三氯卡班对大鼠免疫毒性的初步研究

doi:10.16431/j.cnki.1671-7236.2024.10.043

摘要/Abstract

摘要： 【目的】研究三氯卡班(triclocarban，TCC)对大鼠免疫功能及脾脏Toll样受体4(TLR4)/髓样分化因子88(MyD88)/核因子κB(NF-κB)信号通路相关因子表达的影响。【方法】试验选取96只5周龄雄性SD大鼠随机分为4组，分别为玉米油对照组(C组)以及0.1、20、100 mg/kg TCC组(TL、TM和TH组)，每组24只。将不同剂量TCC与玉米油充分混匀，每日灌胃给药1次，C组灌胃等量不含TCC的玉米油，连续60 d。试验结束后，采集大鼠血液和脾脏组织，并检测大鼠血清生化指标、脾淋巴细胞增殖能力、巨噬细胞吞噬指数、血清溶血素水平以及脾脏自然杀伤细胞(natural killer cell，NK)活性。同时通过ELISA法检测大鼠血清免疫球蛋白含量；利用流式细胞术检测大鼠外周血淋巴细胞亚群水平；采用实时荧光定量PCR和Western blotting分别检测TLR4/MyD88/NF-κB通路标志基因以及下游因子的mRNA和蛋白表达水平。【结果】与C组相比，TH组大鼠血清中葡萄糖(Glu)水平显著降低(P<0.05),TM组大鼠血清肌酐(CREA)水平显著升高(P＜0.05)；TM组大鼠脾淋巴细胞增殖能力和血清溶血素水平均显著降低(P＜0.05)。ELISA检测结果显示，TH组大鼠血清中免疫球蛋白A(IgA)、IgM、IgG水平及TM组大鼠血清中IgG水平与C组相比均极显著降低(P＜0.01)。流式细胞检测结果显示，不同剂量TCC组大鼠外周血CD3⁺、CD4⁺ T细胞占比和CD4⁺/CD8⁺比值与C组相比均无显著差异(P＞0.05)。实时荧光定量PCR结果显示，与C组相比，不同剂量TCC组大鼠脾脏中TLR4、MyD88、IL-1β的mRNA相对表达量均显著或极显著升高(P＜0.05；P＜0.01)。Western blotting 结果显示，与C组相比，TM组大鼠脾脏中TLR4、MyD88和NF-κB蛋白表达量均极显著升高(P＜0.01)。【结论】 TCC对大鼠的细胞免疫和体液免疫能够产生一定的毒性作用，能显著激活大鼠脾脏TLR4/MyD88/NF-κB信号通路，且可能通过靶向该信号通路来损伤大鼠的免疫系统，诱发大鼠的免疫毒性。

关键词: 三氯卡班; 大鼠; Toll样受体4(TLR4); 髓样分化因子88(MyD88); 核因子κB(NF-κB); 免疫毒性

Abstract: 【Objective】 The purpose of this study was to investigate the effects of triclocarban (TCC) on immune function and the expression of Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (MyD88)/nuclear factor κB (NF-κB) signaling pathway in spleen of rats.【Method】 A total of 96 5-week-old male SD rats were randomly divided into 4 groups： Corn oil control group (group C) and 0.1，20 and 100 mg/kg TCC groups (TL，TM and TH)，with 24 rats in each group. Different doses of TCC were thoroughly mixed with corn oil and given intragastric administration once a day. Rats in group C were given intragastric administration of the same amount of corn oil without TCC，the experiment lasted for 60 days. After the experiment，blood and spleen tissues of rats were collected，and serum biochemical indexes，splenic lymphocyte proliferation capacity，macrophage phagocytosis index，serum hemolysin level and spleen natural killer cell (NK) activity were detected. The serum immunoglobulin content of rats was detected by ELISA. The lymphocyte subsets in peripheral blood of rats were detected by flow cytometry. Real-time quantitative PCR and Western blotting were used to detect mRNA and protein expression levels of TLR4/MyD88/NF-κB pathway marker genes and downstream factors，respectively.【Result】 Compared with group C，serum glucose (Glu) level in TH group was significantly decreased (P＜0.05), and serum creatinine (CREA) level in TM group was significantly increased (P＜0.05). The splenic lymphocyte proliferation ability and serum hemolysin level of TM group were significantly decreased (P＜0.05). ELISA results showed that the levels of immunoglobulin A (IgA)，IgM and IgG in serum of rats in TH group and IgG in serum in TM group were extremely significantly decreased compared with group C (P＜0.01). Flow cytometry results showed that，compared with group C ，there were no significant differences in the proportion of CD3⁺，CD4⁺ T cells and the ratio of CD4⁺ and CD8⁺ in peripheral blood of rats in different doses of TCC groups (P ＞ 0.05). Real-time quantitative PCR results showed that，compared with group C，the mRNA relative expression levels of TLR4，MyD88 and IL-1β in spleen of rats in different doses of TCC groups were significantly or extremely significantly increased (P ＜ 0.05 or P ＜ 0.01). Western blotting results showed that compared with group C，the expressions of TLR4，MyD88 and NF-κB proteins in spleen of TM group were extremely significantly increased (P ＜ 0.01).【Conclusion】 TCC could produce certain toxic effects on cellular and humoral immunity of rats，and significantly activate the TLR4/MyD88/NF-κB signaling pathway in rat spleen. TCC might damage the immune system of rats by targeting the TLR4/MyD88/NF-κB pathway in the spleen and induce immunotoxicity in rats.

Key words: triclocarban; rat; Toll-like receptor 4 (TLR4); myeloid differentiation factor 88(MyD88); nuclear factor κB (NF-κB); immunotoxicity

中图分类号:

S854.4⁺3

引用本文

李芳芳, 隋鑫, 闵婷玉, 黄涛, 徐在品. 三氯卡班对大鼠免疫毒性的初步研究[J]. 中国畜牧兽医, 2024, 51(10): 4626-4634.

LI Fangfang, SUI Xin, MIN Tingyu, HUANG Tao, XU Zaipin. Preliminary Study of Triclocarban on Immunotoxicity in Rats[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(10): 4626-4634.

使用本文

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chvm.net/CN/10.16431/j.cnki.1671-7236.2024.10.043

https://www.chvm.net/CN/Y2024/V51/I10/4626

参考文献

[1] HALDEN R U，PAULL D H. Co-occurrence of triclocarban and triclosan in U.S. water resources[J]. Environmental Science & Technology，2005,39(6):1420-1426.
[2] 杨基峰，郑丽英，靳俊玲，等. 水体中羟基自由基介导的三氯卡班氧化机制的量子化学研究[J]. 环境科学学报，2022,42(11):77-84.YANG J F，ZHENG L Y，JIN J L，et al. Oxidation mechanism of triclocarban in aqueous phase mediated by hydroxyl radicals based on guantum chemical study[J]. Acta Scientiae Circumstantiae，2022,42(11):77-84. (in Chinese)
[3] 张鹏，张维，齐丽娟，等. 三氯生的急性毒性、局部毒性及亚慢性经皮毒性试验研究[J]. 毒理学杂志，2014,28(5):413-415.ZHANG P，ZHANG W，QI L J，et al. Experimental study on acute toxicity，local toxicity and subchronic transdermal toxicity of triclosan[J]. Journal of Toxicology，2014,28(5):413-415.(in Chinese)
[4] ZHAO J L，ZHANG Q Q，CHEN F，et al. Evaluation of triclosan and triclocarban at river basin scale using monitoring and modeling tools: Implications for controlling of urban domestic sewage discharge[J]. Water Research，2013,47(1):395-405.
[5] IACOPETTA D，CATALANO A，CERAMELLA J，et al. The different facets of triclocarban: A review[J]. Molecules，2021,26(9):2811.
[6] HALDEN R U，PAULL D H. Analysis of triclocarban in aquatic samples by liquid chromatography electrospray ionization mass spectrometry[J]. Environmental Science & Technology，2004,38(18):4849-4855.
[7] LI W，ZHANG W，CHANG M，et al. Metabonomics reveals that triclocarban affects liver metabolism by affecting glucose metabolism，beta-oxidation of fatty acids，and the TCA cycle in male mice[J]. Toxicology Letters，2018,299:76-85.
[8] LI X，WANG W，WANG X，et al. Differential immunotoxicity effects of triclosan and triclocarban on larval zebrafish based on RNA-Seq and bioinformatics analysis[J]. Aquatic Toxicology (Amsterdam，Netherlands)，2023,262:106665.
[9] XU J，QIAN Q，XIA M，et al. Trichlorocarban induces developmental and immune toxicity to zebrafish (Danio rerio) by targeting TLR4/MyD88/NF-kappaB signaling pathway[J]. Environmental Pollution, 2021,273:116479.
[10] MIURA Y，CHEN X，YAMADA S，et al. Triclocarban-induced change in intracellular Ca²⁺ level in rat thymocytes: Cytometric analysis with Fluo-3 under Zn²⁺-free conditions[J]. Environmental Toxicology and Pharmacology，2014,37(2):563-570.
[11] KANBARA Y，MURAKANE K，NISHIMURA Y，et al. Nanomolar concentration of triclocarban increases the vulnerability of rat thymocytes to oxidative stress[J]. The Journal of Toxicological Sciences，2013,38(1):49-55.
[12] DONG M，XU X，HUANG Q，et al. Dose-dependent effects of triclocarban exposure on lipid homeostasis in rats[J]. Chemical Research in Toxicology，2019,32(11):2320-2328.
[13] TIAN L，YAN J，LI K，et al. Ozone exposure promotes pyroptosis in rat lungs via the TLR2/4-NF-kappaB-NLRP3 signaling pathway[J]. Toxicology，2021,450:152668.
[14] WANG K，ZHANG X，LI X，et al. Non-weight-bearing exercise attenuates papain-induced knee osteoarthritis in rats via the TLR4/MyD88/NF-kappaB signaling pathway[J]. Journal of Orthopaedic Surgery and Research，2023,18(1):695.
[15] LIN S J，CAO L X，CHENG S B，et al. Effect of acupuncture on the TLR2/4-NF-kappaB signalling pathway in a rat model of traumatic brain injury[J]. Acupuncture in Medicine，2018,36(4):247-253.
[16] 苏崇莹，牛嘉璐，古浚杰，等. PA28γ在免疫相关疾病中的作用及机制研究进展[J].中国免疫学杂志，2024,40(1):192-201.SU C Y，NIU J L，GU J J，et al. Research progress on role and mechanism of PA28γ in immune-related diseases[J]. Chinese Journal of Immunology，2024,40(1):192-201.(in Chinese)
[17] LUSTER M I，PAIT D G，PORTIER C，et al. Qualitative and quantitative experimental models to aid in risk assessment for immunotoxicology[J]. Toxicology Letters，1992,64-65:71-78.
[18] CHRZ J，DVORAKOVA M，KEJLOVA K，et al. The potential for genotoxicity，mutagenicity and endocrine disruption in triclosan and triclocarban assessed through a combination of in vitro methods[J]. Journal of Xenobiotics，2023,14(1):15-30.
[19] ANDERSON S E，MEADE B J，LONG C M，et al. Investigations of immunotoxicity and allergic potential induced by topical application of triclosan in mice[J]. Journal of Immunotoxicology，2016,13(2):165-172.
[20] 陈昕，韩玲玲，韦芊含，等. 茶树油提取物粉对大鼠免疫功能的影响[J].中国兽医杂志，2020,56(9):74-77.CHEN X，HAN L L，WEI Q H，et al. Effect of the powder of tea tree oil extract on immune function in rats[J].Chinese Journal of Veterinary Medicine，2020,56(9):74-77.(in Chinese)
[21] 张龙，杨明亮，刘燕群，等. 三氯生对昆明小鼠胸腺及脾脏等免疫器官的影响[J].公共卫生与预防医学，2021,32(5):48-51.ZHANG L，YANG M L，LIU Y Q，et al. The effect of triclosan on the immune function of Kunming mice[J]. Journal of Public Health and Preventive Medicine，2021,32(5):48-51.(in Chinese)
[22] WANG H，LI X，WANG W，et al. Immunotoxicity induced by triclocarban exposure in zebrafish triggering the risk of pancreatic cancer[J]. Environmental Pollution，2023,325:121458.
[23] 王军亮，牛玉祥，陆新章，等. 姜黄素对疫病流行区肥育猪血清SOD和IgG表达的影响[J]. 动物医学进展，2023,44(4):69-71.WANG J L，NIU Y X，LU X Z，et al. Effects of curcumin on the expression of serum SOD and IgG in finishing pigs of epidemic area[J]. Progress in Veterinary Medicine，2023,44(4):69-71.(in Chinese)
[24] 鲍俊达，王平，王文佳，等. 基于TLR4/MyD88/NF-κB信号通路探讨复方石斛汤对气道炎症模型大鼠的影响[J]. 中医学报，2023,38(8):1583-1592.BAO J D，WANG P，WANG W J，et al. Effect of compound Shihu decoction on airway inflammation model rats based on TLR4/MyD88/NF-κB signal pathway[J].Acta Chinese Medicine，2023,38(8):1583-1592.(in Chinese)
[25] 付智慧，周霖，聂安政. 基于TLR4/MyD88/NF-κB与p38 MAPK信号通路研究麻杏石甘汤减轻咳嗽变异性哮喘大鼠炎症反应的作用及机制[J]. 中国中药杂志，2024,49(4):1000-1006.FU Z H，ZHOU L，NIE A Z. Effect and mechanism of Maxing Shigan decoction on reducing inflammatory response in rats with cough variant asthma via TLR4/MyD88/NF-κB and p38 MAPK signaling pathways[J]. China Journal of Chinese Materia Medica，2024,49(4):1000-1006.(in Chinese)

相关文章 15

[1] 孙悦, 田雪, 杨春雪, 徐恩爽, 郑家三. 番茄红素对顺铂所致大鼠脏器损伤的保护效果评价[J]. 中国畜牧兽医, 2024, 51(8): 3247-3255.

[2] 苏圆圆, 于澄元, 张密霞, 张艳军, 庄朋伟. 健脾祛湿方治疗非酒精性脂肪肝大鼠的作用研究[J]. 中国畜牧兽医, 2024, 51(8): 3662-3675.

[3] 李倩倩, 蔺思函, 王丽媛, 杨兵兵, 张明达, 沈秀丽, 杜志强. 猪种布鲁氏菌TIR结构域蛋白的生物信息学分析、重组表达及蛋白互作研究[J]. 中国畜牧兽医, 2023, 50(8): 3303-3312.

[4] 汪星萍, 曹家慧, 苟丹丹, 邵义文, 迟士鹏, 杨春雪, 徐恩爽. 表没食子儿茶素-3-没食子酸酯缓解顺铂所致大鼠急性肾损伤效果评价[J]. 中国畜牧兽医, 2023, 50(12): 5186-5193.

[5] 何炎峻, 金圣子, 王爽, 柳可欣, 刘云. 薯蓣皂苷元对顺铂诱导大鼠肾损伤的缓解作用[J]. 中国畜牧兽医, 2022, 49(9): 3633-3642.

[6] 汪瑶, 燕鸽, 刘智慧, 王玉, 辛全超, 曹新悦, 于文会, 姜晓文. 马兜铃酸Ⅰ对大鼠五脏的毒性及功能影响[J]. 中国畜牧兽医, 2022, 49(7): 2757-2767.

[7] 胡文, 霍孔林, 宋星星, 张鑫, 张多妮, 罗荣荣, 徐文镐, 李珣. 促性腺激素抑制激素对SD大鼠性腺生殖功能与糖代谢的影响[J]. 中国畜牧兽医, 2022, 49(6): 2239-2249.

[8] 王璐, 胡盼盼, 程佳, 孙盼盼, 孙娜, 范阔海, 尹伟, 李宏全, 孙耀贵. 穿王消炎粉对LPS诱导大鼠急性肺损伤的治疗作用[J]. 中国畜牧兽医, 2022, 49(4): 1568-1576.

[9] 乔春雨, 刘家和, 张博熙, 何雨曦, 郑雨微, 吕红明. 黄曲霉毒素B₁致病机制及防治研究进展[J]. 中国畜牧兽医, 2022, 49(2): 765-775.

[10] 孙长超, 高响, 李锋, 梁童, 宁航逸, 王钟煜, 单安山, 石宝明. 短期灌喂民猪源贝莱斯芽孢杆菌M2对SD大鼠生长、免疫及肠道菌群的影响[J]. 中国畜牧兽医, 2021, 48(4): 1264-1274.

[11] 史晓宇, 宋越, 冯敏, 高珍珍, 杨英. 穴位埋线对运动性疲劳大鼠能量代谢水平的影响[J]. 中国畜牧兽医, 2021, 48(2): 747-754.

[12] 王昱, 秦序, 何九军. 白肉灵芝水提物对脑缺血大鼠海马神经元的保护作用[J]. 中国畜牧兽医, 2021, 48(10): 3864-3871.

[13] 赵军杰, 陈可心, 栾业辉, 程林丽. 恩诺沙星与磺胺二甲嘧啶联合毒性探究[J]. 中国畜牧兽医, 2021, 48(10): 3872-3879.

[14] 韦芊含, 雷伟伟, 陈昕, 赵伟亚, 张蒙, 肖金吒, 张雨梅. 饲用黄丝藻粉的急性毒性和亚慢性毒性试验[J]. 中国畜牧兽医, 2020, 47(5): 1602-1610.

[15] 张多妮, 霍孔林, 李长乐, 张鑫, 宋星星, 胡文, 罗荣荣, 徐文镐, 陈磊, 陈欣仪, 李珣, 王晓晔. RF酰胺相关肽3对大鼠脾脏形态结构及免疫功能的影响[J]. 中国畜牧兽医, 2020, 47(4): 1225-1232.

编辑推荐

Metrics

阅读次数

全文

摘要

本文评价

摘要

参考文献

相关文章

编辑推荐

Metrics

本文评价

回顶部

三氯卡班对大鼠免疫毒性的初步研究

Preliminary Study of Triclocarban on Immunotoxicity in Rats

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	孙悦, 田雪, 杨春雪, 徐恩爽, 郑家三. 番茄红素对顺铂所致大鼠脏器损伤的保护效果评价[J]. 中国畜牧兽医, 2024, 51(8): 3247-3255.
[2]	苏圆圆, 于澄元, 张密霞, 张艳军, 庄朋伟. 健脾祛湿方治疗非酒精性脂肪肝大鼠的作用研究[J]. 中国畜牧兽医, 2024, 51(8): 3662-3675.
[3]	李倩倩, 蔺思函, 王丽媛, 杨兵兵, 张明达, 沈秀丽, 杜志强. 猪种布鲁氏菌TIR结构域蛋白的生物信息学分析、重组表达及蛋白互作研究[J]. 中国畜牧兽医, 2023, 50(8): 3303-3312.
[4]	汪星萍, 曹家慧, 苟丹丹, 邵义文, 迟士鹏, 杨春雪, 徐恩爽. 表没食子儿茶素-3-没食子酸酯缓解顺铂所致大鼠急性肾损伤效果评价[J]. 中国畜牧兽医, 2023, 50(12): 5186-5193.
[5]	何炎峻, 金圣子, 王爽, 柳可欣, 刘云. 薯蓣皂苷元对顺铂诱导大鼠肾损伤的缓解作用[J]. 中国畜牧兽医, 2022, 49(9): 3633-3642.
[6]	汪瑶, 燕鸽, 刘智慧, 王玉, 辛全超, 曹新悦, 于文会, 姜晓文. 马兜铃酸Ⅰ对大鼠五脏的毒性及功能影响[J]. 中国畜牧兽医, 2022, 49(7): 2757-2767.
[7]	胡文, 霍孔林, 宋星星, 张鑫, 张多妮, 罗荣荣, 徐文镐, 李珣. 促性腺激素抑制激素对SD大鼠性腺生殖功能与糖代谢的影响[J]. 中国畜牧兽医, 2022, 49(6): 2239-2249.
[8]	王璐, 胡盼盼, 程佳, 孙盼盼, 孙娜, 范阔海, 尹伟, 李宏全, 孙耀贵. 穿王消炎粉对LPS诱导大鼠急性肺损伤的治疗作用[J]. 中国畜牧兽医, 2022, 49(4): 1568-1576.
[9]	乔春雨, 刘家和, 张博熙, 何雨曦, 郑雨微, 吕红明. 黄曲霉毒素B₁致病机制及防治研究进展[J]. 中国畜牧兽医, 2022, 49(2): 765-775.
[10]	孙长超, 高响, 李锋, 梁童, 宁航逸, 王钟煜, 单安山, 石宝明. 短期灌喂民猪源贝莱斯芽孢杆菌M2对SD大鼠生长、免疫及肠道菌群的影响[J]. 中国畜牧兽医, 2021, 48(4): 1264-1274.
[11]	史晓宇, 宋越, 冯敏, 高珍珍, 杨英. 穴位埋线对运动性疲劳大鼠能量代谢水平的影响[J]. 中国畜牧兽医, 2021, 48(2): 747-754.
[12]	王昱, 秦序, 何九军. 白肉灵芝水提物对脑缺血大鼠海马神经元的保护作用[J]. 中国畜牧兽医, 2021, 48(10): 3864-3871.
[13]	赵军杰, 陈可心, 栾业辉, 程林丽. 恩诺沙星与磺胺二甲嘧啶联合毒性探究[J]. 中国畜牧兽医, 2021, 48(10): 3872-3879.
[14]	韦芊含, 雷伟伟, 陈昕, 赵伟亚, 张蒙, 肖金吒, 张雨梅. 饲用黄丝藻粉的急性毒性和亚慢性毒性试验[J]. 中国畜牧兽医, 2020, 47(5): 1602-1610.
[15]	张多妮, 霍孔林, 李长乐, 张鑫, 宋星星, 胡文, 罗荣荣, 徐文镐, 陈磊, 陈欣仪, 李珣, 王晓晔. RF酰胺相关肽3对大鼠脾脏形态结构及免疫功能的影响[J]. 中国畜牧兽医, 2020, 47(4): 1225-1232.