不同免疫增效剂对鸡新城疫免疫效果的影响

›› 2011, Vol. 38 ›› Issue (1): 188-190.

不同免疫增效剂对鸡新城疫免疫效果的影响

李富桂1,张文生2,梁庆欣1,赵丽敏1,王志龙1

(1.天津农学院动物科学系，天津 300384；2.天津职业大学生物与环境工程学院，天津 300402)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-01-01 发布日期:2011-01-01

Effect on Immune Efficiency of Newcastle Disease with Different Immune Intensifiers

LI Fu-gui1, ZHANG Wen-sheng2, LIANG Qing-xin1， ZHAO Li-min1， WANG Zhi-long1

（1.Department of Animal Science, Tianjin Agricultural College, Tianjin 300384,China;2.Institute of Biology and Environment Engineering,Tianjin Vocational Institute, Tianjin 300402, China)

Received:1900-01-01 Revised:1900-01-01 Online:2011-01-01 Published:2011-01-01

摘要/Abstract

摘要：

为研究鸡转移因子、黄芪、左旋咪唑、灵芝4种免疫增效剂的功效，以鸡新城疫（Newcastle disease，ND）血凝抑制抗体效价作为指标，比较其免疫效果。试验鸡先采血测定ND抗体效价，接种注射ND-IB-EDS灭活油乳剂疫苗，再将试验鸡随机分成5组，1～4组为试验组，给予不同的免疫增效剂，5组为空白对照组。在给药后第4、6、8、10、12、14、16、18、20、22和24天分别采血，采用微量血凝抑制试验检测ND抗体效价，统计后比较其抗体效价的变化。结果表明：灵芝组抗体效价在12 d开始显著高于对照组和其他试验组（P＜0.05），且保持较高的水平；转移因子组和黄芪组抗体效价从14 d开始均显著高于对照组和左旋咪唑组（P＜0.05）,并分别从第22和20天开始持续在较高水平；左旋咪唑组的抗体效价高于对照组，但未出现显著性差异。由此可见,不同的免疫增效剂对鸡ND疫苗的免疫增强作用各异。综合考虑，临床应用时首选鸡转移因子，其次黄芪，灵芝和左旋咪唑需酌情使用。

关键词: 免疫增效剂; 免疫效果; 新城疫抗体效价; 鸡转移因子; 黄芪; 左旋咪唑; 灵芝

Abstract: Immune effects of 4 different immune intensifiers（chicken transfer factor, astragalus, levamisole and ganoderma）were compared in this paper using Newcastle disease (ND) antibody titre as indicator. Blood samples were collected for detecting ND HI titre before experiment, ND-EDS-IB attenuated oil-emulsion vaccine was injected into each bird. And then all chickens were divided into five groups，group 1 to 4 were experimental groups and group 5 was control，and all birds were given with different immune intensifiers. Then, antibody titres of blood samples collected on 4th,6th, 8th, 10th, 12th, 14th, 16th, 18th, 20th, 22th, 24th day post-immunization were detected by HI test, changes of HI titres were received by mathematical statistics. The results showed that HI titre of ganoderma group were significant higher than control group and other groups at 12th day firstly,and kept in high level; there were more distinct enhancement respectively in transfer factor group and astragalus group in 14th day, keeping in high level;the titre of levamisole group was higher than control group after 20th day, but there was no significant differentiation. Therefore, there were various immune efficiencies to ND vaccine with different immune intensifiers. By comprehensive consideration, chicken transfer factor (priority) and astragalus (secondary choice) would be applied in clinic, and ganoderma and levamisole should be used appropriately.

Key words: immune intensifier; immune efficiency; Newcastle disease antibody titre; chicken transfer factor; astragalus; levamisole; ganoderma

中图分类号:

S852.4

李富桂;张文生;梁庆欣;赵丽敏;王志龙. 不同免疫增效剂对鸡新城疫免疫效果的影响[J]. , 2011, 38(1): 188-190.

LI Fu-gui;ZHANG Wen-sheng;LIANG Qing-xin;ZHAO Li-min;WANG Zhi-long. Effect on Immune Efficiency of Newcastle Disease with Different Immune Intensifiers[J]. , 2011, 38(1): 188-190.

[1]	卢思嘉, 郑兰兰. 猪流行性腹泻病毒疫苗研究进展[J]. 中国畜牧兽医, 2023, 50(7): 2931-2940.
[2]	刘馨, 潘吉脉, 胡安东, 曾成容, 杨颖, 程振涛, 陈江凤, 姜海波, 文明. 黄芪多糖对杂交鲟用嗜水气单胞菌灭活疫苗的影响[J]. 中国畜牧兽医, 2023, 50(3): 1177-1184.
[3]	林厦菁, 苟钟勇, 范秋丽, 叶金玲, 阮栋, 王一冰, 张赛, 潘木水, 蒋守群. 黄芪多糖对黄羽肉种母鸡繁殖性能和抗氧化能力的影响[J]. 中国畜牧兽医, 2023, 50(12): 4837-4845.
[4]	刘颖, 田旭, 冯晓梦, 吕晓萍, 高雪丽, 郑世民, 刘超男. 黄芪多糖对肠炎雏鸡小肠黏膜损伤的保护作用[J]. 中国畜牧兽医, 2023, 50(1): 76-85.
[5]	孙悦龙, 张梦洁, 豆佳红, 王小莹, 戴小枫, 李秀梅. 基于网络药理学及分子对接技术研究黄芪调控仔猪肠道菌群失调性腹泻的作用[J]. 中国畜牧兽医, 2022, 49(8): 3200-3211.
[6]	刘倩, 郭志廷, 张康, 王学智, 张景艳, 李建喜. 黄芪多糖对巨噬细胞天然免疫调节的研究进展[J]. 中国畜牧兽医, 2022, 49(5): 1746-1756.
[7]	徐丽云, 郑梦荷, 许子豪, 牛高伟, 王永力, 邱辉, 吴宇轩, 金桐, 邵伟. 灌服黄芪多糖对奶牛产奶量、免疫性能及抗氧化性能的影响[J]. 中国畜牧兽医, 2022, 49(10): 3819-3828.
[8]	田全华, 赵旭, 刘勇, 魏凌云, 谭支良, 贺志雄. 饲粮添加黄芪和葡聚糖硫酸钠盐刺激对羔羊后肠道发酵和微生物组成的影响[J]. 中国畜牧兽医, 2021, 48(9): 3242-3253.
[9]	乔宏兴, 张立恒, 张晓静, 宋予震, 边传周. 基于LC-MS代谢组学的植物乳杆菌发酵黄芪的代谢产物分析[J]. 中国畜牧兽医, 2021, 48(9): 3283-3292.
[10]	陶新磊, 刘丹华, 田旭, 郑世民, 高雪丽, 吕晓萍, 刘超男. 黄芪多糖诱导SOCS3表达对鸡巨噬细胞炎症反应的抑制作用[J]. 中国畜牧兽医, 2021, 48(11): 4284-4291.
[11]	刘巧玲, 黄涛, 汤德元, 曾智勇, 石柯, 林泠, 任杰. 乙型脑炎病毒NS1和NS1-2A蛋白的真核表达及其差异性研究[J]. 中国畜牧兽医, 2020, 47(7): 2190-2199.
[12]	闫宗斌, 伍生军, 杨静, 闫可心, 相思宇, 许应天, 薛书江, 柴方红. 猪附红细胞体eno基因重组蛋白的制备及免疫效果研究[J]. 中国畜牧兽医, 2020, 47(7): 2231-2238.
[13]	潘悦, 曹旗, 任洪林, 胡盼, 李岩松, 周玉, 柳增善, 卢士英. 鼠源CD40L的原核表达及其对河豚毒素半抗原免疫增强效果分析[J]. 《中国畜牧兽医》, 2019, 46(8): 2438-2445.
[14]	闫可心, 伍生军, 赵云, 王淼, 许应天, 薛书江. 表达猪附红细胞体ENO基因重组腺病毒的构建及免疫原性分析[J]. 《中国畜牧兽医》, 2019, 46(7): 2088-2095.
[15]	李明, 张雅娜, 郭晓宇, 高新桃, 李金祥, 朱鸿飞. 口蹄疫疫苗非抗原蛋白对146S抗原免疫效果的影响[J]. 《中国畜牧兽医》, 2019, 46(3): 905-912.