›› 2010, Vol. 37 ›› Issue (4): 174-177.

• 奶业专栏 • 上一篇    下一篇

子宫内膜炎奶牛产后氧化损伤与抗氧化变化

李德军1,2,郭定宗2,裴小英2

  

  1. (1.东北农业大学动物医学学院, 哈尔滨 150030; 2.华中农业大学动物医学院, 武汉 430070)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-04-20 发布日期:2010-04-20
  • 通讯作者: 郭定宗

Oxidative Damage and Changes of Antioxidant Defense Capability in Postpartum Endometritis Cows

LI De-jun1,2, GUO Ding-zong2,PEI Xiao-ying2   

  1. (1.College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; 2.College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-04-20 Published:2010-04-20
  • Contact: GUO Ding-zong

摘要: 通过对试验用荷斯坦奶牛全血中白细胞总数(WBC)、中性粒细胞(PMN)百分比变化及血清、子宫分泌物中的一氧化氮(NO)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)的活性进行检测,探讨产后患有不同程度子宫内膜炎奶牛的氧化损伤和抗氧化能力的变化及其机理。结果表明,CE组和SE组全血中WBC总数和PMN百分比显著高于对照组(P<0.01,P<0.05)。而CE组血清和子宫分泌物中NO、MDA含量明显高于N组(P<0.01),SE组则变化不明显,CE组SOD和GSH-Px活性变化比SE组明显,表现差异显著(P<0.01,P<0.05)。试验证明,子宫内膜炎奶牛血清和子宫分泌物中脂质过氧化物含量升高、抗氧化能力下降,可能是子宫内组织细胞病理损伤,引起不同程度子宫内膜炎的重要原因之一。

关键词: 荷斯坦奶牛; 子宫内膜炎; 氧化损伤; 抗氧化功能

Abstract: To study the oxidative damage and the antioxidant status and mechanisms of them in cows with varying degrees endometritis. The content of nitric oxide(NO), malonaldehyde(MDA)and the activity of superoxide dismutase(SOD), glutathione peroxidase(GSH-Px) in serum and uterine secretions of endometritis cows were detected. The total white blood cells and percent of PMN in CE and SE were significantly higher than the control(P<0.01, P<0.05). The content of NO and MDA in serum and uterine secretions of clinical endometritis cows was significantly higher than the control (P<0.01),but subclinical endometritis was not different, and the activity of SOD and GSH-Px with clinical endometritis was significantly lower than control group (P<0.01,P<0.05). It could be concluded that the increasing of free radicals and lipid peroxide and the imbalance of antioxidant defense system in serum and uterine secretions of endometritis cows was one reason of cytopathological damage in uterine tissue.

Key words: Holstein cow; endometritis; oxidative damage; antioxidant capability

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