白藜芦醇对玉米赤霉烯酮致小鼠肝脏氧化损伤及炎症的保护作用

doi:10.16431/j.cnki.1671-7236.2021.11.035

摘要/Abstract

摘要： 为探究白藜芦醇（RSV）对玉米赤霉烯酮（ZEA）中毒小鼠肝脏损伤是否具有保护作用，试验将40只清洁级雄性BALB/c小鼠随机分为5组：对照组（生理盐水）、ZEA组（40 mg/kg）、不同浓度的RSV （50、100或200 mg/kg）与ZEA （40 mg/kg）共处理组，每组8只，各组均灌胃给药，试验期12 d。试验结束后采集小鼠肝脏样品，计算肝脏系数，采用苏木素-伊红（HE）染色和免疫组织化学染色法观察各组肝脏病理变化；检测肝脏NF-κB蛋白表达；比色法检测肝脏组织中丙二醛（MDA）含量、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性及血清中谷丙转氨酶（ALT）、谷草转氨酶（AST）和乳酸脱氢酶（LDH）活性，ELISA法检测血清中白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）和IL-1β含量。结果显示，与对照组相比，ZEA组小鼠肝组织发生明显病理学变化；与ZEA组相比，不同浓度RSV与ZEA共处理组小鼠肝脏组织病理学变化都有所减轻。免疫组织化学结果显示，与对照组相比，ZEA组肝脏NF-κB蛋白表达增多；与ZEA组相比，不同浓度的RSV与ZEA共处理组肝脏NF-κB蛋白表达均有下降。与对照组相比，ZEA组肝脏系数和肝脏组织MDA含量极显著升高（P<0.01），肝脏组织SOD和CAT活性显著或极显著降低（P<0.05；P<0.01），血清中AST、ALT和LDH活性，IL-6、TNF-α和IL-1β含量均极显著升高（P<0.01）。与ZEA组相比，不同浓度RSV与ZEA共处理组肝脏组织CAT活性显著或极显著升高（P<0.05；P<0.01），血清中AST、ALT和LDH活性及IL-6和IL-1β含量显著或极显著降低（P<0.05；P<0.01）；50 mg/kg RSV与ZEA共处理组肝脏组织SOD活性和血清中TNF-α含量分别显著升高（P<0.05）和极显著降低（P<0.01）；100和200 mg/kg RSV与ZEA共处理组肝脏系数和肝脏组织MDA含量显著或极显著降低（P<0.05；P<0.01）。结果表明，ZEA对小鼠肝脏有严重的氧化及炎症损伤作用，RSV对ZEA中毒的肝损伤具有一定保护作用，尤其以100 mg/kg RSV保护效果最佳。

关键词: 白藜芦醇; 玉米赤霉烯酮; 氧化损伤; 炎症; 肝脏

Abstract: In order to explore whether resveratrol (RSV) had a protective effect on the liver injury of mice induced by zearalenone (ZEA), 40 clean grade male BALB/c mice were randomly divided into 5 groups:Control group (normal saline), ZEA group (40 mg/kg), different concentrations of RSV (50, 100 or 200 mg/kg) and ZEA (40 mg kg) co-treatment groups, with 8 mice in each group. Each group was administered by gavage for 12 days. After the experiment, the liver samples of mice were collected, the liver coefficient was calculated, the liver pathological changes were observed by HE staining and immunohistochemical staining, and the liver NF-κB was detected. The content of malondialdehyde (MDA), the activities of superoxide dismutase (SOD) and catalase (CAT) in liver tissue were detected by colorimetry. The activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) in serum were detected by colorimetry. The levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and IL-1β in serum were detected by ELISA method. The results showed that compared with control group, the liver tissue of ZEA group had obvious pathological changes. Compared with ZEA group, the liver histopathological changes of mice treated with different concentrations of RSV and ZEA were reduced. Immunohistochemical results showed that compared with control group, liver NF-κB protein expression was increased. Compared with ZEA group, liver NF-κB protein expression of different concentrations of RSV and ZEA co-treatment groups were decreased. Compared with control group, the liver coefficient and the content of MDA in liver tissue in ZEA group were extremely significantly increased (P<0.01), the activities of SOD and CAT in liver tissue were significantly or extremely significantly decreased (P<0.05; P<0.01), the activities of AST, ALT and LDH, and the contents of IL-6 and TNF-α and IL-1β in serum were extremely significantly increased (P<0.01). Compared with ZEA group, CAT activity in liver tissue of different concentrations of RSV and ZEA co-treatment groups were significantly or extremely significantly increased (P<0.05; P<0.01); Activities of AST, ALT and LDH and the contents of IL-6 and IL-1β in serum were significantly or extremely significantly decreased (P<0.05; P<0.01); SOD activity in liver tissue and the content of TNF-α in serum of 50 mg/kg RSV and ZEA co-treatment group were significantly increased (P<0.05) and extremely significantly decreased (P<0.01), respectively; The liver coefficient and MDA content in liver tissue were significantly or extremely significantly decreased in 100 and 200 mg/kg RSV and ZEA co-treatment groups (P<0.05; P<0.01). The results showed that ZEA had serious oxidative and inflammatory effects on the liver, and RSV had a certain protective effect on the liver injury caused by ZEA poisoning, 100 mg/kg RSV had the best protect effect.

Key words: resveratrol; zearalenone; oxidative damage; inflammation; liver

中图分类号:

S853.74

朱根生, 夏苏干, 佘进进, 白玉妮, 邹辉, 顾建红, 袁燕, 刘学忠, 刘宗平, 卞建春. 白藜芦醇对玉米赤霉烯酮致小鼠肝脏氧化损伤及炎症的保护作用[J]. 中国畜牧兽医, 2021, 48(11): 4254-4261.

ZHU Gensheng, XIA Sugan, SHE Jinjin, BAI Yuni, ZOU Hui, GU Jianhong, YUAN Yan, LIU Xuezhong, LIU Zongping, BIAN Jianchun. Protective Effects of Resveratrol on Zearalenone-induced Liver Oxidative Damage and Inflammation in Mice[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 4254-4261.

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