木质纤维素对青年后备蛋鸡胃肠发育和肠道功能的影响

doi:10.16431/j.cnki.1671-7236.2021.09.013

摘要/Abstract

摘要： 本试验旨在研究木质纤维素作为不溶性纤维来源对青年后备蛋鸡胃肠发育和肠道功能的影响。将375只28日龄的海兰褐青年后备蛋鸡随机分成3组，每组5个重复，每个重复25只，分别饲喂添加0（对照组）、8（0.8%木质纤维素组）和10 g/kg木质纤维素（1.0%木质纤维素组）的玉米-豆粕型饲粮。试验期为56 d，试验结束时，计算平均日增重、平均日采食量和料重比；测定各组鸡的器官指数、回肠消化酶活性、紧密连接相关基因表达和肠形态学变化。结果显示，与对照组相比，添加木质纤维素对青年后备蛋鸡平均日增重、平均日采食量和料重比均无显著影响（P>0.05），但可显著增加青年后备蛋鸡肌胃重量和肌胃器官指数（P<0.05）。饲喂木质纤维素后，鸡回肠绒毛宽度和高度均显著高于对照组（P<0.05），且1.0%木质纤维素组肠绒毛高度显著高于0.8%木质纤维素组（P<0.05）；0.8%木质纤维素组回肠α-淀粉酶和胰蛋白酶活性及紧密连接蛋白1（ZO-1）和ZO-2 mRNA表达水平显著高于对照组（P<0.05）。综上，添加8 g/kg木质纤维素可刺激蛋鸡肌胃发育、促进肠道消化酶活性、提高肠道形态结构的完整性，维护青年后备蛋鸡肠道健康。

关键词: 木质纤维素; 青年后备蛋鸡; 胃肠发育; 肠道健康

Abstract: This experiment was conducted to study the effects of lignocellulose as an insoluble fiber source on gastrointestinal development and gut function of young layers. A total of 375 28-day-old Hy-line Brown commercial young layers were randomly divided into 3 groups with 5 replicates, and each replicate had 25 chickens. Each group was fed corn-soybean meal diet supplemented with 0 (control group), 8 (0.8% lignocellulose group), and 10 g/kg lignocellulose (1.0% lignocellulose group), respectively. The feeding experiment lasted for 56 days. At the ending of the experiment, the average daily gain, average daily feed intake, and feed to gain ratio were calculated. Additionally, the organ index, ileal digestive enzyme activities, tight junction-related gene expression, and morphological changes were measured. The results showed that, compared with the control group, the addition of lignocellulose had no significant effect on the average daily gain, average daily feed intake, and feed to gain ratio(P>0.05), but significantly increased the weight of muscular stomach and muscular stomach organ index of young layers (P<0.05). Adding lignocellulose significantly increased the villus width and height of chickens (P<0.05), and the villus height of the 1.0% lignocellulose group was higher than that of the 0.8% lignocellulose group (P<0.05). The activities of ileum α-amylase and trypsin and the mRNA levels of tight junction ZO-1 and ZO-2 in the 0.8% lignocellulose group were higher than those in the control group (P<0.05). In conclusion, the addition of 8 g/kg lignocellulose could stimulate the development of muscular stomach, increase intestinal digestive enzyme activities, and improve the integrity of intestinal morphology that maintaining gut health of young layers.

Key words: lignocellulose; young layer; gastrointestinal development; gut health

中图分类号:

S816.7

朱丽慧, 吕文纬, 孔德富, 杨长锁. 木质纤维素对青年后备蛋鸡胃肠发育和肠道功能的影响[J]. 中国畜牧兽医, 2021, 48(9): 3223-3231.

ZHU Lihui, LYU Wenwei, KONG Defu, YANG Changsuo. Effect of Lignocellulose on Gastrointestinal Development and Intestinal Function of Young Laying Hens[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3223-3231.

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