›› 2012, Vol. 39 ›› Issue (4): 76-78.

• 生物技术 • 上一篇    下一篇

大肠杆菌毒力因子agn43与生物被膜表型相关性研究

陈朝喜, 杨金福, 陈伟明   

  1. 西南民族大学生命科学与技术学院,四川成都 610041
  • 收稿日期:1900-01-01 修回日期:2011-11-09 出版日期:2012-04-20 发布日期:2012-04-20

Studies on Correlation between E.coli Virulence Factor agn43 and Biofilm-forming Ability

CHEN Chao-xi, YANG Jin-fu, CHEN Wei-ming   

  1. College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China
  • Received:1900-01-01 Revised:2011-11-09 Online:2012-04-20 Published:2012-04-20

摘要: 分别采用改良结晶紫半定量方法和常规PCR方法对180株宠物源性大肠杆菌进行生物被膜形成能力定量研究和相变抗原agn43检测,以分析大肠杆菌毒力因子agn43与生物被膜表型相关性。试验结果表明,30.1%(55/180)的菌株能够检测到agn43基因,96.4%(53/55)agn43阳性菌株具有不同程度的成膜能力,与无成膜能力组差异极显著(P<0.01)。

关键词: 大肠杆菌; 生物被膜; 毒力因子; agn43

Abstract: To elucidate the correlation between biofilm-forming ability and virulence factors, improved crystal violet semi-quantitative method and common PCR were used for biofilm-forming ability quantitative analysis and phase variation antigen 43 detection in 180 pet-brone E.coli respectively; the results revealed that 30.1%(55/180) of the strains detected agn43 and 96.4%(53/55)of the agn43 positive strains performed biofilm-forming ability, showing significant difference from non-biofilm forming group(P<0.01).

Key words: E.coli; biofilm; virulence factor; agn43

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