葡萄渣对肉母牛外周血淋巴细胞亚群、淋巴细胞转化率与凋亡率及抗氧化性的影响

doi:10.16431/j.cnki.1671-7236.2024.04.021

摘要/Abstract

摘要： 【目的】研究葡萄渣对肉母牛外周血淋巴细胞亚群、淋巴细胞转化率与凋亡率及抗氧化性的影响。【方法】选取胎次、体重相近的产后健康肉母牛20头,随机分为4组,每组5头。对照组饲喂基础饲粮,试验Ⅰ、Ⅱ、Ⅲ组每头在基础饲粮中分别添加240、300、360 g/d的葡萄渣。试验预试期15 d,正试期40 d。采集尾静脉血,测定其外周血淋巴细胞亚群、淋巴细胞的转化率与凋亡率及其抗氧化水平。【结果】①试验第20天,3个试验组肉母牛外周血CD4⁺T细胞比例较对照组均显著上升(P＜0.05);试验第40天,试验Ⅱ、Ⅲ组CD4⁺T细胞比例较对照组显著升高(P＜0.05),CD8⁺T细胞比例较对照组显著降低(P＜0.05),3个试验组CD4⁺/CD8⁺比值较对照组均显著升高(P＜0.05)。②试验第20、40天,试验Ⅱ、Ⅲ组母牛外周血淋巴细胞转化率均极显著高于对照组(P＜0.01)。试验第40天,随着葡萄渣添加量升高,外周血淋巴细胞凋亡率呈线性下降(P＜0.01)。③试验第40天,3个试验组肉母牛血清MDA含量较对照组均显著降低(P＜0.05),试验Ⅰ、Ⅲ组肉母牛血清T-AOC含量均极显著高于对照组(P＜0.01)。【结论】肉母牛饲粮添加不同比例葡萄渣可以改善肉牛的免疫功能,本试验条件下,葡萄渣的适宜添加水平为(300~360) g/d。

关键词: 肉母牛; 葡萄渣; 淋巴细胞; 抗氧化性

Abstract: 【Objective】 The aim of this study was to study the effects of grape pomace on lymphocyte subsets,lymphocyte conversion rate,apoptosis rate and antioxidant activity in peripheral blood of cows.【Method】 Twenty healthy postpartum beef cows with similar parity and body weight were randomly divided into 4 groups with 5 cows in each group.The beef cows in control group were fed a basal diet,and 240,300 and 360 g/d grape dregs were added to the basal diet per head in trial groups I,Ⅱ and Ⅲ,respectively.The pre-trial period lasted for 15 days,and the experimental period lasted for 40 days.The tail vein blood was collected and the lymphocyte subsets,the conversion rate and apoptosis rate of lymphocytes and the antioxidant level of peripheral blood were measured.【Result】 ①On the 20th day of the experiment,the proportion of CD4⁺T cells in peripheral blood of beef cows in all three experimental groups were significantly increased compared to the control group (P＜0.05).On the 40th day of the experiment,the proportion of CD4⁺T cells in groups Ⅱ and Ⅲ were significantly increased compared to the control group (P＜0.05),while the proportion of CD8⁺T cells were significantly reduced compared to the control group (P＜0.05).The CD4⁺/CD8⁺ ratio in all three experimental groups were significantly increased compared to the control group (P＜0.05).②On the 20th and 40th day of the experiment,the peripheral blood lymphocyte transformation rates of cows in groups Ⅱ and Ⅲ were significantly higher than those in the control group (P＜0.01).On the 40th day of the experiment,as the amount of grape residue added increased,the apoptosis rate of peripheral blood lymphocytes showed a linear and extremely significant decrease (P＜0.01).③On the 40th day of the experiment,the serum MDA content of three experimental groups of beef cows were significantly reduced compared to the control group (P＜0.05).The serum T-AOC content of beef cows in experimental groups Ⅰ and Ⅲ were significantly higher than that of the control group (P＜0.01).【Conclusion】 Adding different proportions of grape pomace to the diet of beef cows could improve their immune function.Under the experimental conditions,the appropriate level of grape pomace addition was (300-360) g/d.

Key words: beef cow; grape pomaces; lymphocyte; antioxidant activity

中图分类号:

S823.9

王建东, 丁辉, 刘欣, 侯鹏霞, 梁小军, 高海慧, 王瑞刚, 姜武. 葡萄渣对肉母牛外周血淋巴细胞亚群、淋巴细胞转化率与凋亡率及抗氧化性的影响[J]. 中国畜牧兽医, 2024, 51(4): 1530-1536.

WANG Jiandong, DING Hui, LIU Xin, HOU Pengxia, LIANG Xiaojun, GAO Haihui, WANG Ruigang, JIANG Wu. Effects of Grape Pomace on Lymphocyte Subsets,Lymphocyte Transformation Rate,Apoptosis Rate and Antioxidant Activity in Peripheral Blood of Beef Cows[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(4): 1530-1536.

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