干酪乳杆菌YG-01株的益生特性及抑菌活性研究

doi:10.16431/j.cnki.1671-7236.2024.02.039

摘要/Abstract

摘要： 【目的】评价从奶酪中分离出的干酪乳杆菌YG-01的益生性及抑菌活性,为该菌株在食品动物生产中的应用提供理论参考。【方法】本研究分别检测干酪乳杆菌YG-01株的药物敏感性、肠道定植能力、消化道环境耐受特性、肠道菌群调节作用、抑制肠道致病菌活性及促生长作用。【结果】药敏试验结果显示,干酪乳杆菌YG-01对米诺环素、红霉素、克拉霉素、克林霉素、青霉素等常见抗生素极敏感;利用探针(carboxyfluorescein diacetate,succinimidyl ester,cFDA-SE)标记的干酪乳杆菌YG-01灌喂试验兔,口服后第1天,干酪乳杆菌YG-01在兔空肠、回肠和结肠的检出率分别为70.9%、59.3%和66.0%,口服后第11天时仍保持较高定植水平(＞35%);干酪乳杆菌YG-01的活菌数在模拟胃液(pH为2.5、3.5、4.5)、模拟肠液、胆酸盐浓度＜2 g/L及在9% NaCl溶液中均高于1×10⁵ CFU/mL;体外抑菌试验结果显示,干酪乳杆菌YG-01能显著抑制大肠埃希菌O157、小肠结肠炎耶尔森菌、沙门菌、志贺菌、副溶血弧菌等肠道致病菌生长,而对益生菌罗伊乳杆菌无抑制作用;口服干酪乳杆菌YG-01能极显著提高兔的平均日采食量和平均日增重,还可显著抑制厚壁菌门和疣微菌门细菌增殖。【结论】干酪乳杆菌YG-01株具有良好的益生性,可显著抑制肠道致病菌的增殖,改善食品动物生长性能,为开发基于干酪乳杆菌YG-01株的微生态制剂奠定了基础。

关键词: 干酪乳杆菌YG-01株; 益生特性; 抑菌活性; 微生态制剂

Abstract: 【Objective】 The present study was aimed to evaluate the probiotic properties and inhibitory activity against pathogenic bacteria of Lactobacillus casei (L. casei) YG-01 isolated from cheese, and provide a theoretical reference for the application of this strain in food animal production practices.【Method】 This study evaluated the drug sensitivity, intestinal colonization ability, digestive tract environmental tolerance, modulation of intestinal microbiota, inhibition of pathogenic bacteria activity, and growth promotion effect of the L. casei YG-01 strain.【Result】 The drug sensitivity test showed that L. casei YG-01 was highly sensitive to common antibiotics such as minocycline, erythromycin, clarithromycin, clindamycin, and penicillin.Using carboxyfluorescein diacetate, succinimidyl ester (cFDA-SE) labeled L. casei YG-01 for oral administration in rabbits, the detection rates of L. casei YG-01 in rabbit jejunum, ileum and colon on the first day were 70.9%, 59.3% and 66.0%, respectively, and the high colonization levels (＞35%) were maintained on the 11th day after oral administration.The viable numbers of L. casei YG-01 were higher than 1×10⁵ CFU/mL in simulated gastric juice at pH 2.5, 3.5 and 4.5, simulated intestinal juice, 2 g/L cholate concentration and 9% NaCl solution. In vitro antibacterial tests showed that L. casei YG-01 significantly inhibited the growth of pathogenic bacteria in the intestines, such as Escherichia coli O157, Yersinia enterocolitica, Salmonella, Shigella, and Vibrio parahaemolyticus, while having no inhibitory effect on the probiotic bacterium Lactobacillus rhamnosus.Oral administration of L. casei YG-01 significantly increased average daily feed intake and average daily gain, but inhibited the proliferation of bacteria from the phyla Firmicutes and Proteobacteria.【Conclusion】 L. casei YG-01 exhibited excellent probiotic properties, as it could significantly inhibit the proliferation of pathogenic bacteria in the intestines and improve food animal growth performance.These findings laid a foundation for the development of microbiota-based preparations using the L. casei YG-01 strain.

Key words: Lactobacillus casei YG-01; probiotic characteristics; antibacterial activity; microecological preparations

中图分类号:

刘伊湄, 周佳莹, 韩言言, 李园, 宋厚辉, 徐义刚, 王美. 干酪乳杆菌YG-01株的益生特性及抑菌活性研究[J]. 中国畜牧兽医, 2024, 51(2): 829-836.

LIU Yimei, ZHOU Jiaying, HAN Yanyan, LI Yuan, SONG Houhui, XU Yigang, WANG Mei. Probiotic Characteristics and Antibacterial Activity of Lactobacillus casei Strain YG-01[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(2): 829-836.

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