›› 2009, Vol. 36 ›› Issue (8): 47-48.

• 生物技术 • 上一篇    下一篇

藏猪肥胖基因多态性研究

张浩1,强巴央宗2,彭俊飞1,杨涛2,达娃卓玛2,吴常信1   

  1. (1.中国农业大学动物科技学院, 北京 100193; 2.西藏农牧学院动物科学系, 林芝 860000)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-08-20 发布日期:2009-08-20

Polymorphisms of Obesity Gene in Tibet Pigs

ZHANG Hao1, Chambayangzom2, PENG Jun-fei1, YANG Tao2, Dawazhuoma2, WU Chang-xin1   

  1. (1.College of Animal Science and Technology, China Agricultural University, Bejing 100193, China;2.College of Agriculture and Animal Husbandry, Tibet University, Linzhi 860000, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-08-20 Published:2009-08-20

摘要: 藏猪为中国高原特色的瘦肉型猪种,本研究采用PCR-RFLP技术检测藏猪肥胖基因(ob)外显子2内Hinf I酶切多态性。结果显示,藏猪群体内出现TT、CT和CC 3种基因型,其中突变型CC频率最高,为50.00%;TT基因型频率最低,为6.25%;等位基因T和C频率分别为28.12%和71.88%。其频率分布处于瘦肉型外种猪和脂肪性地方猪之间,但更接近脂肪性猪,说明从该位点看藏猪并不属于瘦肉型猪种,其瘦肉率较高可能与营养和饲养环境等有关。

关键词: 藏猪; ob基因; RFLP; 多态性

Abstract: Tibet pig is a special Chinese indigenous pig breed native to high-altitude areas of Tibet. In the present study, the polymorphism of exon2 of obesity gene was measured with the method of PCR-Hinf I-RFLP in Tibet pig. The results showed that there were three genotypes, TT, CT and CC, in Tibet pig population, and the predominant genotypes was CC (50%). The frequencies of allele T and allele C were 28.12% and 71.88% respectively. The distribution of genotype in Tibet pig population was between lean-type pigs and fat-type pigs, which indicated that according to this locus Tibet pig doesn’t belong to lean-type breeds, and its high lean percentage may relate to nutrition and environment.

Key words: Tibet pig; obesity gene; RFLP; polymorphism

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