厚朴总酚抗氧化作用及其机制研究进展

doi:10.16431/j.cnki.1671-7236.2016.09.026

摘要/Abstract

摘要：

厚朴总酚是一种提取于传统中药厚朴的酚类混合物，主要包含厚朴酚、和厚朴酚两种有效活性成分，具有抗病毒、抗菌、抗炎和抗腹泻等多种生物学功能，而厚朴总酚的抗氧化作用则是其发挥生物学功能的重要基础。作者对厚朴总酚通过清除自由基、增强抗氧化酶活性和阻断脂质过氧化3种途径发挥其抗氧化作用的机制进行了综述，旨在为深入研究其抗氧化作用及作为“绿色”抗氧化剂应用于畜牧生产提供参考。

关键词: 厚朴总酚; 抗氧化; 机制

Abstract:

Total phenols from Magnolia officinalis,one kind of phenolic mixtures,is extracted from Magnolia officinalis and mainly contains Magnlo and Honokial,which has a wide range of biological functions,such as anti-viral,anti-microbial,anti-inflammatory and anti-diarrhea activities,ect.The antioxidant activity is thought as a key foundation of these above-mentioned function roles.In this paper,antioxidant function roles of total phenols from Magnolia officinalis are reviewed through three mechanisms which included eliminating superoxide anion free radical,increasing the activity of antioxidation enzyme and inhibiting lipid peroxidation.This review will provide a reference for further researching about its antioxidant function roles and being used in livestock production as a green antioxidant.

Key words: total phenols from Magnolia officinalis; antioxidant; mechanisms

中图分类号:

S816.7

曹蓉, 谢堃, 侯德兴, 贺喜. 厚朴总酚抗氧化作用及其机制研究进展[J]. 《中国畜牧兽医》, 2016, 43(9): 2395-2400.

CAO Rong, XIE Kun, HOU De-xing, HE Xi. Research Advance on Antioxidant Function Roles and Its Mechanism of Total Phenols from Magnolia officinalis[J]. , 2016, 43(9): 2395-2400.

参考文献

[1] 瞿庆喜,朱庆亚,喻凯,等.凹叶厚朴中具有α-葡萄糖苷酶抑制活性的成分[J].应用与环境生物学报,2009,15(6):78-79.
[2] 程迪,王佳丽,周子涛,等.厚朴总酚对肉仔鸡生长性能、免疫功能及抗氧化能力的影响[J].饲料研究,2015,15:1-4.
[3] 李晟,陈晓阳,邹志,等.加味厚朴温中汤对泄泻湿阻证大鼠胃肠道P物质和白细胞[J].中国中西医结合消化杂志,2009,17(5):296-299.
[4] 闵玲,朱小牧,胡丽娜.和厚朴酚在肿瘤治疗中的研究进展[J].华西药学杂志,2010,25(1):113-115.
[5] 国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2010.
[6] 张春霞,杨立新,余星,等.种源、产地及采收树龄对厚朴药材质量的影响[J].中国中药杂志,2009,34(19):2431-2437.
[7] 国家药典委员会.中国药典[M].北京:中国医药科技出版社,2015.
[8] 张勇,唐方.厚朴酚药理作用的最新研究进展[J].中国中药杂志,2012,37(23):3526-3530.
[9] 李锐,陈中兰,肖宏,等.厚朴中厚朴酚及和厚朴酚提取工艺的优化研究[J].中国药业,2011,20(10):47-48.
[10] 冯华,申桂霞,邹孔强.黔北产厚朴中和厚朴酚的提取工艺研究[J].医药导报,2010,7:918-919.
[11] 叶锦霞,林珊,曾建伟,等.闽产厚朴叶中厚朴酚、和厚朴酚提取工艺研究[J].福建中医学院学报,2010,4:23-26.
[12] 姜宁,刘晓鹏,田心鹏,等.微波辅助提取厚朴叶中厚朴酚及和厚朴酚的研究[J].林业实用技术,2007,9:8-10.
[13] 杨素荣,莫启武,杨承鸿,等.1000 L×2超临界CO₂装置萃取厚朴有效成分的工业化研究[J].中药材,2002,8:584-585.
[14] 张忠义,王桂芳,雷正杰.超临界CO₂萃取厚朴的主要成分[J].中国医院药学杂志,2001,7:17-18.
[15] 孙爱玲,冯蕾,柳仁民.高速逆流色谱分离制备厚朴的有效成分厚朴酚与和厚朴酚[J].分析化学,2005,7:1016-1018.
[16] 李国章.和厚朴酚的药理作用[J].亚太传统医药,2012,8(1):44-45.
[17] 张淑洁,钟凌云.厚朴化学成分及其现代药理研究进展[J].中药材,2013,36(5):838-843.
[18] 林琳,丁一.和厚朴酚最新药理学研究进展与评述[J].中药药理与临床,2011,1:110-112.
[19] 王丹,冯芳.厚朴酚与和厚朴酚的体内药物分析方法及药代动力学研究近况[J].药学进展,2011,35(9):396-400.
[20] 马莎莎,邵玉凤,吴祥猛,等.LC-MS/MS法研究厚朴酚与和厚朴酚在大鼠体内的药动学行为[J].质谱学报,2013,34(1):23-28.
[21] Ogata M,Hoshi M,Shimotohno K,et al.Antioxidant activity of magnolol,honokiol,and related phenolic compounds[J].J Am Oil Chem Soc,1997,74(5):562.
[22] Bao Z J,Yang X Q,Ding Z T,et al.Simultaneous determination of magnolol and honokiol by UV spectrophotometry and study on free radical scavenger activity[J].Nat Prod Res Devel,2004,16(5):435-438.
[23] Schuhly W,Hufner A,Pferschy-Wenzig E M,et al.Design and synthesis of ten biphenyl-neolignan derivatives and their in vitro inhibitory potency against cyclooxygenase-1/2 activity and 5-lipoxygenase-mediated LTB4-formation[J].Bioorg Med Chem,2009,17(13):4459-4465.
[24] Dikalov S,Losik T,Arbise J L.Honokiol is a potent scavenger of superoxide and peroxyl radicals[J].Biochem Pharmacol,2008,76(5):589-596.
[25] Burton G J,Jauniaux E.Oxidative stress[J].Best Practice & Research Clinical Obstetrics & Gynaecology,2011,25(3):287-299.
[26] Bedard K,Krause K H,The NOX family of ROS-generating NADPH oxidases:Physiology and pathophysi-ology[J].Physiological Reviews,2007,87(1):245-313.
[27] Mikhailov V F,Mazurik V K,Burlakova E B.Signal function of the reactive oxygen species inregulatory networks of the cell reaction to damaging effects:Contribution of radiosensitivity and genome instability[J].Radiatsionnaia Biologiia,Radioecologiia/Rossiiskaia Akademiia Nauk,2003,43(1):5.
[28] Simon H U,Haj-Yehia A,Levi-Schaffer F.Role of reactive oxygen species (ROS) in apoptosis induction[J].Apoptosis,2000,5(5):415-418.
[29] 孟洁,胡迎芬,胡博路,等.厚朴抗氧化作用研究[J].中国油脂,2000,25(4):30-32.
[30] 郝庆红,陈冠华,冯雅琪,等.厚朴中清除氧自山基主要有效成分的确定[J].河北大学学报:自然科学版,2005,25(2):164-178.
[31] 保志娟,杨雪琼,邹永明,等.厚朴酚与和厚朴酚清除DPPH·的作用[J].云南大学学报,2005,27(1):60-63.
[32] Sun J K,Do Y K,Yeong S K,et al.Peroxyl radical scavenging capacityofextracts and isolated components from selected medicinal plants[J].Arch Pharm Res,2010,33(6):867.
[33] Zhao C,Liu Z Q.Comparison of antioxidant abilities of magnolol and honokiol to scavengeradicals and to protect DNA[J].Bio-chemie,2011,93:1755.
[34] 王沂,吴翔,翁新楚,厚朴酚对果蝇寿命和繁殖力的影响[J].四川动物,2006,25(4):700-702.
[35] 黄信全,王从平,陈开广,等.厚朴酚对大鼠局灶性脑缺血再灌注损伤保护作用的研究[J].中国神经免疫学和神经病学杂志,2007,14(2):118-119.
[36] 张广钦,陈世忠,郝雪梅,等.厚朴酚对脑缺血的保护作用[J].中国药理学通报,2003,9:1020-1023.
[37] 郝庆红,陈冠华,冯雅琪,等.和厚朴酚对D-半乳糖衰老模型小鼠抗氧化作用的研究[J].中国中药杂志,2009,34(6):798-800.
[38] Saito J,Shibuya K,Nagasc H.Anti-clastogenic effect of magnolol on benzo(a)pyrene-induced clastogenicity in mice[J].Food Chem Toxicol,2008,46(2):694.
[39] 庞月兰.厚朴酚与和厚朴酚抗氧化和抗腹泻功能研究[D].长沙:湖南农业大学,2010.
[40] 林谦,邱磊,云龙,等.核因子E2相关因子2调控机体抗氧化途径特性及其与畜禽的健康和肉品质的关系[J].动物营养学报,2014,6:1421-1429.
[41] Schreibelt G,Horssen J,Rossum S,et al.Therapeutic potential and biological role of endogenous antioxidant enzymes inmultiple sclerosis pathology[J].Brain Res Rev,2007,56(2):322-330.
[42] Jain A K,Jaiswal A K.GSK-3 beta acts upstream of Fyn kinase inregulation of nuclear export and dergradation of NF-E2 related factor 2[J].J Biol Chem,2007,282(22):16502-16510.
[43] 李煌元,石年.Keap-Nrf2/ARE通路在分予毒理学中的研究进展[J].国外医学卫生学分册,2006,33(3):129-135.
[44] 张利娜,王红巧,张黎明,等.氧化应激在硒缺乏致鸡胰岛β细胞功能损伤作用[J].中国家禽,2009,31(19):16-19.
[45] Park E J,Zhao Y Z,Bae K,et al.Protective effects of honokiol and magnolol on tertiary butyl hydroperoxide-or D-galactsamine-induced toxicity in rat primary hepatocytes[J].Planta Medica,2003,69(1):33-37.
[46] Li H B,Gao J M,Ying X X,et al.Protective effect ofmagnolol on TBHP-induced injury in H460 cells partially via a p53 depend-ent mechanism[J]. Arch Pharm Res,2007,30(7):850.
[47] 冯雅琪,吴国江,郝庆红,等.厚朴酚抗脂质过氧化作用研究[J].河北大学学报,2005,25(1):52-54.
[48] 向华.厚朴酚抗金黄色葡萄球菌作用机制的研究[D].长春:吉林大学,2010.
[49] 金晶.中药和厚朴酚抗真菌活性及其作用机制的初步研究[D].长春:吉林大学,2011.
[50] 李园莉.厚朴酚与和厚朴酚高效提制技术及抗菌活性研究[D].长沙:湖南农业大学,2009.
[51] 邓燕莉.厚朴酚与和厚朴酚抗腹泻作用及分子机理研究[D].长沙:湖南农业大学,2012.