甘草提取物对绵羊皮下脂肪沉积与代谢相关酶活性的影响

›› 2013, Vol. 40 ›› Issue (11): 115-120.

甘草提取物对绵羊皮下脂肪沉积与代谢相关酶活性的影响

常彦飞¹, 罗海玲¹, 张玉伟¹, 李丽², 陈勇¹, 矫丽娟¹

1. 中国农业大学动物科学技术学院, 动物营养学国家重点实验室, 北京 100193;
2.宁夏农林科学院畜牧兽医研究所(有限公司), 宁夏银川 750002

收稿日期:2013-04-03 出版日期:2013-11-20 发布日期:2013-12-19
通讯作者: 罗海玲 E-mail:luohailing@cau.edu.cn
作者简介:常彦飞(1989-),女,河南人,硕士生,研究方向:反刍动物营养。
基金资助:
农业部行业科技(200903060);国家肉羊产业技术体系(CARS-39)。

Effects of Liquorice Extract on Subcutaneous Fat Deposition and Activities of Metabolism Related Enzymes in Sheep

CHANG Yan-fei¹, LUO Hai-ling¹, ZHANG Yu-wei¹, LI Li², CHEN Yong¹, JIAO Li-juan¹

1. State of Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;
2. Animal Husbandry and Veterinary Institute Co., Ltd., Ningxia Academy of Agro-forestry Sciences, Yinchuan 750002, China

Received:2013-04-03 Online:2013-11-20 Published:2013-12-19

摘要/Abstract

摘要： 作者旨在探讨添加不同水平甘草提取物(LE)对绵羊皮下脂肪沉积及代谢相关酶活性的影响。采用单因素试验设计,选用健康、断奶后20~30 d、初始体重相近的宁夏滩羊公羔50只,随机分为5组,每组10只,其中对照组饲喂基础日粮,不添加LE;试验Ⅰ~Ⅳ组分别在基础日粮中添加1000、2000、3000、4000 mg/kg LE。试验期120 d,试验结束后进行屠宰并测定滩羊皮下脂肪重、血清脂质代谢相关指标及皮下脂肪代谢相关酶活性。结果表明,与对照组相比,试验Ⅰ~Ⅳ组的皮下脂肪重分别降低了20.16%、14.40%、18.11%、20.16%;添加3000 mg/kg LE降低血清总胆固醇的效果最明显,LE对总甘油三酯(TG)和高密度脂蛋白(HDL-C)含量均无显著影响(P>0.05);LE显著降低皮下脂肪组织葡萄糖-6-磷酸脱氢酶(G6PDH)活性(P<0.05),其中添加3000 mg/kg LE降低G6PDH活性的效果最佳,对脂肪酸合成酶(FAS)、苹果酸脱氢酶(MDH)、异柠檬酸脱氢酶(ICDH)和6-磷酸脱氢酶(6PGDH)活性的影响均未达到显著水平(P>0.05)。提示,LE有降低血清总胆固醇和皮下脂肪重的趋势,其中添加3000 mg/kg LE的降脂作用最好,甘草提取物主要通过下调脂肪合成途径的关键酶活性来抑制绵羊皮下脂肪沉积,但甘草提取物调控滩羊皮下脂肪代谢关键酶的机制,尚需进一步研究。

关键词: 甘草提取物; 绵羊; 皮下脂肪代谢关键酶

Abstract: This study was conducted to investigate the effects of liquorice extract on subcutaneous fat deposition and the activities of metabolism related enzymes in sheep. In this single-factor experiment,50 healthy male lamb of Ningxia Tan sheep on 20 to 30 d after weaning with similar initial body weight were randomly divided into 5 groups and experiment Ⅰ to Ⅳ groups were fed with diets supplemented with 0,1000,2000,3000,4000 mg/kg basic ration liquorice extract (LE),respectively. After a trial period of 120 days,the sheep were slaughtered and the subcutaneous fat weight,serum lipid metabolism indices and subcutaneous fat metabolism related enzyme activity were tested. Results showed that compared with control group,the subcutaneous fat weight of trialⅠ to Ⅳ groups reduced by 20.16%,14.40%,18.11% and 20.16%,respectively. The effect of 3000 mg/kg LE was the best to decrease the serum total cholesterol(TC). But LE had no significant effect on total triglycerides (TG) and high density lipoprotein cholesterol (HDL-C) levels (P>0.05). The glucose 6-phosphate dehydrogenase (G6PDH) activity in subcutaneous adipose tissue was significantly suppressed by LE (P<0.05),and 3000 mg/kg LE was the best. But the influence of LE on the activities of fatty acid synthase (FAS),malic dehydrogenase(MDH),isocitrate dehydrogenase(ICDH) and 6-phosphate dehydrogenase (6PGDH) had no significant effect (P>0.05).Thus,LE tended to reduce the subcutaneous fat weight and serum total cholesterol, and 3000 mg/kg LE adding level had the best lipid-lowering effect,and LE reduced fat deposition mainly by down regulating the key enzymes in fat synthesis approach,however,the specific regulation mechanism of LE on subcutaneous fat metabolism key enzymes in sheep still needed to further study.

Key words: liquorice extract; sheep; subcutaneous fat metabolism

中图分类号:

S816.7

常彦飞, 罗海玲, 张玉伟, 李丽, 陈勇, 矫丽娟. 甘草提取物对绵羊皮下脂肪沉积与代谢相关酶活性的影响[J]. , 2013, 40(11): 115-120.

CHANG Yan-fei, LUO Hai-ling, ZHANG Yu-wei, LI Li, CHEN Yong, JIAO Li-juan. Effects of Liquorice Extract on Subcutaneous Fat Deposition and Activities of Metabolism Related Enzymes in Sheep[J]. , 2013, 40(11): 115-120.

参考文献

1 尤翠兰,苏佩清,周晓霞.黄芩茎叶总黄酮调血脂作用及其机制的研究[J].中国中药志,2008,33(9):1064~1067.
2 冯毓秀,林寿全.甘草的本草考证及研究概况[J].时珍国医国药,1993,4(2):41~44.
3 田维熙,董妍,权晖,等.不同生长期蛋鸡的体脂水平和肝脏脂肪酸合成酶活性的关系[J].生物化学杂志,1996,12(2):234~236.
4 张波,官月平,田庆来.乌拉尔甘草中黄酮类化学成分的研究进展[J].中成药,2006,28(9):1362~1365.
5 李莉,朱晓彤,束刚,等.日粮中添加山楂叶总黄酮对黄羽肉鸡脂肪代谢的影响[J].江西农业大学学报,2009,31(4):610~615.
6 单安山,徐奇友.动物脂肪代谢与调控[J].东北农业大学学报,2004,35(2):216~221.
7 罗建学,李春风,初晓辉,等.脂肪酸合成酶基因的研究进展[J].中国畜牧兽医,2011,38(6):118~123.
8 金邦荃,陈杰.动物脂肪代谢与控制[J].动物科学与动物医学,2001,18(6):9~11.
9 姚飞.苏尼特肥羔脂肪代谢酶活性的发育变化及其对IMF沉积的影响[D].呼和浩特:内蒙古农业大学,2008.
10 夏蕾,张志宏,左金国.沙棘提取物对猪脂肪中部分脂肪代谢相关基因表达的影响[J].营养学报,2009,31(2):177~180.
11 高发奎.甘草废渣的开发利用技术[J].甘肃环境研究与检测,2002,15(1):27~28.
12 焦岩,王振宇.大果沙棘果渣黄酮降血脂与抗氧化作用[J].营养学报,2009,31(5):516~519.
13 谢伟华,孙超,刘淑敏.山楂黄酮对高脂血模型小鼠血脂及生脂基因转录表达的影响[J].中国中药杂志,2009,2(34):224~229.
14 颜新春,汪以真,许梓荣.动物脂肪酸合成酶(FAS)基因表达的调控[J].动物营养学报,2002,14(2):1~4.
15 Aoki F,Honda S,Kishida H,et al. Suppression by licorice flavonoids of abdominal fat accumulation and body weight gain in high-fat diet-induced obese C57BL/6J mice. Biosci Biotech Bioch,2007,71(1):206~214.
16 Bauman D E,Brown R E,Davis C L. Pathways of fatty acid synthesis and reducing equivalent generation in mammary gland of rat,sow,and cow. Arch Biochem Biophys,1970,140(1):237~244.
17 Cahaner A,Nitsan Z,Nir I. Weight and fat content of adipose and nonadipose tissues in broilers selected for or against abdominal adipose tissue. Poultry Sci,1986,65(2):215~222.
18 Honda K,Kamisoyama H,Tominaga Y,et al. The molecular mechanism underlying the reduction in abdominal fat accumulation by licorice flavonoid oil in high fat diet-induced obese rats. J Anim Sci,2009,80:562~569.
19 Martin R J,Wilson L L,Cowan R L,et al. Effects of fasting and diet on enzyme profiles in ovine liver and adipose tissue. J Anim Sci,1973,36:101~106.
20 McNeel R L,Ding S T,Smith E O,et al. Effect of feed restriction on adipose tissue transcript concentrations in genetically lean and obese pigs. J Anim Sci,2000,78:934~942.
21 Moibi J A,Ekpe E D,Christopherson R J. Acetyl-CoA carboxylase and fatty acid synthase activity and immunodetectable protein in adipose tissues of ruminants: Effect of temperature and feeding level. J Anim Sci,2000,78:2383~2392.
22 Nakagawa K,Kishida H,Arai N,et al. Licorice flavonoids suppress abdominal fat accumulation and increase in blood glucose level in obese diabetic KK-A^y mice. Biol Pharm Bull,2004,27(11):1775~1778.
23 Nguyen P,Leray V,Diez M,et al. Liver lipid metabolism.J Anim Physiol An N,2008,92(3):272~283.
24 Suagee J K,Corl B A,Crisman M V,et al. De novo fatty acid synthesis and NADPH generation in equine adipose and liver tissue. Comp Biochem Phys B,2010,155(3):322~326.
25 Tominaga Y,Nakagawa K,Mae T,et al. Licorice flavonoid oil reduces total body fat and visceral fat in overweight subjects:A randomized,double-blind,placebo-controlled study. Obes Res Clin Pract,2009(3):169~178.
26 Tsujita T,Ninomiya H,Okuda H. P-nitrophenyl butyrate hydrolyzing activity of hormone-sensitive lipase from bovine adipose tissue. J Lipid Res,1989,30:997~1004.
27 Xiao C W,Mei J,Wood C M. Effect of soy proteins and isoflavones on lipid metabolism and involved gene expression. Front Biosci,2008,13:2660~2673.
28 Zhang Q,Ye M. Chemical analysis of Chinese herbal medicine Gan-Cao (licorice). J Chromatogr A,2009,1216(11):1954~1969.

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