纳米铜对大鼠肝脏毒性的蛋白质组2-DE图谱分析

›› 2012, Vol. 39 ›› Issue (11): 22-26.

纳米铜对大鼠肝脏毒性的蛋白质组2-DE图谱分析

高昭辉^1,2,4, 董书伟^1,4, 薛慧文², 申小云³, 荔霞¹

1. 中国农业科学院兰州畜牧与兽药研究所农业部兽用药物创制重点实验/甘肃省中兽药工程技术中心,甘肃兰州 730050;
2. 甘肃农业大学动物医学院,甘肃兰州 730070;
3. 贵州省毕节学院,贵州毕节 551700;
4. 甘肃省牦牛繁育工程重点实验室,甘肃兰州 730050

收稿日期:2012-05-10 出版日期:2012-11-20 发布日期:2012-11-22
通讯作者: 荔霞(1977-),女,副研究员,博士,主要从事兽药毒理蛋白质组学研究。E-mail: lanzhoulixia@163.com E-mail:lanzhoulixia@163.com
作者简介:高昭辉(1986-),男,山西人,硕士生,主要从事兽医公共卫生学研究。
基金资助:
国家现代农业产业技术体系建设专项资金项目(CARS-40-30)。

2-DE Map Analysis of Proteomics in Rat with the Hepatotoxic Mechanisms of Nanoparticles Copper

GAO Zhao-hui^1,2,4, DONG Shu-wei^1,4, XUE Hui-wen², SHEN Xiao-yun³, LI Xia¹

1. Key Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture, Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS,Lanzhou 730050,China;
2. College of Veterinary Medicine,Gansu Agricultural University, Lanzhou 730070,China;
3. Bijie College of Guizhou Province,Bijie 551700,China;
4. Key Laboratory of Yak Breeding Engineering, Lanzhou 730050,China

Received:2012-05-10 Online:2012-11-20 Published:2012-11-22

摘要/Abstract

摘要： 为获取纳米铜对大鼠肝脏毒性的蛋白质组表达图谱,试验通过构建大鼠纳米铜中毒模型,采用双向凝胶电泳技术获取了大鼠纳米铜中毒组和对照组肝脏的蛋白质2-DE图谱,结合PDQuest 8.0软件分析发现200 mg/kg纳米铜组大鼠肝脏2-DE图谱中有27个蛋白质斑点表达上调,16个蛋白质斑点表达下调,共有43个差异蛋白质点。试验利用蛋白质组学技术研究大鼠纳米铜肝脏蛋白质组学,为在蛋白质水平阐明纳米铜的毒性作用机制奠定基础。

关键词: 蛋白质组学; 2-DE; 纳米铜; 大鼠

Abstract: In order to obtain protein expression map of nanoparticles copper(nano-copper) toxicity in rat liver, this study constructed the model of the nano-copper in rats, and obtained 2-DE maps of nano-copper toxicity in rats group and control group used by two-dimensional gel electrophoresis technique, PDQuest 8.0 analysis showed that the total 43 protein spots significantly differently expressed in rat liver of 200 mg/kg copper nanoparticles group which 27 spots were up and 16 spots were down. The toxicity of nano-copper in rats liver was studied by proteomics approach in the paper, the results would establish the foundation for clarifying the mechanism of the toxic effects of nano-copper at protein level.

Key words: proteomics; 2-DE; nanoparticles copper; rat

中图分类号:

高昭辉, 董书伟, 薛慧文, 申小云, 荔霞. 纳米铜对大鼠肝脏毒性的蛋白质组2-DE图谱分析[J]. , 2012, 39(11): 22-26.

GAO Zhao-hui, DONG Shu-wei, XUE Hui-wen, SHEN Xiao-yun, LI Xia. 2-DE Map Analysis of Proteomics in Rat with the Hepatotoxic Mechanisms of Nanoparticles Copper[J]. , 2012, 39(11): 22-26.

参考文献

1 王心如.毒理学基础[M].第4版.北京:人民卫生出版社,2003.
2 李鸿岩,赵兴绪,张勇,等. 绵羊精子冻融前后差异蛋白质组学研究[J].中国农业科学,2011,44(24):5092~5099.
3 李鸿岩,赵兴绪,张勇,等. 绵羊精浆蛋白质组2-DE图谱的构建及初步分析[J].中国畜牧兽医, 2011, 38(4): 127~131.
4 熊华伟,简少卿,贾小芳,等.富硒酵母对斑点叉尾鮰肝脏的毒性作用及蛋白质组学的影响[J].水产学报,2011,35(7):1008~1014.
5 Cao Z, Han Z, Shao Y, et al. Proteomic analysis of chicken embryonic trachea and kidney tissues after infection in ovo by avian infectious bronchitis coronavirus [J]. Proteome Science, 2011, 9(1):11~27.
6 Chen Z, Meng H, Xing G, et al. Acute toxicological effects of copper nanoparticles in vivo [J]. Toxicol Lett, 2006, 163(2):109~120.
7 Chevallet M, Luche S, Rabilloud T. Silver staining of proteins in polyacrylamide gels[J].Nat Protocol,2006,1(4):1852~1858.
8 Friedm B, Hoving S, Westermeier R. Isoelectric focusing and two-dimensional gel electrophoresis[J]. Method Enzymology, 2009, 463(7): 515~540.
9 Griffit R J, Weil R, Hyndman K A, et al. Exposure to copper nanoparticles causes gill injury and acute lethality in Zebra fish (Danio rerio)[J]. Environ Sci Technol, 2007, 41(23):8178~8186.
10 Kumagat K, Ando Y, Kiyosawa N, et al. Toxic proteomic investigation of the molecular mechanisms of cycloheximide induced hepatocellular apoptos is in rat liver[J].Toxicology,2006,228(2~3):299~309.
11 Lei R H, Wu C Q, Yang B H, et al.Integrated metabolomic analysis of the nano-sized copper particle-induced hepatotoxicity and nephrotoxicity in rats: A rapid in vivo screening method for nanotoxicity [J]. Toxicol Appl Pharmacol, 2008, 232(2):292~301.
12 Meneses L G, Guest P C, Lowrence J, et al. A proteomic investigation of drug induced steatosis in rat liver [J]. Chem Res Toxicol, 2004, 17(5): 605~612.
13 Menese S, Lorente G, Watt A, et al. Identification of early proteomic markers for hepatic steatosis [J]. Chem Res Toxicol, 2006, 19(8): 986~998.
14 Meng H, Chen Z, Xing G M, et al. Ultrahigh reactivity provokes nanotoxicity: Explanation of oral toxicity of nano-copper particles [J]. Toxicol Lett, 2007, 175(1~3):102~110.
15 Nel A, Xia T, Mädler L, et al. Toxic potential of materials at the nanolevel[J].Science, 2006,311 (3): 622~627.
16 Prabhu B M, Ali S F, Murdock R C, et al. Copper nanoparticles exert size and concentration dependent toxicity on somatosensory neurons of rat [J].Nanotoxicology, 2010, 4(2):150~160.
17 Zgoda V, Tikhonova O, Viglinskaya A, et al. Proteomic profiles of induced hepatotoxicity at the sub cellular level [J]. Proteomics, 2006, 6(16): 4662~4670.

[1]	吴涛, 齐云霞, 刘伟, 朱洪龙, 黄冬维, 丁海生, 赵辉玲, 程广龙, 赵小伟. 基于蛋白质组学技术分析初乳和常乳摄入对初生犊牛肝脏蛋白表达的影响[J]. 中国畜牧兽医, 2023, 50(1): 37-45.
[2]	何炎峻, 金圣子, 王爽, 柳可欣, 刘云. 薯蓣皂苷元对顺铂诱导大鼠肾损伤的缓解作用[J]. 中国畜牧兽医, 2022, 49(9): 3633-3642.
[3]	汪瑶, 燕鸽, 刘智慧, 王玉, 辛全超, 曹新悦, 于文会, 姜晓文. 马兜铃酸Ⅰ对大鼠五脏的毒性及功能影响[J]. 中国畜牧兽医, 2022, 49(7): 2757-2767.
[4]	胡文, 霍孔林, 宋星星, 张鑫, 张多妮, 罗荣荣, 徐文镐, 李珣. 促性腺激素抑制激素对SD大鼠性腺生殖功能与糖代谢的影响[J]. 中国畜牧兽医, 2022, 49(6): 2239-2249.
[5]	王璐, 胡盼盼, 程佳, 孙盼盼, 孙娜, 范阔海, 尹伟, 李宏全, 孙耀贵. 穿王消炎粉对LPS诱导大鼠急性肺损伤的治疗作用[J]. 中国畜牧兽医, 2022, 49(4): 1568-1576.
[6]	王一冰, 邝智祥, 叶金玲, 范秋丽, 张盛, 蒋守群. 纳米铜、灵芝孢子粉与大豆异黄酮对1~30日龄清远麻鸡生长、免疫及抗氧化性能的影响[J]. 中国畜牧兽医, 2021, 48(7): 2415-2423.
[7]	代国栋, 闫鸿斌, 李立, 付宝权, 贾万忠. 人兽共患寄生虫病候选疫苗分子筛选方法研究进展[J]. 中国畜牧兽医, 2021, 48(6): 2177-2187.
[8]	孙长超, 高响, 李锋, 梁童, 宁航逸, 王钟煜, 单安山, 石宝明. 短期灌喂民猪源贝莱斯芽孢杆菌M2对SD大鼠生长、免疫及肠道菌群的影响[J]. 中国畜牧兽医, 2021, 48(4): 1264-1274.
[9]	翁秀秀, 刘欣欣, 乐祥鹏, 李万宏, 李发弟. 基于双向电泳技术研究湖羊初乳和常乳乳蛋白质组差异[J]. 中国畜牧兽医, 2021, 48(3): 865-872.
[10]	史晓宇, 宋越, 冯敏, 高珍珍, 杨英. 穴位埋线对运动性疲劳大鼠能量代谢水平的影响[J]. 中国畜牧兽医, 2021, 48(2): 747-754.
[11]	王昱, 秦序, 何九军. 白肉灵芝水提物对脑缺血大鼠海马神经元的保护作用[J]. 中国畜牧兽医, 2021, 48(10): 3864-3871.
[12]	赵军杰, 陈可心, 栾业辉, 程林丽. 恩诺沙星与磺胺二甲嘧啶联合毒性探究[J]. 中国畜牧兽医, 2021, 48(10): 3872-3879.
[13]	和东迁, 陶金忠, 陈丽尧, 王俊奎. 绵羊毛囊及其角蛋白研究进展[J]. 中国畜牧兽医, 2021, 48(1): 248-256.
[14]	韦芊含, 雷伟伟, 陈昕, 赵伟亚, 张蒙, 肖金吒, 张雨梅. 饲用黄丝藻粉的急性毒性和亚慢性毒性试验[J]. 中国畜牧兽医, 2020, 47(5): 1602-1610.
[15]	张多妮, 霍孔林, 李长乐, 张鑫, 宋星星, 胡文, 罗荣荣, 徐文镐, 陈磊, 陈欣仪, 李珣, 王晓晔. RF酰胺相关肽3对大鼠脾脏形态结构及免疫功能的影响[J]. 中国畜牧兽医, 2020, 47(4): 1225-1232.