Ⅰ群禽腺病毒血清11型的分离鉴定及致病性试验

doi:10.16431/j.cnki.1671-7236.2022.03.029

摘要/Abstract

摘要： 【目的】了解江苏地区禽腺病毒（Fowl adenovirus，FAdV）的基因遗传演化情况及致病性，为FAdV的流行病学研究和疫病防控提供参考。【方法】通过SPF鸡胚盲传、PCR鉴定、电镜观察、全基因组测序、序列比对与相似性分析判定病毒血清分型，并将分离株以5×10^5.33 EID₅₀剂量胸肌注射的方式感染10日龄SPF雏鸡进行动物回归试验。【结果】 PCR结果显示，该分离株为Ⅰ群FAdV阳性，在透射电镜下可见球型、无囊膜、具有腺病毒典型的二十面体结构，通过全基因组和Hexon基因进行序列分析表明该分离毒株为FAdV D种血清11型毒株，命名为JSNT-1株。将该毒株感染SPF雏鸡，死亡率为10%（1/10），病死鸡剖检可见肝脏褪色变黄，出血肿胀，边缘钝圆；肾脏肿大出血，苍白等病变。通过实时荧光定量PCR试验可从口腔及泄殖腔中检测到排毒，且病毒在鸡体内多个组织器官均有分布。病理组织学结果显示，死亡鸡的肝细胞变性坏死，出现嗜碱性核内包涵体；肾小球上皮细胞变性坏死；心肌可见大量炎性细胞浸润。【结论】分离株为FAdV血清11型，感染SPF鸡后临床发病不明显，致死率低，病死鸡能产生特征性的包涵体肝炎病变，且病毒可在鸡体内外进行复制。

关键词: 禽腺病毒血清11型; 包涵体肝炎; 分离鉴定; 遗传进化分析; 致病性

Abstract: 【Objective】 This study was aimed to understand the genetic evolution and pathogenicity of Fowl adenovirus (FAdV) in Jiangsu area, and provide reference for the epidemiological study of FAdV and disease prevention and control.【Method】 Serological typing of the virus was determined by chick embryo passage culture, PCR identification, electron microscope observation, whole genome sequencing, sequence alignment and similarity analysis, and the isolated strain were injected into the chest muscle with a dose of 5×10^5.33 EID₅₀ to infect 10-day-old SPF chicks for animal regression experiments.【Result】 PCR results showed that the isolated strain was positive for group Ⅰ FAdV, and the spherical, membraneless, icosahedral structure of adenovirus was observed under transmission electron microscope.The whole genome and Hexon gene sequence analysis showed that the isolated strain was serotype 11 of FAdV D, named JSNT-1 strain.The mortality rate of SPF chicks infected with this strain was 10%(1/10).Autopsy of the dead chickens showed that the liver was discolored and yellow, bleeding and swelling, and the edge of liver was blunt and round, kidney was swollen, bleeding and pale.Detoxification could be detected in oral cavity and cloaca by Real-time PCR test, and the virus was distributed in many tissues and organs in the chicken body.The histopathological results showed that the liver cells of the dead chickens were degenerated and necrotic, with basophilic intranuclear inclusion bodies, glomerular epithelial cells were degenerated and necrotic.A large number of inflammatory cell infiltrations were seen in the myocardium.【Conclusion】 The isolated strain was serotype 11 of FAdV, and the clinical incidence was not obvious after infection with SPF chickens, and the fatality rate was low.The sick and dead chickens could produce characteristic inclusion body hepatitis lesions, and the virus could replicate in and outside the chickens.

Key words: FAdV-11; inclusion body hepatitis; isolation and identification; genetic evolution analysis; pathogenicity

中图分类号:

S852.65

兰虹, 任广彩, 叶俊贤, 熊挺, 何献铭, 刘郁夫, 杨泽坤, 郝文茜, 陈瑞爱. Ⅰ群禽腺病毒血清11型的分离鉴定及致病性试验[J]. 中国畜牧兽医, 2022, 49(3): 1067-1076.

LAN Hong, REN Guangcai, YE Junxian, XIONG Ting, HE Xianming, LIU Yufu, YANG Zekun, HAO Wenqian, CHEN Ruiai. Isolation, Identification and Pathogenicity of Fowl Adenovirus Group Ⅰ Serotype 11[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1067-1076.

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