柔嫩艾美耳球虫对藏鸡和隐性白羽鸡致病性差异分析

doi:10.16431/j.cnki.1671-7236.2019.10.028

摘要/Abstract

摘要： 为进一步明确柔嫩艾美耳球虫对球虫易感性差异鸡种的致病性，本研究以1×10⁵个柔嫩艾美耳球虫孢子化卵囊的剂量分别感染对球虫具有抗性的藏鸡和易感的隐性白羽鸡，接种后观察记录各组鸡的临床表征、血便记分、死亡率、增重、盲肠病变记分、卵囊产量，并于感染前和感染后3、6和8 d每个品种分别随机选择5只鸡采集抗凝血，应用流式细胞仪（FCAS）检测外周血CD4⁺T和CD8⁺T淋巴细胞亚群数量。结果显示，柔嫩艾美耳球虫感染后藏鸡相对增重率高于隐性白羽鸡，死亡率、血便记分和盲肠病变记分均低于隐性白羽鸡，但卵囊产量高于隐性白羽鸡。外周血T淋巴细胞亚群变化方面，感染前，藏鸡CD4⁺T淋巴细胞数及CD4⁺/CD8⁺比值均高于隐性白羽鸡。感染后第3天，藏鸡CD4⁺、CD8⁺ T淋巴细胞数及CD4⁺/CD8⁺比值下降，隐性白羽鸡CD8⁺ T淋巴细胞数略有下降，CD4⁺T淋巴细胞数及CD4⁺/CD8⁺比值上升，但CD4⁺/CD8⁺比值仍显著低于藏鸡（P<0.05）。感染后第6天，2个品种鸡CD4⁺ T淋巴细胞数及CD4⁺/CD8⁺比值均下降，其中藏鸡表现为显著下降（P<0.05），而隐性白羽鸡仅CD4⁺/CD8⁺比值显著降低（P<0.05），隐性白羽鸡CD8⁺ T淋巴细胞数显著升高（P<0.05）。感染后第8天，2个品种鸡CD4⁺/CD8⁺比值显著下降（P<0.05），藏鸡CD8⁺ T淋巴细胞数显著高于隐性白羽鸡（P<0.05），但CD4⁺/CD8⁺比值显著低于隐性白羽鸡（P<0.05）。结果表明，球虫对藏鸡和隐性白羽鸡的致病性存在差异，这种差异与T淋巴细胞介导的免疫应答反应密切相关。

关键词: 藏鸡; 隐性白羽鸡; 柔嫩艾美耳球虫; 致病性; T淋巴细胞亚群

Abstract: In order to further clarify the pathogenicity of Eimeria tenella in chickens with different susceptibility to coccidia,we infected Tibetan chickens and Recessive White chickens with resistance to coccidia at the dosage of 1×10⁵ sporulated oocysts of Eimeria tenella.After inoculation,the clinical characteristics,blood stool score,mortality and susceptibility to coccidia were observed and recorded.Weight gain,cecum lesion score and oocyst yield were recorded.Five chickens were randomly selected from each breed before infection and 3,6 and 8 days after infection to collect anticoagulant.The number of CD4⁺,CD8⁺ T lymphocyte subsets in peripheral blood was detected by flow cytometry (FCAS).The results showed that the relative weight gain rate of Tibetan chickens infected with Eimeria tenella was higher than that of Recessive White chickens.The mortality rate,blood stool score and cecum lesion score of Tibetan chickens were lower than those of Recessive White chickens,but the oocyst yield was higher than that of Recessive White chickens.Before infection,the number of CD4⁺ T lymphocyte and the ratio of CD4⁺/CD8⁺ in Tibetan chickens were higher than those in Recessive White chickens.On the 3rd day after infection,the number of CD4⁺,CD8⁺ T lymphocyte and CD4⁺/CD8⁺ ratio in Tibetan chickens decreased,while the number of CD8⁺ T lymphocyte in Recessive White chickens decreased slightly,the number of CD4⁺ T lymphocyte and the ratio of CD4⁺/CD8⁺ increased,but the ratio of CD4⁺/CD8⁺ was still significantly lower than that in Tibetan chickens (P<0.05).On the 6th day after infection,the number of CD4⁺ T lymphocyte and the ratio of CD4⁺/CD8⁺ decreased in both breeds,Tibetan chickens showed a significant decrease (P<0.05),while only the ratio of CD4⁺/CD8⁺ in Recessive White chickens significantly decreased (P<0.05),the number of CD8⁺ T lymphocyte in Recessine White chickens significantly increased (P<0.05).On the 8th day after infection,the ratio of CD4⁺/CD8⁺ in two breeds of chickens significantly decreased (P<0.05).The number of CD8⁺ T lymphocyte in Tibetan chickens was significantly higher than that in Recessive White chickens (P<0.05),but the ratio of CD4⁺/CD8⁺ was significantly lower than that in Recessive White chickens (P<0.05).The results indicated that the pathogenicity of coccidia to Tibetan chickens and Recessive White chickens was different,which was closely related to the immune response mediated by T lymphocyte.

Key words: Tibetan chickens; Recessive White chickens; Eimeria tenella; pathogenicity; T lymphocyte subpopulations

中图分类号:

S852.72

李建梅, 刘梅, 沈欣悦, 戴亚斌, 俞燕. 柔嫩艾美耳球虫对藏鸡和隐性白羽鸡致病性差异分析[J]. 中国畜牧兽医, 2019, 46(10): 3058-3064.

LI Jianmei, LIU Mei, SHEN Xinyue, DAI Yabin, YU Yan. Analysis of Pathogenicity Difference Between Tibetan Chickens and Recessive White Chickens Following Eimeria tenella Infection[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(10): 3058-3064.

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