关键词: Flt-1基因; 绵羊; 组织表达

Abstract: The total RNA was extracted from the oviduct,endometrium and corpus luteum of the female sheep during gestational period,according to published complementary deoxyribonucleic acid sequence of the fms-like tyrosine kinase-1 Flt-1 gene,we designed and synthesized the primers. The transcription-polymerase chain ruction was used to examined the expression of (Flt-1),the products of the transcription-polymerase chain ruction were ligated with vector T and the sequencing were analysed,a β-Actin gene was amplified as the reference gene. The gene was used for interion contrast and the amplification of Flt-1 were examined with the agarose gel electrophoresis,using Kodak Science 1 D,Bandscan image analysis software,we received the expressive amount of Flt-1 gene in the different gential tract tissue. The results indicated that the 82 bp fragment of Flt-1 gene were received in the different gential tract tissue of the female sheep during gestion.The expressive amount of Flt-1 gene in the different gential tract tissue of the female sheep was different. It was said that Flt-1 gene play important role in the female sheep during gestion.

Key words: fms-like tyrosine kinase-1(Flt-1) gene; sheep; tissue expression