关键词: 牡荆水提物; 腹泻; 炎症因子; 通道蛋白; 急性期蛋白

Abstract: 【Objective】 This study was aimed to explore the therapeutic effect of aqueous extract of Vitex negundo L.var.cannabifolia (Sieb.et Zucc.) Hand.-Mazz.on diarrhea induced by castor oil in mice, evaluate its anti diarrhea effect, and provide theoretical basis for the development of new natural drugs or plant extract feed additives.【Method】 48 SPF KM mice weighing 18-22 g were selected.The mice were randomly divided into six groups which including control, model, loperamide, Vitex negundo L.var.cannabifolia(Sieb.et Zucc.)Hand.- Mazz.aqueous extract high, medium and low dose groups, 8 in each group, half male and half female.Mice were gavaged with 16, 8 and 4 g/kg BW in high, medium and low dose groups, respectively, equal doses of saline were gavaged in control and model groups, and 5 mg/kg BW loperamide were practiced in loperamide group.The mice were gavaged for 5 d.0.5 h after administration on the 5th day, mice in model, loperamide, and the high, medium and low dose groups were gavaged with 0.5 mL castor oil for modeling, and mice in control group were gavaged with the same amount of saline.The mice were caged one by one after modeling and were observed the diarrhea for 4 h.After 4 h, blood samples were collected and mice were executed by cervical dislocation, two copies of liver and small intestine were randomly taken from 4 mice in each group, and one copy was for tissue section, the other copy was for RNA extraction to detect mRNA expression of jejunal channel protein and acute phase protein of liver.【Result】 The diarrhea score and diarrhea index of mice in model group were extremely significantly higher than those in control group (P＜0.01), indicating that the castor oil diarrhea model was manufactured successfully.The diarrhea score and diarrhea index of mice in loperamide and high dose groups were extremely significantly or significantly lower than those in model group (P＜0.01 or P＜0.05).The concentration of interleukin-1β (IL-1β), IL-6 and IL-10 in serum of mice in high dose group were significantly or extremely significantly lower than those in model group (P＜0.05 or P＜0.01).The histopathological results of liver showed that the hepatic cords were clear and the hepatocytes were closely arranged in high, medium and low dose groups, and only a few hepatocytes were mildly swollen and infiltrated with a few inflammatory cells.The results of the intestine histopathology showed that the mucosal layer, muscle layer and plasma layer of jejunum were clearly visible in high, medium and low dose groups, and a large number of vacuoles were seen in villous epithelium of mucosal layer, but no obvious necrosis or inflammatory reactions were observed.Compared with model group, the expression of NHE8, NHE3, AQP3 and AQP4 of jejunum in mice were not significantly different in high dose group (P＞0.05), while the expression of NHE2 was significantly lower (P＜0.05), and the expression of the acute phase proteins TRF and CRP were significantly lower (P＜0.05).【Conclusion】 The therapeutic effect of aqueous extract of Vitex negundo L.var.cannabifolia (Sieb.et Zucc.) Hand.-Mazz.on castor oil-induced diarrhea model in mice was good, and could protect liver and small intestine mucosa.The mechanism might be related to the reduction of IL-6 and IL-10 levels in serum and decreasing NHE2 expression and the inhibition of TRF and CRP expression.

Key words: aqueous extract of Vitex negundo L.var.cannabifolia (Sieb.et Zucc.) Hand.-Mazz.; diarrhea; inflammatory factors; channel proteins; acute phase proteins