猪IGFBP2基因遗传多态性及其与肉质性状的关联分析

doi:10.16431/j.cnki.1671-7236.2017.10.026

摘要/Abstract

摘要：

试验旨在探究胰岛素样生长因子结合蛋白2（insulin-like growth factor binding protein 2，IGFBP2）基因遗传变异对猪肉质性状的影响，为猪品种选育提供遗传学依据。参考GenBank中猪IGFBP2基因序列（登录号：BV727778）设计1对引物，采用PCR-RFLP技术检测IGFBP2基因在苏姜猪、姜曲海猪、长白猪中的遗传多态性，并分析基因座多态性与猪肉质性状的相关性。结果发现，3个猪种IGFBP2基因内含子3均具有Msp Ⅰ酶切多态性，分别检测到3种基因型：AA、AB和BB；χ²适合性检验显示，3个猪种IGFBP2基因基因型分布均处于Hardy-Weinberg平衡状态（P > 0.05）；多态信息含量（PIC）分析显示，3个猪种均为中度多态。IGFBP2基因不同基因型与肉质性状关联分析显示，3个猪种不同基因型肉色、肌内脂肪含量、大理石纹差异显著，AA基因型肉色（姜曲海猪除外）、肌内脂肪含量均显著高于AB、BB基因型（P < 0.05）；AA、AB基因型大理石纹显著高于BB基因型（P < 0.05），其余指标间差异不显著（P > 0.05）。综上，苏姜猪、姜曲海猪、长白猪IGFBP2基因内含子3多态性对肉质性状影响显著，可作为潜在影响猪肉质性状的候选基因。

关键词: 猪; IGFBP2基因; PCR-RFLP; 肉质性状

Abstract:

This study was aimed to analyze the genetic variation of insulin-like growth factor binding protein 2 (IGFBP2) gene effect on meat quality traits in pigs. A pair of primer was designed,which based on DNA sequence of IGFBP2 gene (GenBank accession No.:BV727778). The genetic polymorphism of IGFBP2 gene was detected by PCR-RFLP in Sujiang pig, Jiangquhai pig and Landrace pig, and the association between IGFBP2 gene polymorphism and meat quality traits in three pig breeds was analyzed. The results showed that three genotypes (AA, AB and BB) were detected. The Chi-square test results showed that the genotype distribution of three pig breeds was in Hardy-Weinberg equilibrium (P > 0.05). The polymorphism information content (PIC) were moderately polymorphic in three pig breeds. According to the association analysis of different genotypes, significant difference was found in color value, intramuscular fat content and marbling, the color value (except for Jianquhai pig) and intramuscular fat content of AA genotype were significantly higher than AB and BB genotypes (P < 0.05), the marbling of AA and AB genotypes was significantly higher than BB genotype (P < 0.05). In conclusion,the polymorphism of IGFBP2 gene intron 3 had significant effects on meat quality traits, and was candidate gene for the potential impact on meat quality traits of pigs.

Key words: pigs; IGFBP2 gene; PCR-RFLP; meat quality traits

中图分类号:

S813.3

刘海霞, 朱爱文. 猪IGFBP2基因遗传多态性及其与肉质性状的关联分析[J]. 《中国畜牧兽医》, 2017, 44(10): 3016-3022.

LIU Hai-xia, ZHU Ai-wen. Polymorphism of IGFBP2 Gene and Its Association with Meat Quality Traits in Pigs[J]. , 2017, 44(10): 3016-3022.

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