关键词: 局灶性脑缺血后适应; 动物模型; 建立; 评估

Abstract: The success rate, stability and protection of the three animal models for focal cerebral ischemia postconditioning (IPOC) in rats with neuroprotective effects were compared, which established good animal models for the study of IPOC mechanism. SD rats were randomly divided into three groups, including model group 1 (middle cerebral artery coagulation craniotomy+bilateral carotid artery occlusion 30 min), model group 2 (middle cerebral artery occlusion (MCAO) 60 min), model group 3 (MCAO 100 min), and then three groups were divided into three subgroups, named Sham subgroup, ischemia subgroup and ischemic postconditioning subgroup, each subgroup had 10 rats. Neurological defect scores were done in 2 and 24 h post perfusion, and brains were stained by 2,3,5-trichloro tetrazolium (TTC) to determine the percentage of infarct volume, and statistical analysis in 24 h post perfusion. The results showed that these models all experienced varying degrees of neurologicalsigns, and IPOC reduced the infarct volume and improved neurological function at different degrees. Among these, modeling with electrocoagulation ischemia 30 min was the best. Electrocoagulation adapted model of ischemia 30 min decreased infarct volume by 42.9%, and MCAO model of ischemic postconditioning 60 min decreased the infarct volume by 15.9%, while suture method post-ischemic adapted model 100 min decreased infarct volume by 33.4%. Moreover, the coefficient of variation of infarct volume for electrocoagulation adapted model of ischemia 30 min was the lowest. Craniotomy electrocoagulation ischemia reperfusion 30 min ischemia 30 s/ischemia postconditioning 10 s,then repeated three times, not only the protects of IPOC model was stronger, but also modeling had higher success rate, good stability and could be used as better animal models for research of IPOC mechanism.

Key words: focal cerebral ischemia postconditioning; animal model; establishment; assessmen