番茄红素对顺铂所致大鼠脏器损伤的保护效果评价

doi:10.16431/j.cnki.1671-7236.2024.08.003

摘要/Abstract

摘要： 【目的】评价不同浓度番茄红素(lycopene,LCP)对顺铂(CDDP)诱导大鼠脏器损伤的保护效果。【方法】将30只雄性Wistar大鼠随机分为5组(n＝6),分别为对照组(CON)、CDDP组及不同浓度LCP处理组(5 mg/kg,LLCP组;10 mg/kg,MLCP组;50 mg/kg,HLCP组)。CON和CDDP组大鼠每天灌胃1 mL/kg玉米油,LLCP、MLCP和HLCP组大鼠每天灌胃相应浓度的LCP,连续灌胃28 d。除CON组外,其余各组在第26天单次腹腔注射CDDP(7 mg/kg)。试验结束后采集大鼠胃、小肠(十二指肠、空肠和回肠)、肝脏、肾脏组织及血液样本。通过生理和生化指标检测、HE染色、ELISA和Western blotting方法评价不同浓度LCP在CDDP所致大鼠急性损伤中的保护作用。【结果】与CON组相比,CDDP组大鼠体重、采食量和饮水量均极显著或显著下降(P＜0.01;P＜0.05),血清中谷草转氨酶(AST)、尿素氮(BUN)、肌酐(CRE)等6项生化指标水平、丙二醛(MDA)、白细胞介素-8(IL-8)、IL-6、肿瘤坏死因子-α(TNF-α)含量均显著或极显著升高(P＜0.05;P＜0.01),超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性极显著下降(P＜0.01),大鼠胃、小肠、肝脏和肾脏组织病理学损伤严重。与CDDP组相比,MLCP组大鼠体重、采食量和饮水量均显著升高(P＜0.05),血清中AST、ALT等6项生化指标水平以及MDA、IL-8、TNF-α和IL-6含量均显著降低(P＜0.05),脏器组织病理学损伤减轻。Western blotting结果显示,与CON组相比,CDDP组大鼠不同组织中TNF-α和IL-6蛋白表达量均极显著升高(P＜0.01),IL-10蛋白表达量极显著下降(P＜0.01)。与CDDP组相比,MLCP组大鼠各组织中TNF-α和IL-6蛋白表达量均极显著或显著降低(P＜0.01;P＜0.05),IL-10蛋白表达量均显著或极显著升高(P＜0.01;P＜0.05)。【结论】LCP能通过抑制氧化应激和炎症反应改善CDDP诱导的大鼠胃、小肠、肝脏和肾脏损伤,本试验条件下10 mg/kg LCP作用效果最佳。

关键词: 番茄红素; 顺铂; 大鼠; 脏器损伤; 炎症

Abstract: 【Objective】 The aim of this study was to evaluate the protective effect of lycopene (LCP) on organ injury induced by cisplatin (CDDP) in rats.【Method】 Thirty male Wistar rats were randomly divided into 5 groups (n＝6),which were control group (CON),CDDP and different LCP treatment groups (5 mg/kg,LLCP group;10 mg/kg,MLCP group;50 mg/kg,HLCP group).Rats in CON and CDDP groups were given 1 mL/kg corn oil per day,and rats in LLCP,MLCP and HLCP groups were given the corresponding concentration of LCP per day,and consecutive for 28 days.Except CON group,the other groups were given a single intraperitoneal injection of CDDP (7 mg/kg) on day 26.After the experiment,samples of stomach,small intestine (duodenum,jejunum and ileum),liver,kidney and blood were collected.The protective effects of different concentrations of LCP on CDDP-induced acute injury in rats were evaluated by physiological and biochemical indexes detection,HE staining,ELISA and Western blotting.【Result】 Compared with CON group,body weight,feed intake and water intake of rats in CDDP group were extremely significantly or significantly decreased (P＜0.01 or P＜0.05).Serum levels of aspartate aminotransferase (AST),urea nitrogen (BUN),creatinine (CRE) and malondialdehyde (MDA),interleukin-8 (IL-8),IL-6 and tumor necrosis factor-α (TNF-α) were significantly or extremely significantly increased (P＜0.05 or P＜0.01).The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were extremely significantly decreased (P＜0.01),and the histopathological injuries of stomach,small intestine,liver and kidney were serious.Compared with CDDP group,the body weight,feed intake and water intake of rats in MLCP group were significantly increased (P＜0.05),the levels of 6 biochemical indexes such as AST and ALT and the contents of MDA,IL-8,TNF-α and IL-6 in serum were significantly decreased (P＜0.05),and the organ histomathological damage was alleviated.Western blotting results showed that compared with CON group,the expression of TNF-α and IL-6 proteins in different tissues of rats in CDDP group were extremely significantly increased (P＜0.01),while the expression of IL-10 protein was extremely significantly decreased (P＜0.01).Compared with CDDP group,the expression of TNF-α and IL-6 proteins in all tissues of MLCP group were extremely significantly or significantly decreased (P＜0.01 or P＜0.05),the expression of IL-10 protein was extremely significantly or significantly increased (P＜0.01 or P＜0.05).【Conclusion】 LCP could improve the stomach,small intestine,liver and kidney injury in CDDP-induced rats by inhibiting oxidative stress and inflammation.Under this experiment conditions,10 mg/kg LCP had the best effect.

Key words: lycopene; cisplatin; rat; organ injury; inflammation

中图分类号:

S853

孙悦, 田雪, 杨春雪, 徐恩爽, 郑家三. 番茄红素对顺铂所致大鼠脏器损伤的保护效果评价[J]. 中国畜牧兽医, 2024, 51(8): 3247-3255.

SUN Yue, TIAN Xue, YANG Chunxue, XU Enshuang, ZHENG Jiasan. Evaluation of the Protective Effect of Lycopene on Organ Injury Induced by Cisplatin in Rats[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(8): 3247-3255.

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