醛（MDA）含量，试验Ⅱ组显著降低（P<0.05），试验Ⅲ、Ⅳ组均极显著降低（P<0.01），对于血清谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）活性及总抗氧化能力（T-AOC），试验Ⅱ组均呈显著升高（P<0.05），试验Ⅲ、Ⅳ组均呈极显著升高（P<0.01）。以上结果表明，断奶仔猪日粮中添加酵母锗培养物可有效改善肠道菌群环境、提高仔猪免疫力和抗氧化性能，结合饲养成本，建议添加量为35 mg/kg。

关键词: 酵母锗培养物; 断奶仔猪; 肠道菌群; 免疫力; 抗氧化性

Abstract:

This experiment was aimed to study the effects of different adding amounts of yeast-germanium culture on intestinal flora,immunity and antioxidant properties of weaned piglets.One hundred healthy piglets((6.0±0.5) kg) were randomly divided into 5 groups,with 5 replicates per group and 4 piglets per replicate.The control group was fed basal diet,and the content of yeast-germanium culture in basal diet of test Ⅰ,Ⅱ,Ⅲ and Ⅳ groups were 15,25,35 and 45 mg/kg,respectively.The prefeeding period was 7 days.The test period was 42 days,which was divided into 3 stages,14 days per stage.The number of Lactobacilli and Escherichia coli in intestinal tract of piglets at all stages was measured.The content of blood immunoglobulin and the related indexes of antioxidant activity were measured at the end of the test.The result shows that:Compared with the control group,in the first stage of the test,the number of Escherichia coli in all experimental groups were extremely significantly or significantly decreased (P<0.01; P<0.05),and the number of Lactobacilli in all experimental groups were extremely significantly or significantly increased (P<0.01; P<0.05).The serum IgG levels in groups Ⅲ and Ⅳ were extremely significantly increased (P<0.01).For serum MDA content,group Ⅱ was significantly decreased (P<0.05),groups Ⅲ and Ⅳ were extremely significantly decreased (P<0.01).For serum GSH-Px,SOD and T-AOC,group Ⅱ was significantly increased (P<0.05),groups Ⅲ and Ⅳ were extremely significantly increased (P<0.01).In summary,the yeast-germanium culture could effectively improve the intestinal flora environment and improve the immunity and antioxidant properties of piglets.Based on the consideration of economic cost,it was suggested that the concentration of yeast-germanium culture in weaned piglets diet was 35 mg/kg.

Key words: yeast-germanium culture; weaned piglets; intestinal flora; immunity; antioxidant properties

中图分类号:

S816.72

高凯, 李晓东, 张敏, 王震, 韩东魁, 白晓凯. 不同添加水平酵母锗培养物对断奶仔猪肠道菌群、免疫力和抗氧化性能的影响[J]. 《中国畜牧兽医》, 2018, 45(7): 1849-1855.

GAO Kai, LI Xiaodong, ZHANG Min, WANG Zhen, HAN Dongkui, BAI Xiaokai. Effects of Different Adding Amounts of Yeast-germanium Culture on Intestinal Flora, Immunity and Antioxidant Properties of Weaned Piglets[J]. , 2018, 45(7): 1849-1855.

参考文献

[1] 陈芳.抗坏血酸有机锗倍半氧化物的合成及其生物活性的研究[D].哈尔滨:黑龙江中医药大学,2008. CHEN F.Study on synthesis and biological activity of ascorbic acid sesquioxide Ge(Ⅳ) compound[D].Harbin:Heilongjiang University of Chinese Medicine,2008.(in Chinese)
[2] 郑海鹏.有机锗的生理功能及在食品中的应用[J].微量元素与健康研究,2011,28(4):65-67. ZHENG H P.The physiological function of organic germanium and application in food[J]. Studies of Trace Elements and Health,2011,28(4):65-67.(in Chinese)
[3] MATSUMOTO H,IWAFUJI H,YAMANE J,et al.Restorative effect of organic germanium compound (Ge-132) on dermal injury[J].Wound Medicine.2016,15(9):6-10.
[4] 唐兆新,史言,赵景义,等.Ge-132对蛋鸡生产性能的影响[J].黑龙江畜牧兽医,1995,2:1-4. TANG Z X,SHI Y,ZHAO J Y,et al.Effect of Ge-132 on performance of laying hens[J].Heilongjiang Animal Science and Veterinary Medicine,1995,2:1-4.(in Chinese)
[5] 袁缨,温萍,郭东新,等.有机和无机锗对艾维因肉仔鸡脂类代谢的影响[J].沈阳农业大学学报,2000,31(2):203-206. YUAN Y,WEN P,GUO D X,et al.Effects of organic and inorganic germanium on lipid metabolism of broilers[J].Journal of Shenyang Agricultural University,2000,31(2):203-206.(in Chinese)
[6] 刘素洁,关乃鹏.两种锗化合物对肉仔鸡生产性能的影响[J].辽宁畜牧兽医,2002,2:3-5. LIU S J,GUAN N P.Effects of two germanium compounds on production performance of broilers[J].Liaoning Journal of Animal Husbandry and Veterinary Medicine,2002,2:3-5.(in Chinese)
[7] 田伟,陈晓东.羟基肉桂酸锗氮桥环化合物在乌鸡饲料中的应用[J].安徽农业科学,2012,40(8):4592-4594. TIAN W,CHEN X D.The application of germatranes of hydroxyl substituted cinnamic acid compounds in the diet of gallus domesticus brisson[J].Journal ofAnhui Agricultural Sciences,2012,40(8):4592-4594.(in Chinese)
[8] 兰喜.锗~(-132)对奶牛泌乳量及乳汁品质影响的研究[D].兰州:甘肃农业大学,2001. LAN X.Effects of germanium^-132 on milk production and composition in Holstein dairy cows[D].Lanzhou:Gansu Agricultural University,2001.(in Chinese)
[9] 张连忠.酵母培养物对雏鸡生长性能、免疫器官发育和血清相关激素的影响[J].中国畜牧兽医,2011,38(4):33-37. ZHANG L Z.Effects of yeast culture on the growth performance,immune organs development and serum related hormones concentration of laying chicks[J].China Animal Husbandry & Veterinary Medicine,2011,38(4):33-37.(in Chinese)
[10] 何若钢,肖正中,周晓情,等.日粮中添加酵母培养物对断奶仔猪生长性能的影响[J].中国畜牧兽医,2009,36(3):25-27. HE R G,XIAO Z Z,ZHOU X Q,et al.The influence of yeast cultures in the dietary on performance of weaning piglets[J].China Animal Husbandry &Veterinary Medicine,2009,36(3):25-27.(in Chinese)
[11] 王典,王加启,宋丽华.日粮中添加酵母或酵母培养物对奶牛生产性能的影响[J].中国畜牧兽医,2012,39(5):82-85. WANG D,WANG J Q,SONG L H.Effect of a supplemental yeast or yeast culture on milk production of dairy cow[J].China Animal Husbandry & Veterinary Medicine,2012,39(5):82-85.(in Chinese)
[12] 郭小华,刘明,李文辉,等.日粮中添加酵母培养物对肉仔鸡生长性能、肉品质和肠道菌群的影响[J].中国畜牧兽医,2017,44(6):1673-1679. GUO X H, LIU M,LI W H,et al.Effects of yeast culture on growth performance,meat quality and intestinal microflora of broilers[J].China Animal Husbandry & Veterinary Medicine, 2017,44(6):1673-1679.(in Chinese)
[13] 丘权,詹志春,周樱,等.饲料中微生态制剂应用研究[J].饲料工业,2016,37(2):55-58. QIU Q,ZHAN Z C,ZHOU Y,et al.Research of probiotics application in feed[J].Feed Industry,2016,37(2):55-58.(in Chinese)
[14] 白晓凯,李艳娇,金英海,等.酵母菌发酵有机米糠富锗的条件优化[J].饲料工业,2016,37(2):47-50. BAI X K,LI Y J,JIN Y H,et al.Optimization of germanium accumulation conditions by yeast to fermentthe organic rice bran[J].Feed Industry, 2016,37(2):47-50.(in Chinese)
[15] 谯仕彦,侯成立,曾祥芳.乳酸菌对猪肠道屏障功能的调节作用及其机制[J].动物营养学报,2014,26(10):3052-3063. JIAO S Y,HOU C L,ZENG X F.Regulation and mechanism of lactic acid bacteria on porcine intestinal barrier function[J].Chinese Journal of Animal Nutrition,2014,26(10):3052-3063.(in Chinese)
[16] LEE S H,SHINDE P,CHOI J,et al.Effects of dietary iron levels on growth performance,hematological status,liver mineral concentration,fecal microflora,and diarrhea incidence in weaning pigs[J].Biological Trace Element Research,2008,126(1):57-68.
[17] JONES S E,VERSALOVIC J.Probiotic Lactobacillus reuteri biofilms produce antimicrobial and anti-inflammatory factors[J].BMC Microbiol,2009,9(2):1-9.
[18] 侯冠彧,周汉林,荀文娟,等.几种常用饲料添加剂对断奶仔猪肠道菌群调控的研究进展[J].中国畜牧兽医,2016,43(11):2939-2944. HOU G Y,ZHOU H L,XUN W J,et al.Research advances on intestinal microflora regulationin by several feed additives in piglets[J].China Animal Husbandry & Veterinary Medicine,2016,43(11):2939-2944.(in Chinese)
[19] GRELA E R,SOBOLEWSKA S,KOWALCZUKVASILEV E,et al.Effect of dietary inulin source on piglet performance,immunoglobulin concentration,and plasma lipid profile[J].Bulletin of the Veterinary Institute in Pulawy,2014,58(3):453-458.
[20] 张娜,孙高超.免疫球蛋白功能和应用研究[A].中国畜牧兽医学会2009学术年会论文集(下册)[C].中国畜牧兽医学会,2009. ZHANG N,SUN G C.Function and Application of Immunoglobulin[A].China Animal Husbandry and Veterinary Institute of 2009 Academic Essays (Part Ⅱ)[C].Chinese Society of Animal Husbandry and Veterinary Medicine,2009.(in Chinese)
[21] 陈越,金久善,董梅,等.羧乙基锗倍半氧化物对雏鸡免疫功能的影响[J].北京农业大学学报,1995,S1:34-42. CHEN Y,JIN J S,DONG M,et al.Effect of Ge-132 on immunization in healthy and MDV-infected broil-ers[J].Journal of China Agricultural University,1995,S1:34-42.(in Chinese)
[22] JUNG M,PARK H,PARK J,et al.Effects of germanium biotite supplement on immune responses of vaccinated mini-pigs to foot-and-mouth disease virus challenge[J].Immunological Investigations,2015,44(1):101-112.
[23] 刘国艳,柴春彦,吕世文,等.锗-132对高氟低硒地区奶牛自由基代谢的影响[J].中国兽医杂志,2000,26(3):10-13. LIU G Y,CHAI C Y,LV S W,et al.Effects of Ge-132 on antioxidant function of dairy cattle[J].Chinese Journal of Veterinary Medicine,2000,26(3):10-13.(in Chinese)
[24] 王明臣,王俊萍,李玉山,等.有机锗干预大鼠矽肺形成及其抗氧化应激机制的研究[J].中国职业医学,2003,30(1):43-45. WANG M C,WANG J P,LI Y S,et al.Studies on interference of organic germanium in the formation of silicosis of rats and its mechanism of antioxidative stress[J].China Occupational Medicine,2003,30(1):43-45.(in Chinese)
[25] 杨利,卢立志,黄仁录,等.Ge-132对蛋鸭机体抗氧化能力的影响[J].中国兽医学报,2007,27(5):770-773. YANG L,LU L Z,HUANG R L,et al.Effects of Ge-132 on antioxidant function of laying ducks[J].Chinese Journal of Veterinary Science,2007,27(5):770-773.(in Chinese)
[26] 丁雯.锗对实验性类风湿性关节炎大鼠抗氧化功能的影响[D].上海:第二军医大学,2008. DING W.Germanium on experimental rheumatoid arthritis in rats antioxidant function[D].Shanghai:Second Military Medical University,2008.(in Chinese)