2023年成都地区猪圆环病毒3型流行病学调查及遗传进化分析

doi:10.16431/j.cnki.1671-7236.2024.10.033

摘要/Abstract

摘要： 【目的】调查分析成都地区猪圆环病毒3型(PCV3)的流行与分子遗传进化情况。【方法】试验于2023年1月至2023年12月从成都地区4家无害化处置场采集病死猪组织样品2 113份，采用实时荧光定量PCR法检测病料组织PCV3感染情况。设计3对引物对阳性样品的PCV3基因组序列进行PCR扩增并测序。通过在线软件拼接PCV3基因组序列、截取ORF2基因序列并推导其氨基酸序列(Cap)，进行相似性比对并构建遗传进化树，分析Cap氨基酸序列突变情况。【结果】组织病料检测结果显示，2 113份病死猪组织样品的PCV3阳性率为27.21%(575/2 113)。根据PCR扩增测序结果拼接获得了15株PCV3基因组序列。相似性比对分析显示，15株PCV3核苷酸序列相似性为97.2%～100%，ORF2基因核苷酸序列相似性为97.2%～100%，Cap氨基酸序列相似性为96.7%～100%。遗传进化树结果显示，12株PCV3属于PCV3a亚型，3株属于PCV3c亚型。15株Cap氨基酸序列中共13株发生了突变，共存在13个突变位点。【结论】成都地区PCV3主要为PCV3a和PCV3c亚型，基因组相似性高，序列较保守，Cap氨基酸突变位点主要集中在抗原表位。本试验结果提示该地区需制定科学的防控政策，防止病毒蔓延。

关键词: 猪圆环病毒3型(PCV3); 流行病学; 分子遗传进化

Abstract: 【Objective】 The aim of this study was to investigate and analyse the prevalence and molecular genetic evolution of Porcine circovirus type 3 (PCV3) in Chengdu area.【Method】 A total of 2 113 dead pig tissue samples were collected from 4 harmless disposal sites in Chengdu area from January 2023 to December 2023,and the PCV3 infection was detected by Real-time quantitative PCR.Three pairs of primers were designed for PCR amplification and sequencing of PCV3 genome sequence of positive samples.Through online software splicing PCV3 genome sequence,intercepting ORF2 gene sequence and deducing Cap amino acid sequence,similarity comparison was carried out and genetic evolution tree was constructed.The mutation of Cap amino acid sequence was analyzed.【Result】 The positive rate of PCV3 in 2 113 dead pig tissue samples was 27.21% (575/2 113).According to PCR amplification and sequencing results,the genome sequences of 15 PCV3 strains were obtained by splicing.The similarity analysis showed that the nucleotide sequences similarity among the 15 PCV3 genome was 97.2%-100%,the nucleotide sequences similarity among ORF2 genes was 97.2%-100%,and the amino acid sequences similarity among Cap sequences was 96.7%-100%.The results of genetic evolution tree showed that 12 PCV3 belonged to PCV3a subtype and 3 belonged to PCV3c subtype.A total of 13 of the 15 Cap amino acid sequences were mutated,and there were 13 mutation sites.【Conclusion】 PCV3 in Chengdu area was mainly PCV3a and PCV3c subtypes with high genomic similarity and conservative sequences.The mutation sites of Cap amino acids were mainly concentrated in antigen epitope.The results suggested that scientific prevention and control policies should be formulated in this region to prevent the spread of the virus.

Key words: Porcine circovirus type 3 (PCV3); epidemiology; molecular genetic evolution

中图分类号:

S852.65⁺9.2

黄云川, 范磊, 林鑫, 雷玉, 王兴丽, 徐志文, 岳建国. 2023年成都地区猪圆环病毒3型流行病学调查及遗传进化分析[J]. 中国畜牧兽医, 2024, 51(10): 4531-4539.

HUANG Yunchuan, FAN Lei, LIN Xin, LEI Yu, WANG Xingli, XU Zhiwen, YUE Jianguo. Epidemiological Investigation and Genetic Evolution Analysis of Porcine Circovirus Type 3 of Chengdu in 2023[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(10): 4531-4539.

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