›› 2007, Vol. 34 ›› Issue (5): 67-69.

• 遗传繁育 • 上一篇    下一篇

藏猪SLA-DRB基因第2外显子PCR-RFLP多态性分析

鞠慧萍1,吴圣龙1,孙鹏翔1,包文斌1,2,许盛海1,李碧春1,陈国宏1
  

  1. 1.扬州大学动物科学与技术学院,扬州 225009; 2. 安徽农业大学动物科技学院,合肥 230036
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-05-20 发布日期:2007-05-20

Polymorphism Analysis of the Exon 2 of SLA-DRB Gene ofTibet Pig by PCR-RFLP

JU Huiping1,WU Shenglong1,SUN Pengxiang1,BAO Wenbin1,2,XU Shenghai1,LI Bichun1,CHEN Guohong1   

  1. 1.Animal Science and Technology College, Yangzhou University, Yangzhou 225009, China;  2. Animal Science and Technology, Anhui Agriculture University, Hefei 230036, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-05-20 Published:2007-05-20

摘要: 采用限制性内切酶RsaⅠ对藏猪SLA-DRB基因外显子2进行多态性分析。结果表明:藏猪SLA-DRB基因外显子2在RsaⅠ-RFLP位点上存在7种基因型,以杂合子BC型居多,基因型频率为0.4419,为优势基因型。χ2适合性检验结果表明,藏猪SLA-DRB基因外显子2 的RsaⅠ酶切位点的χ2值为10.14,高于显著水平(P<0.05),说明藏猪SLA-DRB基因在RsaⅠ酶切位点上没有达到Hardy-Weinberg平衡状态。

关键词: 藏猪; SLA-DRB基因; PCR-RFLP

Abstract: The exon 2 of SLA-DRB gene in Tibet pigs was analyzed by PCR-RFLP method. The results showed that the exon 2 digested by restriction endonuclease RsaⅠcould be divided into four kinds of alleles and seven kinds of genotypes in Tibet pigs respectively, and the frequency of BC was the highest(0.4419). The results of Chi Square test indicated that the frequencies of patterns digested by RsaⅠ were not fit in Hardy-Weinberg equilibrium in Tibet population(P<0.05).

Key words: tibet pig; SLA-DRB gene; PCR-RFLP

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