补喂鞣花酸对哺乳中后期纯血马马驹粪便菌群多样性的影响

doi:10.16431/j.cnki.1671-7236.2023.11.017

摘要/Abstract

摘要： 【目的】试验旨在研究补喂不同水平的鞣花酸对马驹粪便细菌多样性的影响，通过探究粪便菌群的变化，以期为哺乳马驹胃肠道菌群多样性和肠道健康等提供参考依据。【方法】选择出生日期相近、体重为（143.33±16.10）kg的3月龄纯血马马驹15匹，随机分为3组，每组5匹，分别为对照组、试验Ⅰ组、试验Ⅱ组。在相同的饲养条件下，对照组马驹不做任何处理，试验Ⅰ和Ⅱ组马驹每天分别补喂15和30 mg/kg BW鞣花酸，进行为期60 d的饲养试验。试验第60天晨饲前于直肠采集马驹粪便样品，测定粪便菌群多样性。【结果】OTUs结果显示，试验各组共有OTUs 789个，试验Ⅰ组特有的OTUs为182个；各试验组与对照组之间Alpha多样性差异不显著（P>0.05），但试验Ⅰ组Shannon、Chao1及ACE指数有提高的趋势；菌群丰度结果表明，各试验组与对照组前十的优势菌种类相同，以厚壁菌门、拟杆菌门、放线菌门和疣微菌门为主，且丰度占总数92.94%以上，各组之间门、科和属水平菌群丰度差异均不显著（P>0.05）；LefSe分析结果表明，试验Ⅰ组有差异菌9种，试验Ⅱ组有2种；预测前十的功能有全局与概述图谱，碳水化合物代谢和氨基酸代谢等，但差异不显著（P>0.05）。【结论】哺乳期纯血马马驹粪便中优势菌群为厚壁菌门、拟杆菌门、放线菌门和疣微菌门，补喂15 mg/kg BW鞣花酸能够提高粪便菌群多样性，增加粪便菌群的种类。

关键词: 鞣花酸; 哺乳期; 马驹; 菌群多样性

Abstract: 【Objective】 The experiment aims to investigate the effect of supplementing different levels of ellagic acid on the diversity of fecal bacteria in foals, and to explore the changes in fecal microbiota, in order to provide reference for the diversity of gastrointestinal microbiota and intestinal health in lactating foals.【Method】 Fifteen three month old pure blood ponies with similar birth dates and a weight of (143.33±16.10) kg were selected for the experiment.All the foals were randomly divide into 3 groups with 5 foals in each group, named control group, group Ⅰ, and group Ⅱ.Under the same feeding conditions, the ponies in control group did not undergo any treatment and the ponies in group Ⅰ and Ⅱ were fed with 15 and 30 mg/kg BW ellagic acid every day for 60 days.On the 60th day of the experiment, fecal samples of the foal were collected by rectal sampling method before feeding, and the diversity of fecal microbiota was measured.【Result】 The OTUs results showed there was a total of 789 OTUs in all groups, and 182 unique was in group Ⅰ.There was no significant difference in alpha diversity between the experimental groups and the control group (P>0.05), but group Ⅰ showed a trend of increasing Shannon, Chao1, and ACE indices.In terms of microbial abundance, the top 10 dominant bacterial species in each experimental group and control group were the same, with the main being Firmicutes, Bacteroidetes, Actinobacteria, and Verrucomycetes, and the abundance accounts for more than 92.94% of the total.There was no significant difference in the abundance of phyla, family, and genus level microbial communities among each group (P>0.05).LefSe analysis showed that there were 9 species of differential bacteria in group Ⅰ and 2 species in group Ⅱ.The top 10 predicted functions include global and overview maps, carbohydrate metabolism, and amino acid metabolism, but the differences were not significant (P>0.05).【Conclusion】 Bacillota, Bacteroidota, Actinomycetota and Verrucomicrobiota were the dominant flora in the feces of pure blood horses during lactation.Supplement of 15 mg/kg BW ellagic acid could improve the fecal flora diversity and increase the species of fecal flora.

Key words: ellagic acid; lactation; foals; microbial diversity

中图分类号:

S821.5

杨帆, 陈开旭, 郭翠洁, 臧长江, 贺林娇, 李晓斌. 补喂鞣花酸对哺乳中后期纯血马马驹粪便菌群多样性的影响[J]. 中国畜牧兽医, 2023, 50(11): 4482-4490.

YANG Fan, CHEN Kaixu, GUO Cuijie, ZANG Changjiang, HE Linjiao, LI Xiaobin. The Effect of Supplementing Ellagic Acid on the Diversity of Fecal Microbiota in Pure-blooded Foals in the Middle and Late Stages of Lactation[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(11): 4482-4490.

参考文献

[1] KLEIN-JÖBSTL D, SCHORNSTEINER E, MANN E, et al.Pyrosequencing reveals diverse fecal microbiota in simmental calves during early development[J].Frontiers in Microbiology, 2014, 5:622.
[2] 裴河欢, 张美凤, 覃洋琛, 等.多酚类化合物药理作用研究进展[J].中国药业, 2022, 31(23):124-127. PEI H H, ZHANG M F, QIN Y C, et al.Research progress on pharmacological effects of polyphenolic compounds[J].China Pharmaceuticals, 2022, 31(23):124-127.(in Chinese)
[3] 陈玫伶, 勾玲, 卢海啸.鞣花酸药理作用研究进展[J].世界科学技术-中医药现代化, 2023, 25(1):196-202. CHEN M L, GOU L, LU H X.The pharmacology research progress of ellagic acid[J].Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology, 2023, 25(1):196-202.(in Chinese)
[4] 谢凯桓, 丁鹏, 张云露, 等.单宁及其组分的生物活性及在畜禽生产中的应用[J].饲料研究, 2020, 43(4):130-133. XIE K H, DING P, ZHANG Y L, et al.Biological activity and application in livestock and poultry production of tannin and its components[J]. Feed Research, 2020, 43(4):130-133.(in Chinese)
[5] 赵铭伟.鞣花酸对断奶仔猪小肠黏膜形态及肠黏膜屏障功能的影响[D].南宁:广西大学, 2020. ZHAO M W.Effects of ellagic acid on the intestinal mucosa morphologyand intestinal mucosal barrier function of weaned piglets[D].Nanning:Guangxi University, 2020.(in Chinese)
[6] SHANDILYA S, KUMAR S, JHA N K, et al.Interplay of gut microbiota and oxidative stress:perspective on neurodegeneration and neuroprotection[J].Journal of Advanced Research, 2022, 38:223-244.
[7] 张盼望, 杨长伟, 吉素娜, 等.肠道微生物与其相关疾病[J].世界华人消化杂志, 2016, 24(15):2355-2360. ZHANG P W, YANG C W, JI S N, et al.Gut microbiota and related diseases[J].World Chinese Journal of Digestology, 2016, 24(15):2355-2360. (in Chinese)
[8] 张新雨, 李胜利.西藏地区荷斯坦犊牛断奶前后粪便菌群差异分析[J].动物营养学报, 2022, 34(9):5800-5808. ZHANG X Y, LI S L.Analysis of fecal bacteria community difference between pre-weaning and post-weaning calves in Tibet region[J].Chinese Journal of Animal Nutrition, 2022, 34(9):5800-5808.(in Chinese)
[9] 李晓斌.3~6月龄伊犁马肠道菌群多样性及补喂益生菌调控作用的研究[D].乌鲁木齐:新疆农业大学, 2017. LI X B.Study on intestinal flora diversity and regulation of supplement feeding probiotics of 3-6-month-old Yili horse[D].Urumqi:Xinjiang Agricultural University, 2017. (in Chinese)
[10] 李倩, 黄新新, 张文杰, 等.不同谷物淀粉对断奶马驹盲肠菌群多样性的影响[J].动物营养学报, 2022, 34(1):488-499. LI Q, HUANG X X, ZHANG W J, et al.Effects of different cereal ctarchees on cecal microflora diversity of weaned foals[J].Chinese Journal of Animal Nutrition, 2022, 34(1):488-499.(in Chinese)
[11] 杨帆, 李海英, 陈贤婷, 等.日粮中添加微生物制剂对哺乳期马驹生长性能、血浆激素水平和免疫指标的影响[J].中国饲料, 2015, 17:20-24. YANG F, LI H Y, CHEN X T, et al.The effect of adding microbial preparations to the diet on the growth performance, plasma hormone levels, and immune indicators of lactating foals[J].China Feed, 2015, 17:20-24.(in Chinese)
[12] 邓宇, 杨诗颖, 冼文妍, 等.鞣花酸在UM-A型人肠道菌群中体外转化及对肠道菌群的影响[J].食品与发酵工业, 2023, 49(2):34-40. DENG Y, YANG S Y, XIAN W Y, et al.Biotransformation of ellagic acid by gut microbiota from urolithin metabotype A and its effect on gut microbiota in vitro[J].Food and Fermentation Industries, 2023, 49(2):34-40.(in Chinese)
[13] 黄新新, 朱静, 李璇玥, 等.灌喂石榴皮瓤提取物对小鼠盲肠菌群结构及多样性的影响[J].饲料工业, 2021, 42(3):25-31. HUANG X X, ZHU J, LI X Y, et al.Effects of pomegranate skin extract on the structure and diversity of mouse cecum microbiota[J].Feed Industry, 2021, 42(3):25-31.(in Chinese)
[14] SELMA M, ESPIN J C, TOMAS-BARBERAN F A.Interaction between phenolics and gut microbiota:Role in human health[J].Journal of Agricultural and Food Chemistry, 2009, 57(15):6485-6501.
[15] 李晓斌, 李海, 臧长江, 等.断奶前马驹与母马粪便菌群多样性分析[J].中国畜牧兽医, 2020, 47(4):1050-1057. LI X B, LI H, ZANG C J, et al.Diversity analysis of fecal flora on pre-weaning foal and mare[J].China Animal Husbandry & Veterinary Medicine, 2020, 47(4):1050-1057.(in Chinese)
[16] LI X B, HUANG X X, LI Q, et al.Effects of different grains on bacterial diversity and enzyme activity associated with digestion of starch in the foal stomach[J].BMC Veterinary Research, 2022, 18(1):1-17.
[17] GONZÁLEZ-SARRÍAS A, VILLALBA R, ROMO-VAQUERO M, et al.Clustering according to urolithin metabotype explains the interindividual variability in the improvement of cardiovascular risk biomarkers in overweight-obese individuals consuming pomegranate:A randomised clinical trial[J].Molecular Nutrition & Food Research, 2017, 61(5):1600830.
[18] LARROSA M, GONZÁLEZ-SARRÍAS A, YÁÑEZ-GASCÓN M J, et al.Anti-inflammatory properties of a pomegranate extract and its metabolite urolithin-A in a colitis rat model and the effect of colon inflammation on phenolic metabolism[J].The Journal of Nutritional Biochemistry, 2010, 21(8):717-725.
[19] TOMÁS-BARBERÁN F A, GONZÁLEZ-SARRÍAS A, GARCÍA-VILLALBA R, et al.Urolithins, the rescue of "old" metabolites to understand a "new" concept:Metabotypes as a nexus among phenolic metabolism, microbiota dysbiosis, and host health status[J].Molecular Nutrition and Food Research, 2017, 61(1):1-35.
[20] 张聪.鞣花酸改善小鼠肝脏脂肪变性的作用机制及尿石素A脂质体制备研究[D].武汉:湖北中医药大学, 2020. ZHANG C.Study on the mechanism of ellagic acid ameliorates hepatic steatosis in mice and the preparation of urolithin A liposomes[D].Wuhan:Hubei University of Traditional Chinese Medicine, 2020.(in Chinese)
[21] YOUNG V B.The intestinal microbiota in health and disease[J].Current Opinion in Gastroenterology, 2012, 28(1):63-69.