复方季铵盐泡沫消毒剂成泡性能的优化及对病毒的杀灭效果评价

doi:10.16431/j.cnki.1671-7236.2022.12.034

中国畜牧兽医 ›› 2022, Vol. 49 ›› Issue (12): 4866-4877.doi: 10.16431/j.cnki.1671-7236.2022.12.034

复方季铵盐泡沫消毒剂成泡性能的优化及对病毒的杀灭效果评价

刘欢^1,2, 张蒙蒙², 崔一芳³, 刘静², 张靖菊², 陈登金⁴, 李秀波², 李存¹, 徐飞²

1. 天津农学院动物科学与动物医学学院, 天津 300384;
2. 中国农业科学院饲料研究所, 国家饲料药物基准实验室, 北京 100081;
3. 北京市农林科学院畜牧兽医研究所, 北京 100089;
4. 中国农业大学动物医学院, 北京 100193

收稿日期:2022-05-26 出版日期:2022-12-05 发布日期:2022-12-01
通讯作者: 李存, 徐飞 E-mail:hhlicun@163.com;xufei@caas.cn
作者简介:刘欢,E-mail:liuhuanjia@foxmail.com;张蒙蒙,E-mail:1612422443@qq.com。
基金资助:
国家重点研发计划项目"非洲猪瘟专用消毒剂和虫媒防控技术研究"（2018YFC0840403）；中国农业科学院创新工程项目（FRI-06）

Optimization of Foaming Performance of Compound Quaternary Ammonium Salt Foam Disinfectant and Evaluation of Its Killing Effect on Viruses

LIU Huan^1,2, ZHANG Mengmeng², CUI Yifang³, LIU Jing², ZHANG Jingju², CHEN Dengjin⁴, LI Xiubo², LI Cun¹, XU Fei²

1. School of Animal Science and Animal Medicine, Tianjin Agricultural University, Tianjin 300384, China;
2. National Standard Laboratory of Feed Drugs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
3. Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100089, China;
4. College of Veterinary Medicine, China Agricultural University, Beijing 100193, China

Received:2022-05-26 Online:2022-12-05 Published:2022-12-01

摘要/Abstract

摘要： 【目的】研究复方季铵盐泡沫消毒剂的成泡性能，并评估该消毒剂对新城疫病毒（Newcastle disease virus，NDV）和猪瘟病毒（Classical swine fever virus，CSFV）的杀灭效果，以及不同作用浓度和时间对消毒效果的影响。【方法】通过响应面法对复方季铵盐泡沫消毒剂中苯扎溴铵、癸甲溴铵和癸基葡萄糖苷3种成分的含量进行优化；采用悬浮杀灭试验，以细胞感染方法，测定该消毒剂稀释度为1：100、1：200、1：400、1：500、1：600、1：800和1：1 000时分别作用5、10和15 min对NDV LaSota株的灭活率；采用悬液定量法，测定该消毒剂稀释度为1：200、1：300、1：400、1：500、1：600时分别作用3、5、7、10和15 min对CSFV Thiveral株的灭活率。【结果】复方季铵盐泡沫消毒剂中主要成分苯扎溴铵、癸甲溴铵和癸基葡萄糖苷含量配比分别为1.4%、3.8%和0.75%时，成泡时间达到83 min。该消毒剂在稀释度最高为1：400（以总含量计约为137.5 mg/L）时，最短作用时间5 min，对NDV LaSota株灭活率为100%；最短作用时间为3 min时，对CSFV Thiveral株灭活率达到100%；作用时间延长至15 min时，对2种病毒的灭活率均稳定保持在100%。复方季铵盐泡沫消毒剂的稀释度低于1：400时，对NDV LaSota株杀灭效果不佳；稀释度为1：500（以总含量计约为110 mg/L）、1：600（以总含量计约为91.67 mg/L）时，作用时间10 min，对CSFV Thiveral株灭活率达到100%，作用时间延长至15 min时，灭活率稳定保持在100%。【结论】复方季铵盐泡沫消毒剂中苯扎溴铵、癸甲溴铵和癸基葡萄糖苷含量为1.4%、3.8%和0.75%时成泡效果最优，该消毒剂对NDV和CSFV杀灭效果较好，为其在养殖场的规范使用提供参考与理论依据，也为畜禽养殖业场地环境消毒效果评价提供更多的可能性。

关键词: 泡沫消毒剂; 新城疫病毒(NDV); 猪瘟病毒(CSFV)

Abstract: 【Objective】 The purpose of the test was to study the foaming performance of compound quaternary ammonium salt foam disinfectant, and evaluate the killing effect of the disinfectant on Newcastle disease virus (NDV) and Classical swine fever virus (CSFV), as well as the effect on the disinfection effect at different action concentrations and different action time.【Method】 The specific contents of benzalkonium bromide, decylmethyl ammonium bromide and decyl glucoside in the compound quaternary ammonium salt foam disinfectant were optimized by the response surface method.Suspension killing test and cell infection method were used to determine the inactivation rate of the disinfectant against NDV LaSota strain at dilution of 1:100, 1:200, 1:400, 1:500, 1:600, 1:800 and 1:1000 for 5, 10 and 15 min respectively.Suspension quantitative method was used to determine the inactivation rate of the disinfectant against CSFV Thiveral strain when the dilution was 1:200, 1:300, 1:400, 1:500 and 1:600 for 3, 5, 7, 10 and 15 min respectively.【Result】 When the content ratio of benzalkonium bromide, decylmethyl ammonium bromide and decyl glucoside in the compound quaternary ammonium salt foam disinfectant was 1.4%, 3.8% and 0.75%, the foam forming time reached 83 min.When the compound quaternary ammonium salt foam disinfectant was diluted up to 1:400 (about 137.5 mg/L in terms of total content), the minimum action time was 5 min, and the inactivation rate of NDV LaSota strain was 100%, and the inactivation rate of CSFV Thiveral strain reached 100% at 3 min, when the action time was extended to 15 minutes, and the inactivation rate of both viruses was stable at 100%.When the dilution multiple of compound quaternary ammonium salt foam disinfectant was less than 1:400, the killing effect on NDV LaSota strain was not good.When the dilution multiple was 1:500 (about 110 mg/L in terms of total content), 1:600 (about 91.67 mg/L in terms of total content), the action time was 10 min, and the inactivation rate of CSFV Theiveral strain reached 100%, and when the action time was extended to 15 min, the inactivation rate was stable at 100%.【Conclusion】 When the content of benzalkonium bromide, decylmethyl ammonium bromide and decyl glucoside in the compound quaternary ammonium salt foam disinfectant was 1.4%, 3.8% and 0.75%, the foaming effect was the best.The disinfectant had a good killing effect on NDV and CSFV, which provided reference and theoretical basis for the establishment of its disinfection effect on NDV and CSFV and its standardized use in farms, and also provided more possibilities for the evaluation of the environmental disinfection effect of livestock and poultry breeding sites.

Key words: foam disinfectant; Newcastle disease virus (NDV); Classical swine fever virus (CSFV)

中图分类号:

S859.79^＋9.1

刘欢, 张蒙蒙, 崔一芳, 刘静, 张靖菊, 陈登金, 李秀波, 李存, 徐飞. 复方季铵盐泡沫消毒剂成泡性能的优化及对病毒的杀灭效果评价[J]. 中国畜牧兽医, 2022, 49(12): 4866-4877.

LIU Huan, ZHANG Mengmeng, CUI Yifang, LIU Jing, ZHANG Jingju, CHEN Dengjin, LI Xiubo, LI Cun, XU Fei. Optimization of Foaming Performance of Compound Quaternary Ammonium Salt Foam Disinfectant and Evaluation of Its Killing Effect on Viruses[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4866-4877.

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复方季铵盐泡沫消毒剂成泡性能的优化及对病毒的杀灭效果评价

Optimization of Foaming Performance of Compound Quaternary Ammonium Salt Foam Disinfectant and Evaluation of Its Killing Effect on Viruses

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