›› 2008, Vol. 1 ›› Issue (10): 47-50.

• 生物技术 • 上一篇    下一篇

猪H-FABP基因启动子区一个新SNP及其在几个猪种中的分布

额尔敦达古拉1,2,张龙超1,王立贤1,颜华1,道尔吉2
  

  1. 1.中国农业科学院北京畜牧兽医研究所,北京 100193; 2.内蒙古农业大学动物科学与医学学院,呼和浩特 010018
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-10-20 发布日期:2008-10-20

A New SNP in Porcine H-FABP Promoter Region and its Distribution in Several Pig Lines

Erdundagula1,2, ZHANG Longchao1, WANG Lixian1, YAN Hua1,Daoerji2
  

  1. 1. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;2. College of Animal Science and Veterinary, Inner Mongolia Agricultural University, Huhhot 010018, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-10-20 Published:2008-10-20

摘要: 通过设计3对引物,采用测序的方法研究猪H-FABP基因启动子区多态性发现,在该基因起始密码子ATG上游启动子区224 bp处存在1处新的突变位点(T-C),命名为H-FABP-proT224C。运用PCR-RFLP方法研究该位点在东北民猪、北京黑猪、长白猪、大白猪中的分布发现,只有肌内脂肪含量高的东北民猪中存在该突变,说明该突变位点很有可能与肌内脂肪含量相关。

关键词: 猪; H-FABP基因; 启动子区; SNP; 肌内脂肪含量

Abstract: Single nucleotide polymorphisms (SNPs) in H-FABP gene promoter region were detected in present study. A new SNP (T-C) was found at -224 bp, named H-FABP-proT224C, from the initiation codon (ATG) in promoter region. Distributions of H-FABP-proT224C in Min, Beijing Black, Landrace and Large White pigs were investigated. The SNP was found only in Min pig with more intramuscular fat content (IMF) provide the support that H-FABP-proT224C could play an important role on IMF. 

Key words: pig; H-FABP gene; promoter region; SNP; IMF

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