›› 2011, Vol. 38 ›› Issue (3): 69-72.

• 生物技术 • 上一篇    下一篇

玉米蛋白多肽对鹅不同组织脂肪酸合成酶定位表达的影响

栾新红1，曹中赞2，曹敏建1，石娇1，吴高峰1，姜丽1   

  1. (1.沈阳农业大学畜牧兽医学院，辽宁沈阳 110866； 2.中国农业科学院哈尔滨兽医研究所，黑龙江哈尔滨 150001)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-03-20 发布日期:2011-03-20
  • 通讯作者: 曹敏建

Effect of Corn Protein Polypeptide on Fatty Acid Synthetase Expressing Location in Different Tissues of Geese

LUAN Xin-hong1，CAO Zhong-zan2，CAO Min-jian1，SHI Jiao1，WU Gao-feng1，JIANG Li1   

  1. （1.College of Animal Science and Veterinary Medicine,Shenyang Agricultural University, Shenyang 110866,China；2.Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences, Harbin 150001,China）
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-03-20 Published:2011-03-20
  • Contact: CAO Min-jian

摘要： 选用免疫组化技术，探讨玉米蛋白多肽对鹅不同组织脂肪酸合成酶（fatty acid synthase，FAS）定位表达的影响。选取1日龄昌图豁鹅240只，公母各半，随机分为4组，每组设6个重复，每个重复10只。对照组饲喂基础日粮低、中、高剂量组分别于基础日粮中添加玉米蛋白多肽100、300、500 mg/kg。于30和60日龄分别采集动物肝脏和皮下脂肪组织，运用免疫组化法分析两种组织中FAS的表达量及分布。结果表明，鹅两种组织中FAS的表达主要分布在肝脏内皮细胞胞质和胞核及脂肪细胞膜上，且随着鹅日龄增加，FAS含量均增加；玉米蛋白多肽的添加可促进鹅肝脏中FAS的表达，抑制脂肪组织中FAS的表达；高、中、低3个剂量组肝脏中FAS含量，鹅60日龄时分别比30日龄升高47.75%、36.21%、158.74%，增幅均高于对照组（11.00%）；而60日龄时，3个剂量组脂肪组织中FAS含量分别比30日龄降低22.34%、46.43%、49.17%。结果提示，玉米蛋白多肽呈现双重作用，在鹅日粮中较长时间低剂量水平（100 mg/kg日粮）添加，可有效促进肝脏中FAS表达，降低脂肪组织中FAS含量，从而有利于调控机体脂类代谢，提高动物胴体品质。

关键词: 玉米蛋白多肽; 免疫组织化学法; 脂肪酸合成酶; 鹅

Abstract: The immunohistochemical techniques were used in this experiment to discuss the effect of the corn protein polypeptide on fat fatty synthase (FAS) expression location in different tissues of geese. 240 one-day-old Changtu huoyan geese were randomly divided into 4 groups, each group set six repeats, each repeat with 10 geese. Among them, one control group was fed basal diet, the other three doses groups, per kilogram of basal diets supplemented with corn protein polypeptide 100, 300 and 500 mg, respectively. Then in 30-day-old and 60-day-old, the liver tissue and subcutaneous adipose of geese were collected to be applied in analyzing the expression and distribution of FAS by immunohistochemical method. The results indicated that in liver tissue and adipose tissue of geese, the FAS expressing was mainly distributed in the liver endothelial cell cytoplasm and nucleus and adipocyte membrane. With the increase of age, the content of FAS in liver and fat tissue of geese increased.Add corn protein polypeptide could promote the FAS expressing in livers of geese and restrain expressing in adipose tissue. Compared 60-day-old with 30-day-old, the FAS expression of livers in high, medium and low dose group, increased 47.75%、36.21% and 158.74%, respectively, but 11.00% in control group. Meantime corn protein polypeptide could restrain the FAS expression of the adipose tissue, decrease 22.34%, 46.43% and 49.17%, respectively. The results showed that the corn protein polypeptide showed a dual effect, low doses corn protein polypeptide （100 mg/kg diet） added for longer time in the diet of geese could effectively promote the FAS expressing in livers, reduce the FAS expressing in adipose tissue, so as to facilitate the regulation of the body lipid metabolism, improve the quality of carcasses.

Key words: corn protein polypeptide; immunohistochemical method; fatty acid synthetase; geese

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