不同品种猪感染HP-PRRSV后肺组织间PRRSV受体基因差异表达的研究

doi:10.16431/j.cnki.1671-7236.2017.02.033

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (2): 538-545.doi: 10.16431/j.cnki.1671-7236.2017.02.033

不同品种猪感染HP-PRRSV后肺组织间PRRSV受体基因差异表达的研究

康润敏¹, 姬高升², 曾凯¹, 吕学斌¹, 殷明郁¹

1. 四川省畜牧科学研究院, 动物遗传育种四川省重点实验室, 成都 610066;
2. 四川大学生命科学学院, 动物疫病防控与食品安全四川省重点实验室, 生物资源与生态环境教育部重点实验室, 成都 610064

收稿日期:2016-08-12 出版日期:2017-02-20 发布日期:2017-02-25
通讯作者: 殷明郁 E-mail:10999042@qq.com
作者简介:康润敏(1983-), 女, 四川雅安人, 博士, 研究方向:动物疫病防控, E-mail:78157881@qq.com
基金资助:
四川公益性科研院所基本科研项目（SASA2015A03）；四川省科技支撑计划（2014NZ009、16ZC2850）；国家生猪产业技术体系（CARS-36）

Study on Differential Expression of PRRSV Receptor Genes in Lung Tissues of Different Breeds of Pig Infected with HP-PRRSV

KANG Run-min¹, JI Gao-sheng², ZENG Kai¹, LV Xue-bin¹, YIN Ming-yu¹

1. Animal Breeding and Genetics Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu 610066, China;
2. Key Laboratory of Bio-resources and Ecoenvironment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu 610064, China

Received:2016-08-12 Online:2017-02-20 Published:2017-02-25

摘要/Abstract

摘要：

为进一步了解藏猪、藏梅猪和大约克夏猪对高致病性猪繁殖与呼吸综合征病毒（HP-PRRSV） JXA1分离株具有不同易感性的分子机理，本研究建立实时荧光定量RT-PCR方法比较分析藏猪、藏梅猪和大约克夏猪感染JXA1分离株前、感染后4、7和14 d肺脏中HSPG2、SIGLEC1、CD163、VIM和NMMHC-ⅡA 5个PRRSV受体基因的差异表达情况。结果显示，藏猪感染JXA1分离株后4和14 d肺脏中HSPG2的表达量显著高于感染前（P < 0.05），而感染后7 d HSPG2的表达量显著低于感染前（P < 0.05），藏梅猪在感染后14 d HSPG2的表达量显著高于感染后7 d（P < 0.05）；藏猪感染后4和14 d肺脏中SIGLEC1的表达量显著高于感染前（P < 0.05），大约克夏猪感染后4、7和14 d SIGLEC1的表达量均显著低于感染前（P < 0.05）；藏猪和藏梅猪感染后14 d肺脏中CD163的表达量均显著高于感染前（P < 0.05），大约克夏猪则感染后7和14 d均显著低于感染前（P < 0.05）；藏猪感染后7 d肺脏中VIM的表达量显著高于感染前（P < 0.05），大约克夏猪在感染后7 d显著低于感染前（P < 0.05）；藏梅猪感染后4 d肺脏中NMMHC-ⅡA的表达量显著高于感染前（P < 0.05），大约克夏猪在感染后4和14 d NMMHC-ⅡA的表达量显著高于感染前（P < 0.05）。结果表明，5个PRRSV受体基因中，SIGLEC1和VIM基因可能是影响藏猪、藏梅猪和大约克夏猪对JXA1分离株易感性存在差异的潜在关键基因。

关键词: JXA1分离株; 藏猪; 藏梅猪; 大约克夏猪; PRRSV受体基因; 差异表达

Abstract:

In order to understand the molecular mechanism of host susceptibility differential to highly pathogenic porcine reproductive and respiratory syndrome (HP-PRRSV) infection among three breeds of pig, Tibetan pig,ZangMei Black and Yorkshire,the mRNA level of five reported PRRSV receptor genes (HSPG2,SIGLEC1,CD163,VIM and NMMHC-ⅡA) expressed in lung were investigated on 0,4, 7 and 14 days post infection (dpi) with HP-PRRSV JXA1 isolate.The Real-time RT-PCR results showed that the expression of HSPG2 in Tibetan pig increased significantly on 4 and 14 dpi and decreased significantly on 7 dpi compared with the expression on 0 dpi (P < 0.05), and its expression in ZangMei Black on 14 dpi was significantly higher than that of on 7 dpi (P < 0.05);An increased expression of SIGLEC1 in Tibetan pig was observed on 4 and 14 dpi (P < 0.05), and a decreased expression of the same gene was detected in Yorkshire on 4,7 and 14 dpi (P < 0.05);The expression of CD163 elevated significantly on 14 dpi in both Tibetan pig and ZangMei Black (P < 0.05), however its expression reduced significantly on 7 and 14 dpi in Yorkshire (P < 0.05); The expression of VIM gene was significantly higher on 7 dpi in Tibetan pig and significantly lower on 7 dpi in Yorkshire (P < 0.05); And the expression of NMMHC-ⅡA was significantly higher on 4 dpi in ZangMei Black, which was also significantly higher in Yorkshire on 4 and 14 dpi (P < 0.05).In conclusion, SIGLEC1 and VIM might be important genes to influence the host resistance of Tibetan pig,ZangMei Black and Yorkshire to HP-PRRSV infection.

Key words: JXA1 isolate; Tibetan pig; ZangMei Black; Yorkshire; PRRSV receptor genes; differential expression

中图分类号:

S855.3

康润敏, 姬高升, 曾凯, 吕学斌, 殷明郁. 不同品种猪感染HP-PRRSV后肺组织间PRRSV受体基因差异表达的研究[J]. 《中国畜牧兽医》, 2017, 44(2): 538-545.

KANG Run-min, JI Gao-sheng, ZENG Kai, LV Xue-bin, YIN Ming-yu. Study on Differential Expression of PRRSV Receptor Genes in Lung Tissues of Different Breeds of Pig Infected with HP-PRRSV[J]. , 2017, 44(2): 538-545.

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不同品种猪感染HP-PRRSV后肺组织间PRRSV受体基因差异表达的研究

Study on Differential Expression of PRRSV Receptor Genes in Lung Tissues of Different Breeds of Pig Infected with HP-PRRSV

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