16S rRNA甲基化酶在鸡肠道大肠杆菌中传播方式的分析

›› 2013, Vol. 40 ›› Issue (10): 216-220.

16S rRNA甲基化酶在鸡肠道大肠杆菌中传播方式的分析

丁小丽, 陈琳, 黄东璋, 刘俊栋, 魏冬霞, 管远红, 李巨银, 周思思, 陈新秀

江苏农牧科技职业学院, 江苏泰州 225300

修回日期:2013-06-19 出版日期:2013-10-20 发布日期:2013-12-19
通讯作者: 陈琳 E-mail:chenlin20086666@126.com
作者简介:丁小丽(1979-),女,甘肃人,硕士,讲师,研究方向:基础兽医。
基金资助:
江苏省自然科学基金(BK2008587);江苏畜牧兽医职业技术学院重点项目(ZD1202)。

Analysis of Transfer Modes of 16S rRNA Methylase in Chicken Intestinal E.coli

DING Xiao-li, CHEN Lin, HUANG Dong-zhang, LIU Jun-dong, WEI Dong-xia, GUAN Yuan-hong, LI Ju-yin, ZHOU Si-si, CHEN Xin-xiu

Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, China

Revised:2013-06-19 Online:2013-10-20 Published:2013-12-19

摘要/Abstract

摘要： 试验旨在分析16S rRNA甲基化酶耐药基因在鸡肠道大肠杆菌中的传播方式。采用质粒接合试验研究rmtB和armA基因的水平传播方式,计算接合频率,并用微量稀释法分析原菌株及接合子对抗菌药物的敏感性。结果显示,5株rmtB阳性菌均可通过接合试验将耐药基因传递给受体菌E.coli C600,每个受体细胞的接合频率从4.3×10^-10至5.5×10^-6不等;而armA阳性菌无法通过接合试验将耐药基因传递给受体菌。研究结果表明,rmtB基因在鸡肠道大肠杆菌中以水平传播方式为主,而armA基因在鸡肠道大肠杆菌中的传播方式有待于进一步研究。

关键词: 16S rRNA甲基化酶; 质粒; 水平传播; 耐药

Abstract: To study the transfer mode of rmtB and armA in chicken intestinal E.coli,plasmid conjugation experiment was carried out to study the horizontal transfer mode of rmtB and armA genes, and conjugative transfer frequencies were calculated. Broth microdilution method was applied to test susceptibility of 16S rRNA emthylase-positive isolates and their transconjugants to antimicrobial agents. As a result, all the rmtB genes located in five rmtB-positive isolates were easily transferred to recipients of E.coli C600. Conjugative transfer frequencies varied from 4.30×10^-10 to 5.5×10^-6 per recipient cell, and the armA gene located in one armA-positive isolate was not easily transferred to the recipient of E.coli C600. The results suggested that horizontal transfer was responsible for the dissemination of rmtB gene in chicken intestinal E.coli, but not for armA.

Key words: 16S rRNA methylase; plasmid; horizontal transfer; resistance

中图分类号:

S859.7

丁小丽, 陈琳, 黄东璋, 刘俊栋, 魏冬霞, 管远红, 李巨银, 周思思, 陈新秀. 16S rRNA甲基化酶在鸡肠道大肠杆菌中传播方式的分析[J]. , 2013, 40(10): 216-220.

DING Xiao-li, CHEN Lin, HUANG Dong-zhang, LIU Jun-dong, WEI Dong-xia, GUAN Yuan-hong, LI Ju-yin, ZHOU Si-si, CHEN Xin-xiu. Analysis of Transfer Modes of 16S rRNA Methylase in Chicken Intestinal E.coli [J]. , 2013, 40(10): 216-220.

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