绞股蓝多糖对D-半乳糖致衰老小鼠肝脏抗氧化能力的影响

doi:10.16431/j.cnki.1671-7236.2022.02.007

摘要/Abstract

摘要： 【目的】探究绞股蓝多糖(Gynostemma pentaphyllum polysaccharides, GPP)对D-半乳糖(D-galactose, D-gal)致衰老小鼠肝脏抗氧化能力的影响。【方法】 60只4周龄SPF级昆明小鼠随机分为6组(雌雄各半): 空白对照组(Control)、模型组(D-gal)、阳性对照组(Vc)及绞股蓝多糖低(50 mg/kg)、中(100 mg/kg)、高(200 mg/kg)剂量组(GPP-L、GPP-M和GPP-H), 每组10只。采用生物化学、酶联免疫吸附及组织学技术等方法分别对各试验组小鼠的体重及增重变化、肝脏指数、血清总抗氧化能力(T-AOC)水平、肝脏超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)活性、活性氧(ROS)、丙二醛(MDA)含量及肝脏组织形态和肝索面积比进行测定分析。【结果】各组小鼠体重均呈增长趋势, 增重均呈下降趋势, 其中GPP-M组小鼠体重低于其他各组, 增重最少; GPP各剂量组肝脏指数、血清T-AOC水平均高于D-gal组; GPP各剂量组的小鼠肝脏组织SOD水平与D-gal组相比均无显著差异(P>0.05)。GSH-Px、CAT活性水平与D-gal组相比均有所增强, 其中GPP-M组小鼠肝脏中GSH-Px活性显著高于D-gal组(P<0.05);GPP-L组小鼠肝脏中CAT活性显著高于D-gal组(P < 0.05), ROS、MDA含量均显著低于D-gal组(P<0.05)。HE染色发现, D-gal组胞浆嗜酸性增强, 肝细胞之间连接松散, 肝血窦扩张明显, 而GPP各组肝脏组织结构均有所改善, GPP-M与GPP-H组改善较明显; GPP-H组肝脏肝索面积占比与空白对照组和阳性对照组相当, GPP-M和GPP-H组肝脏肝索面积占比与GPP-L组相比显著增加(P<0.05)。【结论】 GPP可以有效提高D-gal致衰老小鼠肝脏的抗氧化能力, 从而达到延缓衰老的作用, 其中GPP-M(100 mg/kg)组对D-gal致衰老小鼠肝脏抗氧化效果明显。

关键词: 绞股蓝多糖(GPP); 肝脏; D-半乳糖(D-gal)致衰老; 抗氧化

Abstract: 【Objective】 The purpose of this study was to investigate the effect of Gynostemma pentaphyllum polysaccharides (GPP) on the antioxidant capacity of liver in aging mice induced by D-galactose (D-gal).【Method】 Sixty 4-week-old SPF Kunming mice were randomly divided into 6 groups (half male and half female): Blank control group (Control), model group (D-gal), positive control group (Vc), low (50 mg/kg), medium (100 mg/kg) and high (200 mg/kg) dose groups of GPP (GPP-L, GPP-M and GPP-H), with 10 mice in each group.Biochemical, enzyme-linked immunosorbent assay and histological techniques were used to determine the weight change, liver index, T-AOC levels in serum, SOD, GSH-Px and CAT activities, ROS and MDA contents in liver, as well as the liver tissue morphology and liver cord area ratio.【Result】 The weight of mice in each group was an increasing trend, and the weight gain was a decreasing trend, the weight of mice in GPP-M group was lower than that of the other groups, and the weight gain was the least.The liver index and serum T-AOC activity in each dose group of GPP were higher than that in D-gal group.The SOD activity in each dose group of GPP was no significant difference with D-gal group (P > 0.05).The activities of GSH-Px and CAT were increased compared with D-gal group, the GSH-Px activity in GPP-M group and the CAT activity in GPP-L group were significantly higher than that in D-gal group (P < 0.05), and the contents of ROS and MDA in GPP-L group were significantly decreased than that in D-gal group (P < 0.05).HE staining revealed that the cytoplasmic eosinophilia of D-gal group was enhanced, the connection between hepatocytes was loose, and the hepatic sinusoids were dilated significantly, while the liver tissue structure in GPP group was improved, especially GPP-M and GPP-H groups.The proportion of hepatic cord area in GPP-H group was equivalent to that in blank and positive control groups, and the proportion of hepatic cord area in GPP-M and GPP-H groups were significantly higher than that in GPP-L group (P < 0.05).【Conclusion】 GPP could effectively improve the antioxidant capacity of aging mice induced by D-gal and delay the aging, and 100 mg/kg GPP had a significant antioxidant effect on liver of aging mice induced by D-gal.

Key words: Gynostemma pentaphyllum polysaccharide (GPP); liver; D-galactose (D-gal) induced aging; antioxidant

中图分类号:

S852.1

张润祥, 白玉婷, 张颖蕾, 刘可可, 王欣雨, 董胤余, 王晓丽. 绞股蓝多糖对D-半乳糖致衰老小鼠肝脏抗氧化能力的影响[J]. 中国畜牧兽医, 2022, 49(2): 462-470.

ZHANG Runxiang, BAI Yuting, ZHANG Yinglei, LIU Keke, WANG Xinyu, DONG Yinyu, WANG Xiaoli. Effect of Gynostemma pentaphyllum Polysaccharide on Antioxidative Ability of Liver in Aging Mice Induced by D-galactose[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 462-470.

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