罗伊氏乳酸杆菌LR1对猪蛋白消化酶基因表达、肌肉抗氧化指标及氨基酸组成的影响

doi:10.16431/j.cnki.1671-7236.2021.06.017

中国畜牧兽医 ›› 2021, Vol. 48 ›› Issue (6): 2045-2055.doi: 10.16431/j.cnki.1671-7236.2021.06.017

罗伊氏乳酸杆菌LR1对猪蛋白消化酶基因表达、肌肉抗氧化指标及氨基酸组成的影响

田志梅¹, 鲁慧杰¹, 邓盾¹, 崔艺燕¹, 刘志昌¹, 李家洲¹, 马现永^1,2

1. 广东省农业科学院动物科学研究所, 农业农村部华南动物营养与饲料重点实验室, 畜禽育种国家重点实验室, 广东省畜禽育种与营养研究重点实验室, 广东省畜禽肉品质量安全控制与评定工程技术研究中心, 广州 510640;
2. 岭南现代农业科学与技术广东省实验室茂名分中心, 茂名 525000

收稿日期:2020-10-29 出版日期:2021-06-20 发布日期:2021-06-18
通讯作者: 马现永 E-mail:407986619@qq.com
作者简介:田志梅(1985-),女,黑龙江哈尔滨人,硕士,研究方向:动物营养与饲料科学、生态养殖与环境控制,E-mail:382874346@qq.com
基金资助:
广东省基础与应用基础研究基金（2020A151010591、2019B1515210032、2018A0303130004）；广东省农业科学院人才项目（201803、R2017YJ-YB3007、R2018QD-071）；清远市创新创业科研团队项目（2018002）。

Effect of Lactobacillus reuteri 1 on the Gene Expression of Protein Digestion-related Enzymes,Antioxidant Indexes and Amino Acids Composition of Muscle in Pigs

TIAN Zhimei¹, LU Huijie¹, DENG Dun¹, CUI Yiyan¹, LIU Zhichang¹, LI Jiazhou¹, MA Xianyong^1,2

1. Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, State Key Laboratory of Livestock and Poultry Breeding, Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Guangdong Engineering Technology Research Center of Animal Meat Quality and Safety Control and Evaluation, Guangzhou 510640, China;
2. Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Maoming 525000, China

Received:2020-10-29 Online:2021-06-20 Published:2021-06-18

摘要/Abstract

摘要： 试验旨在研究长期饲喂罗伊氏乳酸杆菌LR1（Lactobacillus reuteri 1）对猪胃及胰腺蛋白消化相关酶的基因表达、肌肉抗氧化指标及冰鲜肉氨基酸谱的影响。选用144头（杜×长×大）21日龄断奶仔猪（平均体重6.49 kg±0.04 kg），随机分为3组。对照组饲粮为基础饲粮，抗生素组饲粮为添加抗生素的基础饲粮（仔猪阶段添加100 mg/kg喹乙醇和75 mg/kg金霉素，生长肥育猪阶段添加75 mg/kg金霉素），LR1组饲粮为添加5×10¹⁰ CFU/kg LR1的基础饲粮，试验期175 d。结果表明：与对照组相比，①抗生素组猪胰腺中弹性蛋白酶的表达降低（P<0.05）；LR1组胃窦、胃贲门及胃底黏膜中胃蛋白酶A的表达提高（P<0.05），同时也高于抗生素组（P<0.05）。②抗生素组猪背最长肌（longissimus dorsis muscle，LDM）总抗氧化能力（total antioxidant capacity，T-AOC）及总超氧化物歧化酶（total superoxide dismutase，T-SOD）活力增加（P<0.05）；LR1不影响LDM中丙二醛（malondialdehyde，MDA）含量，T-AOC、T-SOD及谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）活力（P>0.05）。③饲粮添加抗生素及LR1不影响冰鲜肉水解氨基酸含量及组成（P>0.05）；抗生素组24 h冰鲜肉中甲硫氨酸（苦味氨基酸）含量、甜味氨基酸总量及游离氨基酸总量降低（P<0.05），而LR1降低24 h冰鲜肉中甲硫氨酸含量（P<0.05）；饲粮中添加抗生素及LR1均降低48 h冰鲜肉中甜味氨基酸、苦味氨基酸、必需氨基酸及总游离氨基酸（P<0.05），同时，抗生素组48 h冰鲜肉中鲜味氨基酸含量降低（P<0.05）。综上，与对照组相比，长期饲喂LR1可提高猪胃对蛋白质的消化功能，改善24 h冰鲜肉的风味；而长期饲喂抗生素降低猪胰腺的消化功能，提高肌肉抗氧化能力，但降低24及48 h冰鲜肉的风味。

关键词: 罗伊氏乳酸杆菌; 猪; 消化酶; 抗氧化性能; 氨基酸

Abstract: This experiment was conducted to study effects of long-term Lactobacillus reuteri 1 (LR1) on the expression of enzymes involved in protein digestion in pancreas and stomach,antioxidant capacity and amino acid profile of meat in pigs.A total of 144 cross-bred (Duroc×Landrace×Yorkshire) weaned piglets (average body weight 6.49 kg±0.04 kg) at 21 days were randomly assigned to 3 groups.The diet in control groups was basal diet,the diet in antibiotic group was basal diet supplemented with antibiotics (100 mg/kg olaquindox plus 75 mg/kg aureomycin for weaned piglets,and 75 mg/kg aureomycin for growing and finishing pigs),and the diet in LR1 group was basal diet supplemented with 5×10¹⁰ CFU/kg LR1.The experiment lasted for 175 days.The results showed as follows:① Antibiotics decreased the expression of elastase in pancreas compared to control (P<0.05),LR1 increased pepsinogen A in antrum,cardia and fundus of stomach in pigs compared to control and antibiotics (P<0.05).② Compared to control group,antibiotics increased activities of total axtioxidant capacity (T-AOC) and total superoxide dismutase (T-SOD) in longissimus dorsis muscle (LDM) (P<0.05).LR1 did not affect malondialdehyde (MDA) content,activities of T-AOC、SOD and glutathione peroxidase (GSH-Px)in LDM of pigs (P>0.05).③ Both antibiotics and LR1 did not affect significantly total amino acids profile following hydrolysis (P>0.05).Antibiotics decreased free methionine (bitter amino acids),total content of sweet amino acids and free amino acids compared to chilled fresh pork at 24 h in contron group (P<0.05),and LR1 decreased methionine (P<0.05).Antibiotics and LR1 decreased the content of sweet,bitter,essential amino acids and total free amino acids (P<0.05),additionally,antibiotics decreased the content of flavor amino acids of chilled fresh pork at 48 h (P<0.05).In conclusion,long-term LR1 improved digestive function of protein in stomach and flavor of chilled fresh pork at 24 h of pigs.Long-term antibiotics decreased digestive function of pancreas,improved antioxidant capacity of muscle,but decreased flavor of chilled fresh pork at 24 h and 48 h of pigs.

Key words: Lactobacillus reuteri; pigs; digestive enzymes; antioxidant capacity; amino acids

中图分类号:

S816.7

田志梅, 鲁慧杰, 邓盾, 崔艺燕, 刘志昌, 李家洲, 马现永. 罗伊氏乳酸杆菌LR1对猪蛋白消化酶基因表达、肌肉抗氧化指标及氨基酸组成的影响[J]. 中国畜牧兽医, 2021, 48(6): 2045-2055.

TIAN Zhimei, LU Huijie, DENG Dun, CUI Yiyan, LIU Zhichang, LI Jiazhou, MA Xianyong. Effect of Lactobacillus reuteri 1 on the Gene Expression of Protein Digestion-related Enzymes,Antioxidant Indexes and Amino Acids Composition of Muscle in Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2045-2055.

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罗伊氏乳酸杆菌LR1对猪蛋白消化酶基因表达、肌肉抗氧化指标及氨基酸组成的影响

Effect of Lactobacillus reuteri 1 on the Gene Expression of Protein Digestion-related Enzymes,Antioxidant Indexes and Amino Acids Composition of Muscle in Pigs

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