一株解淀粉芽孢杆菌的益生特性及抑菌性研究

doi:10.16431/j.cnki.1671-7236.2020.02.013

摘要/Abstract

摘要： 本试验旨在研究自筛解淀粉芽孢杆菌DHN04的生长曲线、人工胃肠液、pH及猪胆盐耐受性，并研究其抑菌特性及抗生素敏感性。试验采用生长速率法测定该菌生长曲线，活菌计数法测定人工胃肠液耐受性、耐酸性和耐胆盐等特性，牛津杯法测定抑菌活性，药敏试片测定抗生素敏感性。结果表明，解淀粉芽孢杆菌DHN04在经过4~6 h的缓慢生长期后，进入对数生长期，12~24 h为该菌的稳定生长期；在人工胃液和人工肠液中保持3 h后存活率分别为55.55%和53.33%；pH 2.0时该菌存活率为14.14%，随着酸性的减弱，存活率逐渐上升，在pH 7.0时存活率可以达到93.85%；随着胆盐浓度增加，解淀粉芽孢杆菌的存活率逐渐下降，相同的胆盐浓度，24 h的存活率低于3 h。解淀粉芽孢杆菌对大肠杆菌、沙门氏菌、金黄色葡萄球菌及四联球菌均具有一定的抑制作用；对抗生素阿莫西林耐药。综上，解淀粉芽孢杆菌能够快速活化，对人工胃肠液、胆盐及pH的耐受性良好，对大肠杆菌、沙门氏菌和金黄色葡萄球菌等具有一定的抑制作用，对阿莫西林耐药。因此，解淀粉芽孢杆菌DHN04可作为一种潜在的益生菌菌株应用于畜禽养殖生产。

关键词: 解淀粉芽孢杆菌; 益生特性; 抑菌活性; 抗生素敏感性

Abstract: This experiment mainly studied the growth curve,artificial gastroenteric fluid,pH and pig bile salt tolerance of Bacillus amyloliquefaciens DHN04,and its bacteriostatic characteristics and antibiotic sensitivity.The growth curve of the bacteria was measured by growth rate method,the tolerance,acid resistance and bile salt resistance of artificial gastrointestinal fluid were measured by viable count method,the antibacterial activity was measured by Oxford cup method,and the antibiotic sensitivity was measured by drug sensitive test piece.The results showed that Bacillus amyloliquefaciens DHN04 entered the logarithmic growth phase after a slow growth period of 4-6 h,and the stable growth period of the bacteria was 12-24 h.After 3 h in artificial gastric juice and artificial intestinal fluid,the survival rates were 55.55% and 53.33%,respectively.The survival rate of the bacteria was 14.14% at pH 2.0.With the decrease of acidity,the survival rate increased gradually,and the survival rate reached 93.85% at pH 7.0.With the increase of bile salt concentration,the survival rate of Bacillus amyloliquefaciens decreased gradually.The same concentration of bile salt,the survival rate of 24 h was lower than that of 3 h.Bacillus amyloliquefaciens DHN04 had a certain inhibitory effect on Escherichia coli,Salmonella,Staphylococcus aureus and Tetracocci.It was resistant to amoxicillin.In summary,the characteristics of the Bacillus amyloliquefaciens DHN04 were found to be well tolerated by artificial gastrointestinal,bile salts and pH,and had certain inhibitory effects on Escherichia coli,Salmonella and Staphylococcus aureus,and could be activated quickly,which could be used as a potential probiotic strain for livestock and poultry production.

Key words: Bacillus amyloliquefaciens; probiotic characteristics; antibacterial activity; antibiotic sensitivity

中图分类号:

S816

夏超笃, 湛穗璋, 艾琴, 刘小艳, 方炳虎. 一株解淀粉芽孢杆菌的益生特性及抑菌性研究[J]. 中国畜牧兽医, 2020, 47(2): 425-432.

XIA Chaodu, ZHAN Suizhang, AI Qin, LIU Xiaoyan, FANG Binghu. A Study on the Probiotic Characteristics and Antibacterial Activity of a Strain of Bacillus amyloliquefaciens[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(2): 425-432.

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