迪庆藏猪与野藏杂交猪肌肉全谱游离氨基酸味道强度值比较

doi:10.16431/j.cnki.1671-7236.2021.04.014

中国畜牧兽医 ›› 2021, Vol. 48 ›› Issue (4): 1275-1283.doi: 10.16431/j.cnki.1671-7236.2021.04.014

迪庆藏猪与野藏杂交猪肌肉全谱游离氨基酸味道强度值比较

马黎¹, 聂靖茹^2,3, 鲁绍雄^2,3, 刘诗意^2,3, 王琳^2,3, 李金恬^2,3, 严达伟^2,3, 董新星²

1. 云南农业职业技术学院, 昆明 650212;
2. 云南农业大学动物科学技术学院, 昆明 650201;
3. 云南省动物营养与饲料重点实验室, 昆明 650201

修回日期:2020-09-23 出版日期:2021-04-20 发布日期:2021-04-16
通讯作者: 严达伟, 董新星 E-mail:1302648630@qq.com;86127447@qq.com
作者简介:马黎(1971-),女,云南个旧人,硕士,副教授,研究方向:动物营养与饲料科学,E-mail:935936939@qq.com
基金资助:
国家重点研发计划项目（2018YFD0501200）；云南省农业联合重点项目（2018FG001-003）；云南省重大专项计划项目（2018BB003）

Comparison of the Taste Activity Value of Full Spectrum Free Amino Acid in Muscle of Diqing Tibetan Pig and Wild Boar×Diqing Tibetan Pig

MA Li¹, NIE Jingru^2,3, LU Shaoxiong^2,3, LIU Shiyi^2,3, WANG Lin^2,3, LI Jintian^2,3, YAN Dawei^2,3, DONG Xinxing²

1. Yunnan Vocational and Technical College of Agriculture, Kunming 650212, China;
2. College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China;
3. The Key Laboratory of Nutrition and Feed of Yunnan Province, Kunming 650201, China

Revised:2020-09-23 Online:2021-04-20 Published:2021-04-16

摘要/Abstract

摘要： 本研究旨在探明迪庆藏猪与野猪×迪庆藏猪（野藏杂交猪）肌肉全谱游离氨基酸（FAA）味道强度值（TAV）的差异。选择胎次相同、出生日期相近、体重20 kg左右的迪庆藏猪和野藏杂交猪各12头（公、母各半），以迪庆藏猪为对照，采用相同饲粮在相同饲养条件下直线育肥，100 kg左右屠宰，采集背最长肌，沸水蒸30 min后，用高效液相色谱-四级杆离子阱串联质谱仪检测其全谱游离氨基酸，计算呈味氨基酸TAV并比较其差异。结果显示，与迪庆藏猪相比，野藏杂交猪肌肉游离氨基酸总鲜味TAV降低16.27%（P<0.05），总甜味、酸味和苦味TAV分别降低3.50%、11.68%和1.50%，但差异均不显著（P>0.05）；野藏杂交猪肌肉谷氨酰胺（Gln）鲜味TAV、甜味TAV均降低22.89%（P<0.05），缬氨酸（Val）甜味、苦味TAV均增加36.32%（P<0.05）；天冬氨酸（Asp）、甘氨酸（Gly）、谷氨酸（Glu）与组氨酸（His）等其他游离氨基酸的鲜味、甜味、酸味和苦味TAV与迪庆藏猪间均差异不显著（P>0.05）。以上结果表明，与野猪杂交会降低迪庆藏猪肌肉鲜味TAV，降低Gln的鲜味、甜味TAV，提高Val甜味、苦味TAV，该结果可为迪庆藏猪的开发利用提供依据。

关键词: 迪庆藏猪; 野藏杂交猪; 全谱游离氨基酸; 味道强度值

Abstract: This experiment was conducted to study the difference in the taste activity value (TAV) of the full spectrum of free amino acids (FAA) in the muscles of Diqing Tibetan pigs(TP) and its hybrid wild boars (WT).12 TPs and 12 WTs with the same parity,similar birth dates,and weighing about 20 kg were selected and fed with the same feed,and then slaughtered when they reached about 100 kg.The longissimus dorsi (LD) was collected and steamed with boiling water for 30 min.Taking TPs as control,the composition and content of FAA were detected by high performance liquid chromatography-quadruple/linear ion trap mass spectrometry (HPLC-Q-Trap-MS),then the differences in TAV of FAA were calculated and compared.The results showed that compared with TPs,the total umami TAV of FAA in WTs was decreased by 16.27% (P<0.05).The total sweet,sour and bitter TAV of FAA in WTs was decreased by 3.50%,11.68% and 1.50% respectively,but the difference was not significant (P>0.05).The umami and sweetness TAV of glutamine (Gln) in WTs were both decreased by 22.89% (P<0.05),and the sweetness and bitterness TAV of valine (Val) were both increased by 36.32%(P<0.05).There was no significant difference in the umami,sweet,sour and bitter TAV of other amino acids such as aspartic acid,glycine,glutamic acid and histidine between the two groups (P>0.05).In conclusion,crossbreeding with wild boars would reduce the umami TAV of TP,the umami and sweetness TAV of Gln,and increase the sweetness and bitterness TAV of Val,which could provide basis for the development and utilization of TP.

Key words: Diqing Tibetan pig; wild and Diqing Tibetan hybrid pig; full spectrum free amino acid; taste activity value

中图分类号:

S828

马黎, 聂靖茹, 鲁绍雄, 刘诗意, 王琳, 李金恬, 严达伟, 董新星. 迪庆藏猪与野藏杂交猪肌肉全谱游离氨基酸味道强度值比较[J]. 中国畜牧兽医, 2021, 48(4): 1275-1283.

MA Li, NIE Jingru, LU Shaoxiong, LIU Shiyi, WANG Lin, LI Jintian, YAN Dawei, DONG Xinxing. Comparison of the Taste Activity Value of Full Spectrum Free Amino Acid in Muscle of Diqing Tibetan Pig and Wild Boar×Diqing Tibetan Pig[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1275-1283.

参考文献

[1] 李建军,文杰.肉滋味研究进展[J].食品科技,2001,5:27-28. LI J J,WEN J.Development of research on the taste of poultry meat[J].Food Science and Technology,2001,5:27-30.(in Chinese)
[2] 蒋滢,徐颖,朱庚伯.人类味觉与氨基酸味道[J].氨基酸和生物资源,2002,24(4):70. JIANG Y,XU Y,ZHU G B.Human taste and amino acid taste[J].Amino Acids and Biotic Resources,2002,24(4):70.(in Chinese)
[3] DASHDORJ D,AMNA T,HWANG I.Influence of specific taste-active components on meat flavor as affected by intrinsic and extrinsic factors:An overview[J].European Food Research and Technology,2015,241(2):157-171.
[4] TOLDRA F.Proteolysis and lipolysis in flavor development of dry-cured meat products[J].Meat Science,1998,49(suppl 1):101-110.
[5] TOLDRA F,ARISTOY M C,FLORES M.Contribution of muscle aminopeptidases to flavor development in dry-cured ham[J].Food Research International,2000,33(3):181-185.
[6] 杨平,王瑶,宋焕禄,等.不同熬制条件下猪肉汤中滋味成分的变化[J].中国食品学报,2018,18(2):247-260. YANG P,WANG Y,SONG H L,et al.Analysis of non-volatile flavor components in pork broth with different cooking conditions[J].Journal of Chinese Institute of Food Science and Technology,2018,18(2):247-260.(in Chinese)
[7] 马菲,郇延军,刁欣悦.酱制时间对传统酱卤猪肉制品风味及质构变化规律的影响[J].食品与机械,2019,35(9):55-64. MA F,HUAN Y J,DIAO X Y.Effect of sauce time on the flavor and texture change of traditional sauce and braised pork products[J].Food and Machinery,2019,35(9):55-64.(in Chinese)
[8] 严达伟,赵桂英,苟潇,等.迪庆藏猪肉质特性的研究[J].云南农业大学学报,2007,22(1):86-91. YAN D W,ZHAO G Y,GOU X,et al.Study on characteristics of Diqing Tibetan pig's meat quality[J].Journal of Yunnan Agricultural University,2007,22(1):86-91.(in Chinese)
[9] 云南省畜牧局,云南省家畜家禽品种志编写委员会.云南省家畜家禽品种志[M].昆明:云南科技出版社,1987. YUNNAN PROVINCIAL ANIMAL HUSBANDRY BUREAU.YUNNAN PROVINCIA LIVESTOCK AND POULTRY BREEDING COMMITTEE.Yunnan Livestock and Poultry Breeding Committee[M].Kunming:Yunnan Science and Technology Press,1987.(in Chinese)
[10] 滚双宝.野猪种群特性及其生态作用的研究进展[J].特产研究,2004,1:52-56. GUN S B.Research progress on population character and ecological function of wild swine[J].Special Wild Economic Animal and Plant Research,2004,1:52-56.(in Chinese)
[11] 严艳伶,王怡敏,张雁辉,等.云南省野猪人工繁育产业调查评估[J].林业调查规划,2019,44(2):144-146. YAN Y L,WANG Y M,ZHANG Y H,et al.Investigation and evaluation on artificial breeding industry of wild boar in Yunnan province[J].Forest Invenntory and Planning,2019,44(2):144-146.(in Chinese)
[12] 陈国顺.子午岭野家杂种猪和合作猪肉质特性比较及风味挥发性成分的提取与分析[D].兰州:甘肃农业大学,2004. CHEN G S.Comparison of meat quality and analysis of flavor chemical components of crossbred pigs bred by Sus scrofa from Ziwuling mountain and Hezuo pig[D].Lanzhou:Gansu Agricultural University,2004.(in Chinese)
[13] 蔡原,赵有璋,蒋玉梅,等.顶空固相微萃取-气-质联用检测合作猪肉挥发性风味成分[J].西北师范大学学报(自然科学版),2006,42(4):74-78. CAI Y,ZHAO Y Z,JIANG Y M,et al.Determination of volatile compounds of Hezuo swine by GC-MS and headspace solid phase microextraction[J].Journal of Northwest Normal University (Natural Science),2006,42(4):74-78.(in Chinese)
[14] 李铁志,王明,雷激.阿坝州半野血藏猪肉挥发性风味物质的研究[J].食品科技,2015,40(10):134-140. LI T Z,WANG M,LEI J.Volatile flavor compounds of semi-wild-blood Aba Tibetan pork[J].Food Science and Technology,2015,40(10):134-140.(in Chinese)
[15] KIRIMURA J,SHIMIZU A,KIMIZUKA A,et al.Contribution of peptides and amino acids to the taste of foods[J].Journal of Agricultural and Food Chemistry,1969,17(4):689-695.
[16] CHEN D W,ZHANG M.Non-volatile taste active compounds in the meat of Chinese mitten crab (Eriocheir sinensis)[J].Food Chemistry,2007,104(3):1200-1205.
[17] SHALLENBERGER R S.Taste Chemistry[M].London:Blackie Academic and Professional,1993.
[18] ROTZOLL N,DUNKEL A,HOFMANN T.Quantitative studies,taste reconstitution and omission experiments on the key taste compounds in morel mushrooms(Morchella deliciosa,fr.)[J].Journal of Agricultural and Food Chemistry,2006,54(7):2705-2711.
[19] DALLA C F,DALLA C O,DI CASTRO I C,et al.Ease of handling and physiological parameters of stress,carcasses,and pork quality of pigs handled in different group sizes[J].Animals,2019,9(10):798-807.
[20] 高正义,王志祥,陈道付,等.谷氨酰胺的生理功能及其在幼龄动物营养中的应用[J].中国畜牧兽医,2007,34(8):16-20. GAO Z Y,WANG Z X,CHEN D F,et al.The physiological function of glutamine and its application in nutrition of young animals[J].Chinese Animal Husbandry & Veterinary Medicine,2007,34(8):16-20.(in Chinese)
[21] 杨大柳,徐琪寿.谷氨酰胺的药理作用[J].氨基酸和生物资源,1996,18(2):40-43. YANG D L,XU Q S.Pharmacology roles of glutamine[J].Amino Acids and Biotic Resources,1996,18(2):40-43.(in Chinese)
[22] 黄耀凌,都思湘.谷氨酰胺的抗疲劳生化机制研究[J].南京农业大学学报,2001,24(2):87-89. HUANG Y L,DOU S X.Effect and biomechanism of glutamine on fatigue resistance[J].Journal of Nanjing Agricultural University,2001,24(2):87-89.(in Chinese)
[23] 李德发.猪的营养[M].北京:中国农业科学技术出版社,2003. LI D F.Pig Nutrition[M].Beijing:China Agricultural Science and Technology Press,2003.(in Chinese)
[24] 张涛.猪饲粮中缬氨酸的营养研究进展[J].饲料与畜牧,2018,369(12):70-74. ZHANG T.Research progress of valine nutrition in pig diets[J].Feed and Animal Husbandry,2018,369(12):67-71.(in Chinese)
[25] 朱洪强,王全凯,殷树鹏.野猪肉与家猪肉营养成分的比较分析[J].西北农业学报,2007,16(3):54-56. ZHU H Q,WANG Q K,YIN S P.The comparison analysis of the boar meat and pork in nourishment composition[J].Acta Agriculturae Boreali-occidentalis Sinica,2007,16(3):54-56.(in Chinese)
[26] WANG R,YANG C,SONG H L.Key meat flavour compounds formation mechanism in a glutathione-xylose maillard reaction[J].Food Chemistry,2012,131(1):280-295.
[27] 王曜,陈舜胜.野生与养殖克氏原螯虾游离氨基酸的组成及比较研究[J].食品科学,2014,35(11):269-273. WANG Y,CHEN S S.Comparative composition of free amino acids in wild and cultured Procambarus clarkii[J].Food Science,2014,35(11):269-273.(in Chinese)
[28] ROTOLA-PUKKILA M K,PIHLAJAVⅡTA S T,KAIMAINEN M T,et a1.Concentration of umami compounds in pork meat and cooking juice with different cooking times and temperatures[J].Journal of Food Science,2015,80(12):C2711-6.
[29] 齐晓园,李明丽,马黎,等.育肥方式对滇南小耳猪肌肉氨基酸的影响[J].家畜生态学报,2017,38(4):25-32. QI X Y,LI M L,MA L,et al.Impact of different fattening ways on amino acids in muscles of Diannan Small-ear pig[J].Acta Ecologiae Animalis Domastici,2017,38(4):25-32.(in Chinese)
[30] 杨公社,高整团,刘艳芬,等.八眉猪肉脂品质研究[J].中国农业科学,1994,27(5):63-68. YANG G S,GAO Z T,LIU Y F,et al.Studies on the meat quality of Bamei pigs[J].Scientia Agricultura Sinica,1994,27(5):63-68.
[31] 朱砺,李学伟,帅素容,等.大河猪与大河乌猪的肌肉营养成分分析[J].中国畜牧杂志,2008,44(7):6-9. ZHU L,LI X W,SHUAI S R,et al.Analysis of muscle nutritional components of Dahe pig and Dahe Black pig[J].Chinese Journal of Animal Science,2008,44(7):6-9.(in Chinese)
[32] HIROMICHI K,MEE R R,TOSHIHIDE N.Role of Free Amino Acids and Peptides in Food Taste[M].Washington:ACS Symposium Series,1989.
[33] 常亚楠,赵改名,柳艳霞,等.煮制对鸡肉及汤汁中游离氨基酸的影响[J].食品工业科技,2014,35(9):333-337. CHANG Y N,ZHAO G M,LIU Y X,et al.Changes of free amino acids in chicken and its broth during cooking[J].Science and Technology of Food Industry,2014,35(9):333-337.(in Chinese)

迪庆藏猪与野藏杂交猪肌肉全谱游离氨基酸味道强度值比较

Comparison of the Taste Activity Value of Full Spectrum Free Amino Acid in Muscle of Diqing Tibetan Pig and Wild Boar×Diqing Tibetan Pig

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