抗菌肽BSN-37的抑菌活性及其稳定性分析

doi:10.16431/j.cnki.1671-7236.2019.01.034

摘要/Abstract

摘要：

试验旨在研究抗菌肽BSN-37的抑菌活性和稳定性。采用微量倍比稀释法测定抗菌肽BSN-37的最小抑菌浓度（MIC），菌落计数法分析抗菌肽BSN-37对大肠杆菌CVCC1568的杀菌动力学特征，扩散法测定抗菌肽BSN-37的盐离子稳定性、酸碱稳定性、血清稳定性、胰酶稳定性、热稳定性、反复冻融稳定性、室温保持稳定性、药效保持时间稳定性。结果显示，抗菌肽BSN-37对革兰氏阴性菌（大肠杆菌和沙门氏菌）的MIC为1.5625~25 μg/mL，对革兰氏阳性菌（枯草芽孢杆菌和短小芽孢杆菌）的MIC为1.5625~12.5 μg/mL，对真菌（白色念珠菌）没有活性；抗菌肽BSN-37可在90 min内完全杀灭大肠杆菌CVCC1568；除了Fe²⁺和胰酶会影响抗菌肽BSN-37的抑菌活性外，在75~100 ℃的高温、150~250 mmol/L高浓度盐离子（Na⁺、K⁺、Ca²⁺和Mg²⁺）、20%~25%饱和浓度的Cu²⁺、pH 4~10、20%~25%的血清、10~12次的反复冻融、10~12 d室温保存等条件下仍能保持较好的抑菌活性，药效保持时间可达7.5 d。本试验结果表明，抗菌肽BSN-37具有替代抗生素成为新抗菌药物的潜力，为抗菌肽BSN-37的临床应用提供了理论依据。

关键词: 抗菌肽BNS-37; 抑菌活性; 稳定性; 理化特性; 杀菌动力学

Abstract:

The aim of the experiment was to study the bacteriostatic activity and stability of the antimicrobial peptide BSN-37.The minimum inhibitory concentration (MIC) of BSN-37 against several bacteria were detected with serial dilution method.The bactericidal dynamics of BSN-37 against Escherichia coli (E.coli) CVCC1568 were measured by colony counting method.The salt ion stability,acid-alkali stability,serum stability,pancreatic enzyme stability,thermal stability,repeated freeze-thaw stability,maintain stability at room temperature,and efficacy stabilization stability were analyzed through diffusion method.The results showed that the MIC of the antimicrobial peptide BSN-37 against Gram negative bacteria (E.coli and Salmonella) was 1.5625 to 25 μg/mL,and the MIC of BSN-37 against Gram positive bacteria (Bacillus subtilis and Bacillus pumilus) were 1.5625 to 12.5 μg/mL.But BSN-37 had no bacteriostatic activity against fungi (Candida albicans).The antimicrobial peptide BSN-37 could completely kill E.coli CVCC1568 within 90 min.In addition to the Fe²⁺ and pancreatic enzymes could affect the bacteriostatic activity of antimicrobial peptide BSN-37,at other conditions including the high temperature (75 to 100℃),150 to 250 mmol/L high concentration of salt ions (Na⁺,K⁺,Ca²⁺ and Mg²⁺) and 20% to 25% of saturation concentration of Cu²⁺,pH 4 to 10,20% to 25% of serum,10 to 12 times of repeated freezing and thawing,preservation for 10 to 12 days at room temperature,BSN-37 could still maintain good bacteriostatic activity.Efficacy maintain time of BSN-37 was more than 7.5 days.The results provided a theoretical basis for the clinical application of antimicrobial peptide BSN-37 which had a potential to replace antibiotics as new antimicrobial agents.

Key words: antibacterial peptide BSN-37; bacteriostatic activity; stability; physical and chemical properties; bactericidal dynamics

中图分类号:

S859.79⁺7

张炜, 杭柏林, 司素锦, 宁春妹, 李杰, 胡建和. 抗菌肽BSN-37的抑菌活性及其稳定性分析[J]. 《中国畜牧兽医》, 2019, 46(1): 287-295.

ZHANG Wei, HANG Bolin, SI Sujin, NING Chunmei, LI Jie, HU Jianhe. Bacteriostatic Activity and Stability Analysis of Antimicrobial Peptide BSN-37[J]. , 2019, 46(1): 287-295.

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