牛至油博落回口服液的研制及体外抑菌活性测定

doi:10.16431/j.cnki.1671-7236.2022.02.034

摘要/Abstract

摘要： 【目的】研制牛至油博落回口服液, 并测定其体外抑菌活性及其主要成分的联合抑菌效果, 为临床用药提供理论依据。【方法】通过预试验和Box-Behnken响应面法优化处方; 采用高效液相色谱法测定口服液主要成分含量; 采用滤纸片法测定口服液对大肠杆菌、沙门氏菌、金黄色葡萄球菌和粪链球菌的抑菌圈直径; 采用试管二倍稀释法测定口服液、5%牛至油溶液及1%博落回溶液对4种细菌的最小抑菌浓度(MIC)和最小杀菌浓度(MBC); 采用微量棋盘稀释法对5%牛至油溶液与1%博落回溶液进行体外联合药敏试验。【结果】牛至油博落回口服液最优配方为: 5%牛至油, 1%博落回提取物, 25%增溶剂聚氧乙烯(40)氢化蓖麻油, 0.02%抗氧化剂2, 6-二叔丁基对甲酚(BHT), 余量为水。口服液中香芹酚的含量为42.59 mg/mL, 血根碱含量为6.51 mg/mL。口服液对4种菌的抑菌圈直径分别为16.9、16.4、23.7和17.0 mm, MIC分别为3.125、3.125、1.5625和1.5625 μL/mL, MBC分别为12.5、6.25、3.125和3.125 μL/mL; 5%牛至油溶液对4种菌的MIC分别为25、12.5、6.25和100 μL/mL, MBC分别为100、50、25和>200 μL/mL; 1%博落回溶液对4种菌的MIC分别为6.25、12.5、3.125和6.25 μL/mL, MBC分别为50、25、6.25和25 μL/mL。联合药敏试验表明, 二者联合用药对大肠杆菌、沙门氏菌起相加作用, 对金黄色葡萄球菌无作用, 对粪链球菌为协同作用。【结论】试验制备了牛至油博落回口服溶液剂, 该制剂对大肠杆菌、沙门氏菌、金黄色葡萄球菌和粪链球菌具有良好抑制作用。

关键词: 牛至油; 博落回; 响应面法; 抑菌活性;

Abstract: 【Objective】 This study was aimed to prepare oregano oil Macleaya cordata oral solution, determine its antibacterial activity in vitro and provide theoretical reference for clinical use of new veterinary drugs.【Method】 The formulae were optimized by pre-test and Box-Behnken response surface method.The main components of oral solutiom were determined by high performance liquid chromatography (HPLC).The inhibition zone diameters of oral solutiona against Escherichia coli, Salmonella, Staphylococcus aureus and Streptococcus faecalis were determined by disk diffusion method.The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of oral solution, 5% oregano oil solution and 1% Macleaya cordata solution against 4 kinds of bacteria were determined by tube double dilution method.The combined susceptibility test in vitro of 5% oregano oil solution and 1% Macleaya cordata solution was carried out with checkerboard microdilution test.【Result】 The optimal formula of oregano oil Macleaya cordata oral solution was 5% oregano oil, 1% Macleaya cordata, 25% solubilizer RH40, 0.02% antioxidant BHT and water.The concentration of carvacrol and sanguinarine was 42.59 and 6.51 mg/mL in oral solution, respectively.The inhibition zone diameters of oral solution to 4 bacteria were 16.9, 16.4, 23.7 and 17.0 mm, MIC were 3.125, 3.125, 1.5625 and 1.5625 μL/mL, and MBC were 12.5, 6.25, 3.125 and 3.125 μL/mL, respectively; The MIC of 5% oregano oil solution to 4 bacteria were 25, 12.5, 6.25 and 100 μL/mL, and MBC were 100, 50, 25 and > 200 μL/mL, respectively.The MIC of 1% Macleaya cordata solution to 4 bacteria were 6.25, 12.5, 3.125 and 6.25 μL/mL, and MBC were 50, 25, 6.25 and 25 μL/mL, respectively.The combined susceptibility test showed that the two combined components had additive effect on Escherichia coli and Salmonella, irrelevant effect on Staphylococcus aureus, and synergistic effect on Streptococcus faecalis.【Conclusion】 Oregano oil Macleaya cordata oral solution was prepared in this study, it had good inhibitory effects on Escherichia coli, Salmonella, Staphylococcus aureus and Streptococcus faecalis.

Key words: oregano oil; Macleaya cordata; response surface method; antibacterial activity

中图分类号:

S852.61

李伟浩, 孙媛媛, 刘聚祥. 牛至油博落回口服液的研制及体外抑菌活性测定[J]. 中国畜牧兽医, 2022, 49(2): 718-730.

LI Weihao, SUN Yuanyuan, LIU Juxiang. Preparation of Oregano Oil Macleaya cordata Oral Solution and Its Antibacterial Activity in vitro[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 718-730.

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