›› 2011, Vol. 38 ›› Issue (4): 137-139.

• 遗传繁育 • 上一篇    下一篇

4个家兔品种MHC-DQA基因外显子2 Mbo Ⅱ酶切多态性分析

邝良德, 谢晓红, 黄邓萍, 易军, 雷岷, 任永军, 郭志强, 李丛艳, 郑洁   

  1. 四川省畜牧科学研究院,四川成都 610066
  • 收稿日期:2010-09-15 修回日期:1900-01-01 出版日期:2011-04-20 发布日期:2011-04-20
  • 通讯作者: 谢晓红

Polymorphism Analysis on Exon 2 of MHC-DQA Gene in 4 Rabbit Breeds

KUANG Liang-de, XIE Xiao-hong, HUANG Deng-ping, YI Jun, LEI Min, REN Yong-jun, GUO Zhi-qiang, LI Cong-yan, ZHENG Jie   

  1. Sichuan Province Institute of Animal Husbandry Research, Chengdu 610066,China
  • Received:2010-09-15 Revised:1900-01-01 Online:2011-04-20 Published:2011-04-20

摘要: 采用PCR-RFLP方法对4个不同品种家兔MHC-DQA基因外显子2的遗传多态性进行检测,并进行聚类分析。结果表明,在家兔MHC-DQA基因外显子2的第103 bp处表现出多态性;χ2适合性检验结果表明,Mbo Ⅱ酶切位点在齐卡大型新西兰白兔和齐卡巨型白兔群体均没有达到Hardy-Weinberg平衡状态(P<0.05),而在齐兴肉兔和加利福尼亚兔群体均达到Hardy-Weinberg平衡状态(P>0.05);聚类分析结果表明,齐卡大型新西兰白兔与齐卡巨型白兔亲缘关系最近。试验结果为进一步利用MHC进行抗病育种提供了分子生物学基础。

关键词: 家兔; MHC-DQA; 遗传多态性

Abstract: Genetic polymorphism on exon 2 of MHC-DQA gene was investigated in 4 rabbits breeds by PCR-RFLP method with Mbo Ⅱ enzyme, and cluster analysis was also performed. The results showed that there was a polymorphism at position 103 bp of exon 2 of MHC-DQA. The χ2 tests showed that Mbo Ⅱ restriction enzyme sites were not in Hardy-Weinberg equilibrium among ZIKA large New Zealand white rabbits and ZIKA giant white rabbits(P<0.05), while in Hardy-Weinberg equilibrium among California rabbit and Qixin rabbit(P>0.05), cluster analysis showed that ZIKA large New Zealand white rabbits had the closest genetic relationship with ZIKA giant white rabbits. The results obtained in this study will provide a molecular biological basis for further analysis of relationship of MHC polymorphism and disease resistance in rabbit.

Key words: rabbit; MHC-DQA; genetic polymorphism

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