口蹄疫、猪瘟、高致病性猪繁殖与呼吸综合征3种疫苗同步两点免疫的田间应用试验

doi:10.16431/j.cnki.1671-7236.2017.08.033

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (8): 2450-2457.doi: 10.16431/j.cnki.1671-7236.2017.08.033

口蹄疫、猪瘟、高致病性猪繁殖与呼吸综合征3种疫苗同步两点免疫的田间应用试验

覃勇¹, 赵聪¹, 黄文炳², 李佳荣³, 唐云姣¹, 班雪花⁴, 施开创⁵

1. 百色市动物疫病预防控制中心, 百色 533000;
2. 平果县动物疫病预防控制中心, 平果 531400;
3. 百色农业学校, 百色 533000;
4. 田东县动物疫病预防控制中心, 田东 531500;
5. 广西动物疫病预防控制中心, 南宁 530001

收稿日期:2017-01-18 出版日期:2017-08-20 发布日期:2017-08-18
通讯作者: 施开创 E-mail:shikaichuang@126.com
作者简介:覃勇(1974-),男,广西百色人,学士,高级兽医师,研究方向:动物疫病预防控制,E-mail:bsqin2003@163.com
基金资助:
广西科学研究与技术开发计划项目（桂科转14125004-22）；广西水产畜牧科技项目（桂渔牧科201528017）

Application Experiment in the Field of FMD,CSF and HP-PRRS Vaccines Simultaneously Vaccinated at Two Different Sites

QIN Yong¹, ZHAO Cong¹, HUANG Wen-bing², LI Jia-rong³, TANG Yun-jiao¹, BAN Xue-hua⁴, SHI Kai-chuang⁵

1. Baise Center for Animal Disease Control and Prevention, Baise 533000, China;
2. Pingguo Center for Animal Disease Control and Prevention, Pingguo 531400, China;
3. Baise Agricultural School, Baise 533000, China;
4. Tiandong Center for Animal Disease Control and Prevention, Tiandong 531500, China;
5. Guangxi Center for Animal Disease Control and Prevention, Nanning 530001, China

Received:2017-01-18 Online:2017-08-20 Published:2017-08-18

摘要/Abstract

摘要：

为探索农村散养猪口蹄疫（foot-and-mouth disease，FMD）、猪瘟（classical swine fever，CSF）、高致病性猪繁殖与呼吸综合征（highly pathogenic porcine reproductive and respiratory syndrome，HP-PRRS））疫苗的最佳免疫程序，在2个镇开展田间免疫试验，A镇采用"332法"免疫程序，即先用FMD和CSF疫苗同时在颈部分两侧注射，间隔14 d后再用HP-PRRS疫苗注射；B镇采用"321法"免疫程序，即将CSF和HP-PRRS疫苗混合后作一针在颈部一侧注射，FMD疫苗作一针在颈部另一侧注射。应用ELISA方法检测FMDV、CSFV和HP-PRRSV抗体，应用RT-PCR方法检测FMDV、CSFV和HP-PRRSV病原；统计、分析生猪免疫密度、免疫副反应率、生猪死淘率、散养猪抗体阳性率、屠宰猪抗体阳性率、死淘猪病原阳性率、屠宰猪病原阳性率等指标。结果显示，应用"321法"的B镇与应用"332法"的A镇相比，其HP-PRRS免疫密度、散养猪CSFV和HP-PRRSV抗体阳性率、屠宰猪HP-PRRSV抗体阳性率均显著增加（P<0.05），散养猪死淘率、HP-PRRSV阳性率均显著下降（P<0.05），而其他主要考核指标则差异不显著（P>0.05）。以上结果表明，应用"321法"免疫程序的免疫效果优于"332法"，值得在临床推广应用。

关键词: 口蹄疫; 猪瘟; 高致病性猪繁殖与呼吸综合征; 免疫程序; 田间试验; 免疫效果; 评估

Abstract:

In order to explore the optimal immune procedure of foot-and-mouth disease (FMD), classical swine fever (CSF) and highly pathogenic porcine reproductive and respiratory syndrome (HP-PRRS) vaccines in backyard pigs in rural areas, field immune experiment was carried out in two towns. Town A used "332" immune procedure, namely, FMD vaccine and CSF vaccine were simultaneously injected at different sides of the neck, and HP-PRRS vaccine was injected 14 days later. Town B used "321" immune procedure, namely, HP-PRRS vaccine and CSF vaccine were mixed together and injected in one side of the neck, and FMD vaccine was injected in the other side of the neck. FMD, CSF and HP-PRRS antibodies were detected by ELISA, and FMDV, CSFV and HP-PRRSV were detected by RT-PCR. The assessment indexes, including the immune density, immune side-reaction rate, death rate of pigs, positive rate of antibody of backyard pigs, positive rate of antibody of slaughter pigs, positive rate of pathogen of backyard pigs, positive rate of pathogen of slaughter pigs and so on, were collected and analyzed. The results showed that, compared with town A used "332" immune procedure, the HP-PRRS immune density, the positive rate of CSF and HP-PRRS antibody of backyard pigs, the positive rate of HP-PRRS antibody of slaughter pigs were significantly increased (P<0.05), while the death rate and the positive rate of HP-PRRSV of slaughter pigs were significantly decreased (P<0.05) in town B used "321" immune procedure. The other major assessment indexes had no significant difference (P>0.05). The results indicated that the immune efficacy of "321" immune procedure was better than that of "332", and it was worthy to be applied in clinical practice.

Key words: foot-and-mouth disease (FMD); classical swine fever (CSF); highly pathogenic porcine reproductive and respiratory syndrome (HP-PRRS); immune procedure; field experiment; immune efficacy; evaluation

中图分类号:

S858.28

覃勇, 赵聪, 黄文炳, 李佳荣, 唐云姣, 班雪花, 施开创. 口蹄疫、猪瘟、高致病性猪繁殖与呼吸综合征3种疫苗同步两点免疫的田间应用试验[J]. 《中国畜牧兽医》, 2017, 44(8): 2450-2457.

QIN Yong, ZHAO Cong, HUANG Wen-bing, LI Jia-rong, TANG Yun-jiao, BAN Xue-hua, SHI Kai-chuang. Application Experiment in the Field of FMD,CSF and HP-PRRS Vaccines Simultaneously Vaccinated at Two Different Sites[J]. , 2017, 44(8): 2450-2457.

参考文献

[1] 中华人民共和国农业部及财政部.2016年国家动物疫病强制免疫计划[J].四川畜牧兽医,2016,5:14-16.
[2] Loving C L, Osorio F A, Murtaugh M P, et al. Innate and adaptive immunity against porcine reproductive and respiratory syndrome virus[J]. Vet Immunol Immunopathol, 2015, 167(1-2):1-14.
[3] Gu W, Guo L, Yu H, et al. Involment of CD16 in antibody-dependent enhancement of porcine reproductive and respiratory syndrome virus infection[J]. J Gen Virol, 2015, 96(Pt 7):1712-1722.
[4] 王振华,曹晓涵,宋禾,等.猪繁殖与呼吸综合征病毒的危害及免疫策略[J].中国畜牧兽医,2014, 41(2):239-244.
[5] Wang X, Mu G, Dang R, et al. Up-regulation of IL-10 upon PRRSV vaccination impacts on the immune response against CSFV[J]. Vet Microbiol, 2016, 197:68-71.
[6] Suradhat S, Kesdangsakonwut S, Sada W, et al. Negative impact of porcine reproductive and respiratory syndrome virus infection on the efficacy of classical swine fever vaccine[J]. Vaccine, 2006, 24(14):2634-2642.
[7] von Rosen T, Lohse L, Nielsen J, et al. Classical swine fever virus infection modulates serum levels of INF-α, IL-8 and TNF-α in 6-month-old pigs[J]. Res Vet Sci, 2013, 95(3):1262-1267.
[8] Muñoz-González S, Ruggli N, Rosell R, et al. Postnatal persistent infection with classical swine fever virus and its immunological implications[J]. PLoS One, 2015, 10(5):e0125692.
[9] Tarradas J, de la Torre M E, Rosell R, et al. The impact of CSFV on the immune response to control infection[J]. Virus Res, 2014, 185:82-91.
[10] De Clercq K, Koenen F, Strobbe R, et al. Simultaneous vaccination of piglets against foot-and-mouth disease and classical swine fever[J]. Vet Microbiol, 1989, 20(3):215-221.
[11] 胡杰,张步娴,屈素洁,等.不同免疫方式对仔猪免疫高致病性猪繁殖与呼吸综合征、猪瘟、猪口蹄疫三种疫苗抗体水平的影响[J]. 中国动物传染病学报,2014, 22(5):61-67.
[12] 张长龙,严宝英,贾文孝,等.猪瘟、猪口蹄疫、高致病性猪蓝耳病疫苗有效免疫试验初探[J]. 动物医学进展,2009,30(7):101-103.
[13] 卢景,陆承平,段振华.口蹄疫、猪瘟和高致病性猪蓝耳病3种疫苗不同组合对猪的免疫效果评价[J]. 畜牧与兽医,2012, 44(1):76-79.
[14] 窦鹏勋,葛建强,张勇,等.3种疫苗单独和联合免疫对猪抗体水平的影响[J]. 安徽农业科学,2009, 37(31):15259-15261.
[15] 马睿麟,蔡金山,沈艳丽,等.猪瘟、口蹄疫、猪繁殖与呼吸综合征等多种疫苗免疫试验[J].畜牧与兽医,2010, 42(10):79-82.
[16] 陈文武,马长宾,崔蕾,等.FMD、HP-PRRS及CSF三种疫苗不同免疫组合同时分点免疫的抗体水平检测[J]. 黑龙江畜牧兽医,2016,3:95-98.
[17] 任宇斓,于厚军,蒋文生,等.猪口蹄疫、猪瘟、高致病性猪蓝耳病三种疫苗不同组合同时分点免疫效果观察与分析[J].中国动物检疫,2011, 28(5):67-71.
[18] 陶得和,漆晶晶,白文丽,等.高致病性蓝耳病疫苗、猪瘟、口蹄疫疫苗同时免疫试验报告[J]. 河南畜牧兽医,2010, 31(6):6-8.
[19] 周建国,张应国,段博芳,等.口蹄疫、猪瘟和高致病性猪蓝耳病三种疫苗同步两点免疫新技术研究与应用[J]. 中国畜牧兽医文摘,2016, 32(7):100-101.
[20] 赵云堂,夏桂林,谭建春.猪瘟、高致病性猪蓝耳病疫苗组合与猪口蹄疫O型灭活苗同时分点免疫效果评价[J]. 中兽医医药杂志,2013,6:43-44.

口蹄疫、猪瘟、高致病性猪繁殖与呼吸综合征3种疫苗同步两点免疫的田间应用试验

Application Experiment in the Field of FMD,CSF and HP-PRRS Vaccines Simultaneously Vaccinated at Two Different Sites

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	张芳源, 蔺雅婷, 杨大为, 陈虎, 李桂梅, 单虎. 非洲猪瘟病毒P30蛋白原核表达及间接ELISA检测方法建立[J]. 中国畜牧兽医, 2023, 50(5): 2012-2022.
[2]	侯浩宇, 郑南南, 吴宏举, 陈寅龙, 李潮, 张昂克, 姬鹏超, 万博, 杜永坤, 张改平. 非洲猪瘟病毒p30蛋白单克隆抗体制备和鉴定[J]. 中国畜牧兽医, 2023, 50(3): 1241-1249.
[3]	李朋霏, 宋晓明, 周保琨, 曹志, 张洪亮, 马清霞, 单虎. 用于非洲猪瘟病毒核酸检测的假病毒阳性对照品的制备和标定[J]. 中国畜牧兽医, 2023, 50(2): 647-655.
[4]	张凯, 张春平, 戈胜强, 孙春喜, 程杰, 伏春雨, 王刚, 牛星, 彭军. 非洲猪瘟病毒p22蛋白表达、多克隆抗体制备及间接ELISA方法的建立[J]. 中国畜牧兽医, 2023, 50(1): 270-279.
[5]	张梦杨, 何健, 刘阳坤, 姚伦广. 非洲猪瘟病毒p37蛋白生物信息学分析及其多克隆抗体制备[J]. 中国畜牧兽医, 2023, 50(1): 300-309.
[6]	杨涛, 张敏婕, 徐然, 张涵, 张萌萌, 吴克亮. 应用BLUP方法对猪繁殖性状和生长性状遗传评估的研究[J]. 中国畜牧兽医, 2022, 49(8): 2971-2981.
[7]	徐娅玲, 张霁辉, 牛熙, 李升, 黄世会, 冉雪琴, 王嘉福. 非洲猪瘟病毒P72蛋白合成肽疫苗的构建及其免疫效力评估[J]. 中国畜牧兽医, 2022, 49(8): 3083-3090.
[8]	陈世钰, 崔帅, 蒋亚君, 鑫婷, 王洋, 郝玉欣, 黄建新, 郭晓宇, 朱鸿飞, 吴佳俊, 贾红. 非洲猪瘟病毒感染后猪外周血淋巴细胞Toll样受体信号通路的lncRNA-mRNA分析[J]. 中国畜牧兽医, 2022, 49(7): 2677-2687.
[9]	张文燕, 王亚文, 袁晨, 冯亚文, 滕召剑, 商佳亮, 逯纪成, 宋勤叶. 基于非洲猪瘟病毒截短p72蛋白的间接ELISA抗体检测方法的建立[J]. 中国畜牧兽医, 2022, 49(7): 2688-2697.
[10]	程晶, 刘文晓, 王晓玥, 王丽萍, 江波, 李永清. 非洲猪瘟诊断方法研究进展[J]. 中国畜牧兽医, 2022, 49(5): 1985-1993.
[11]	罗颖, 张先东, 李慧, 李樵锋, 刘伟琪, 付静静, 黄璐琦, 邓桦, 杨鸿. 急性非洲猪瘟脑损伤及NF-κB介导脑水肿形成机制研究[J]. 中国畜牧兽医, 2022, 49(3): 1048-1056.
[12]	张艳敏, 周亚南, 曹磊, 田园, 刘旭平, 谭文松, 赵亮. 猪瘟病毒E2-GM-CSF融合蛋白在HEK293T细胞中的表达和免疫原性分析[J]. 中国畜牧兽医, 2022, 49(2): 669-676.
[13]	许春梅, 邵明珠, 王心悦, 林佳迪, 郭建雄, 张祥胤, 童德文, 梁瑞英, 赵晓民. 非洲猪瘟病毒D250R蛋白生物信息学分析及多克隆抗体制备[J]. 中国畜牧兽医, 2022, 49(12): 4745-4755.
[14]	刘欢, 张蒙蒙, 崔一芳, 刘静, 张靖菊, 陈登金, 李秀波, 李存, 徐飞. 复方季铵盐泡沫消毒剂成泡性能的优化及对病毒的杀灭效果评价[J]. 中国畜牧兽医, 2022, 49(12): 4866-4877.
[15]	崔帅, 王洋, 郭晓宇, 陈世钰, 蒋亚君, 庞忠宝, 黄建新, 孟凡峰, 姜一曈, 朱鸿飞, 贾红. 利用酵母双杂交技术筛选和鉴定非洲猪瘟病毒E120R蛋白的互作宿主蛋白[J]. 中国畜牧兽医, 2022, 49(11): 4139-4149.