鸡源携带多黏菌素耐药基因mcr-1大肠杆菌耐药性与毒力基因相关性分析

doi:10.16431/j.cnki.1671-7236.2025.03.040

中国畜牧兽医 ›› 2025, Vol. 52 ›› Issue (3): 1393-1404.doi: 10.16431/j.cnki.1671-7236.2025.03.040

• 基础兽医 • 上一篇

鸡源携带多黏菌素耐药基因mcr-1大肠杆菌耐药性与毒力基因相关性分析

戴婷婷^1,2, 陈海玉^1,2, 段楚楚^1,2, 刘荣昌³, 李宇蓉², 严淑涵², 陈梦诗², 刘露薇², 包银莉^2,4, 程艳青^2,4, 林炜明^2,4, 黄翠琴^2,4, 郑新添^2,4

1. 福建农林大学动物科学学院, 福州 350002;
2. 龙岩学院生命科学学院, 龙岩 364012;
3. 福建省农业科学院畜牧兽医研究所, 福建省禽病防治重点实验室, 福州 350003;
4. 动物源性人兽共患病防控福建省高校工程研究中心, 龙岩 364012

收稿日期:2024-08-13 发布日期:2025-02-22
通讯作者: 黄翠琴, 郑新添
作者简介:戴婷婷，E-mail:2214251744@qq.com。
基金资助:
国家自然科学基金青年基金项目(32302883);动物源性人兽共患病防控福建省高校工程研究中心开放基金项目(2022K010);福建省中青年教师教育科研项目(JAT210434);新罗区产学研科技联合创新项目(2022XLXYZ011);龙岩学院预防兽医学与生物技术福建省高等学校重点实验室开放基金项目(2022KF03)

Correlation Analysis of Drug Resistance and Virulence Genes of Escherichia coli with Polycolistin Resistance Gene mcr-1 from Chickens

DAI Tingting^1,2, CHEN Haiyu^1,2, DUAN Chuchu^1,2, LIU Rongchang³, LI Yurong², YAN Shuhan², CHEN Mengshi², LIU Luwei², BAO Yinli^2,4, CHENG Yanqing^2,4, LIN Weiming^2,4, HUANG Cuiqin^2,4, ZHENG Xintian^2,4

1. College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
2. School of Life Science, Longyan University, Longyan 364012, China;
3. Fujian Key Laboratory of Avian Disease Control, Institute of Animal Science and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China;
4. Engineering Research Center of Zoonotic Diseases Prevention and Control, Fujian Universities, Longyan 364012, China

Received:2024-08-13 Published:2025-02-22

摘要/Abstract

摘要： 【目的】分析福建省鸡源携带多黏菌素耐药基因mcr-1大肠杆菌的耐药性与毒力基因之间的相关性，并对mcr-1耐药基因质粒转移特性进行研究。【方法】以临床分离的87株鸡源携带mcr-1基因大肠杆菌为研究对象，通过微量肉汤稀释法检测其对6大类11种抗菌药物的敏感性，利用PCR方法检测分离株耐药基因、毒力基因和质粒类型，分析mcr-1基因阳性菌耐药表型和耐药基因以及耐药基因和毒力基因之间的相关性；利用质粒接合试验，探究mcr-1基因的转移情况。【结果】药敏试验结果显示，96.55%(85/87)分离株为多重耐药菌，其中对四环素类的耐药最严重，多西环素和四环素耐药率分别为88.51%(77/87)和82.76%(72/87)。分离株中检出11种耐药基因，其中以四环素类耐药基因tet(A)检出率最高，为95.40%(83/87)；共检测出18种毒力基因，其中侵袭素类mat为优势毒力基因，检出率为85.06%(74/87)；部分耐药基因和耐药表型、耐药基因与毒力基因之间具有相关性。分离株携带12种质粒，主要类型为IncFIB (77.01%，67/87)。接合试验结果显示，共有42株分离菌接合成功，接合转移率为48.28%(42/87)，IncI2、IncHI2、IncX4质粒均成功转移至接合子中，其中IncI2质粒检出率最高(57.14%)。【结论】87株鸡源携带mcr-1基因大肠杆菌多重耐药严重，其耐药基因检出率高，毒力基因种类多样，且部分耐药基因和耐药表型、毒力基因之间存在相关性；48.28%(42/87)菌株的mcr-1基因可以利用IncI2、IncHI2、IncX4质粒作为载体进行传播。研究结果为深入了解福建省鸡源mcr-1基因阳性大肠杆菌的多重耐药情况、毒力特性以及传播机制提供了科学依据。

关键词: 大肠杆菌; 多黏菌素; mcr-1基因; 耐药性; 毒力基因; 接合试验

Abstract: 【Objective】 The purpose of this study was to analyze the correlation between drug resistance and virulence genes of Escherichia coli with polycolistin resistance gene mcr-1 from chickens in Fujian province,and study the plasmid transfer characteristics of mcr-1 resistance gene.【Method】 The sensitivity of 87 strains of chicken Escherichia coli with mcr-1 gene to 11 kinds of antibiotics was detected by microbroth dilution method.PCR was used to detect the drug resistance gene,virulence gene and plasmid type of the isolates,and to analyze the drug resistance phenotype and drug resistance gene of mcr-1 gene positive bacteria,and the correlation between drug resistance gene and virulence gene.Plasmid conjugation test was used to investigate the transfer of mcr-1 gene.【Result】 The results of drug susceptibility test showed that 96.55% (85/87) isolates were multi-drug resistant bacteria,among which the resistance to tetracyclines was the most serious,and the resistance rates of doxycycline and tetracycline were 88.51% (77/87) and 82.76% (72/87),respectively.11 drug resistance genes were detected in the isolates,among which tetracyclines resistance gene tet(A) had the highest detection rate of 95.40% (83/87).A total of 18 virulence genes were detected,among which invasiin-type mat was the dominant virulence gene,with a detection rate of 85.06% (74/87).Some resistance genes and phenotypes,resistance genes and virulence genes were correlated.The isolates carried 12 germplasm,and the main type was IncFIB (77.01%,67/87).The conjugation test results showed that 42 isolates were successfully conjugated,and the conjugation transfer rate was 48.28% (42/87).IncI2,IncHI2 and IncX4 plasmids were successfully transferred to conjugons,and the detection rate of IncI2 plasmid was the highest (57.14%).【Conclusion】 The 87 strains of Escherichia coli with mcr-1 gene from chicken had severe multiple drug resistance,high detection rate of drug resistance genes,various types of virulence genes,and some of the drug resistance genes were correlated with drug resistance phenotype and virulence genes.The mcr-1 gene of 48.28% (42/87) strains could be transmitted by plasmids IncI2,IncHI2 and IncX4.The results provided a scientific basis for further understanding the multi-drug resistance,virulence characteristics and transmission mechanism of mcr-1 gene positive Escherichia coli from chickens in Fujian province.

Key words: Escherichia coli; polycolistin; mcr-1 gene; drug resistance; virulence gene; conjugation test

中图分类号:

S853.74

戴婷婷, 陈海玉, 段楚楚, 刘荣昌, 李宇蓉, 严淑涵, 陈梦诗, 刘露薇, 包银莉, 程艳青, 林炜明, 黄翠琴, 郑新添. 鸡源携带多黏菌素耐药基因mcr-1大肠杆菌耐药性与毒力基因相关性分析[J]. 中国畜牧兽医, 2025, 52(3): 1393-1404.

DAI Tingting, CHEN Haiyu, DUAN Chuchu, LIU Rongchang, LI Yurong, YAN Shuhan, CHEN Mengshi, LIU Luwei, BAO Yinli, CHENG Yanqing, LIN Weiming, HUANG Cuiqin, ZHENG Xintian. Correlation Analysis of Drug Resistance and Virulence Genes of Escherichia coli with Polycolistin Resistance Gene mcr-1 from Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(3): 1393-1404.

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鸡源携带多黏菌素耐药基因mcr-1大肠杆菌耐药性与毒力基因相关性分析

Correlation Analysis of Drug Resistance and Virulence Genes of Escherichia coli with Polycolistin Resistance Gene mcr-1 from Chickens

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