›› 2009, Vol. 36 ›› Issue (1): 145-147.

• 经验交流 • 上一篇    下一篇

耐酸性α-淀粉酶产生菌发酵条件的优化

洪新,唐克   

  1. 辽宁工业大学材料与化学工程学院,锦州 121001
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-01-20 发布日期:2009-01-20

Study on Fermentative Condition of the Acid Resistant Alpha-amylase Producing Strains

HONG Xin,TANG Ke   

  1. School of Material and Chemical Engineering, Liaoning Institute of Technology, Jinzhou 121001, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-01-20 Published:2009-01-20

摘要: 试验对从淀粉厂附近的土壤中筛选的产耐酸性α-淀粉酶的菌株发酵条件进行了考察,确定最佳发酵条件为pH 4.5、碳源为可溶性淀粉、氮源为柠檬酸二氢铵、培养温度为35 ℃、接种量为2 mL。 

关键词: 发酵; 淀粉酶; 酶活

Abstract: Fermentative condition of the acid resistant alphaamylase producing strain which was isolated from the soil sample was studied, the optimum conditions are as fallows: pH is 4.5,carbon resource is soluble starch, nitrogen resource is dihydroammonium citrate, culturing temperature is 35 ℃,inoculation quantum is 2 mL.

Key words: ferment; amylase; enzyme activity

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