不同周龄蛋鸡卵巢机能及氧化还原状态的变化研究

摘要/Abstract

摘要： 本试验旨在研究不同周龄蛋鸡卵巢机能及氧化还原状态的变化规律。选择17周龄京红蛋鸡48只、78周龄京红蛋鸡24只,2只1笼为1个重复,分别饲养至36、48、97周龄采样。结果发现,随着周龄增大,蛋鸡卵巢机能衰退,表现为卵巢内等级卵泡、小黄卵泡、初级卵泡和次级卵泡数量显著减少(P<0.05),小黄卵泡、大白卵泡和次级卵泡闭锁率显著增加(P<0.05);血清中黄体生成素和孕酮的含量均呈现降低趋势,但差异不显著(P>0.05);随着周龄增大,蛋鸡机体氧化损伤加剧,表现为肝脏、卵巢中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性均显著降低(P<0.05),丙二醛(MDA)水平显著升高(P<0.05)。结果表明,蛋鸡卵巢机能衰退可能与体内(肝脏、卵巢和血清)氧化损伤加重有关。

关键词: 不同周龄; 蛋鸡; 卵巢机能; 氧化损伤; 闭锁卵泡

Abstract: This experiment was conducted to evaluate the effect of age on ovary function and oxidative stress in laying hens.48 Jinghong hens of 17 weeks of age were selected and fed with the basal diet until 36 and 48 weeks of age.24 Jinghong hens of 78 weeks of age fed with the basal diets until 97 weeks of age. The results showed as follows: compared with the young hens,numbers of hierarchical yellow follicles,small yellow follicles,primary follicles and secondary follicles were decreased significantly (P<0.05),whereas the follicular atresia rate of smaller yellow follicles,large white follicles and secondary follicles were increased significantly (P<0.05).The content of LH and progesterone in serum were decreased as age growing, but there were no significant differences at 36, 48 and 97 weeks of age (P>0.05).The content of SOD and GSH-Px in liver and ovarian were significantly decreased as age growing (P<0.05),whereas the content of MAD increased significantly (P<0.05).These results indicated that ovary function decline might be related with oxidative stress of liver,ovary and serum.

Key words: weeks of age; laying hens; ovary function; oxidative stress; follicular atresia

中图分类号:

S831.1

姜礼文, 冯京海, 张敏红, 陈燕. 不同周龄蛋鸡卵巢机能及氧化还原状态的变化研究[J]. , 2013, 40(10): 165-169.

JIANG Li-wen, FENG Jing-hai, ZHANG Min-hong, CHEN Yan . Influence of Age on Ovary Function and Oxidative Stress in Laying Hens[J]. , 2013, 40(10): 165-169.

参考文献

1 李莹辉,汪琳仙,杨传任.PMSG及GnRH类似物对鸡卵泡闭锁的调节作用[J].畜牧兽医学报,1999(1):12~19.
2 杨保栓,张晓根,汪德刚,等.高产老龄蛋鸡输卵管综合症病理研究与治疗试验[J].河南农业科学,2003(7):60~62.
3 杨晓岚,张敏红,冯京海,等.高温诱导蛋鸡氧化损伤及对卵泡发育的影响[J].中国农业科学,2012,45(16):3391~3398.
4 范燕燕,林守清,何仲,等.卵巢功能衰退过程中血清激素和卵巢大小的改变[J].中国妇幼健康研究,2012,23(2):159~162.
5 耿建军.不同日龄蛋鸡卵泡发育、血清FSH和E2值变化规律以及转化生长因子β在卵巢和输卵管表达规律的研究[D].太谷:山西农业大学,2003.
6 Bahr J M,Wang S C,Huang M Y,et al.Steroid concentrations in isolated theca and granulosa layers of preovulatory follicles during the ovulatory cycle of the domestic hen[J].Biology of Reproduction,1983,29(2):326~334.
7 Carbone M C,Tatone C,Delle Monache S,et al.Antioxidant enzymatic defences in human follicular fluid:Characterization and age-dependent changes[J].Molecular Human Reproduction,2003,9(11):639~643.
8 De Bruin J P,Dorland M,Spek E R,et al.Age-related changes in the ultrastructure of the resting follicle pool in human ovaries[J].Biology of Reproduction,2004,70(2):419~424.
9 Holmes D J,Thomson S L,Wu J,et al.Reproductive aging in female birds[J].Experimental Gerontology,2003a,38(7):751~756.
10 Holmes D J,Ottinger M A.Birds as long-lived animal models for the study of aging[J].Experimental Gerontology,2003b,38(11):1365~1375.
11 Johnson A L,Van Tienhoven A.Plasma concentrations of six steroids and LH during the ovulatory cycle of the hen,Gallus domesticus[J].Biology of Reproduction,1980,23(2):386~393.
12 Johnson P A,Dickerman R W,Bahr J M.Decreased granulosa cell luteinizing hormone sensitivity and altered thecal estradiol concentration in the aged hen,Gallus domesticus[J].Biology of Reproduction,1986,35(3):641~646.
13 Lillpers K,Wilhelmson M.Age-dependent changes in oviposition pattern and egg production traits in the domestic hen[J].Poultry Science,1993,72(11):2005~2011.
14 Liu H K,Bacon W L.Effect of chronic progesterone injection on egg production in Japanese quail[J].Poultry Science,2004,83(12):2051~2058.
15 Moudgal R P,Razdan M N.Induction of ovulation in vitro in the hen:Dependency of the response to LH on age and rate of lay[J].Journal of Endocrinology,1985,106(1):67~69.
16 Palmer S S,Bahr J M.Follicle stimulating hormone increases serum oestradial-17β concentrations,number of growing follicles and yolk deposition in aging hens (Gallus gallus domesticus) with decreased egg production[J].British Poultry Science,1992,33(2):403~414.
17 Porter T E,Hargis B M,Silsby J L,et al.Characterization of dissimilar steroid productions by granulosa,theca interna and theca externa cells during follicular maturation in the turkey (Meleagris gallopavo)[J].General and Comparative Endocrinology,1991,84(1):1~8.
18 Senthilkumaran C,Peterson S,Taylor M,et al.Use of a vascular access port for the measurement of pulsatile luteinizing hormone in old broiler breeders[J].Poultry Science,2006,85(9):1632~1640.
19 Tamura H,Takasaki A,Miwa I,et al.Oxidative stress impairs oocyte quality and melatonin protects oocytes from free radical damage and improves fertilization rate[J].Journal of Pineal Research,2008,44(3):280~287.
20 Tarin J J.Potential effects of age-associated oxidative stress on mammalian oocytes/embryos[J].Molecular Human Reproduction,1996,2(10):717~724.
21 Tatone C,Carbone M C,Falone S,et al.Age-dependent changes in the expression of superoxide dismutases and catalase are associated with ultrastructural modifications in human granulosa cells[J].Molecular Human Reproduction,2006,12(11):655~660.
22 Tsai-Turton M,Luderer U.Opposing effects of glutathione depletion and follicle-stimulating hormone on reactive oxygen species and apoptosis in cultured preovulatory rat follicles[J].Endocrinology,2006,147(3):1224~1236.
23 Waddington D,Perry M M,Gilbert A B,et al.Follicular growth and atresia in the ovaries of hens (Gallus domesticus) with diminished egg production rates[J].Journal of Reproduction and Fertility,1985,74(2):399~405.
24 Zakaria A H,Miyaki T,Imai K.The effect of aging on the ovarian follicular growth in laying hens[J].Poultry Science,1983,62(4):670~674.
25 Zakaria A H.Ovarian follicular development in young and old laying hens[J].Archiv Für Geflügelkunde,1999,63.

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