禽类产品及饲料中尼卡巴嗪药物检测分析方法研究进展

doi:10.16431/j.cnki.1671-7236.2021.12.041

摘要/Abstract

摘要： 尼卡巴嗪(nicarbazin,NIC)是一类由4,4’-二硝基苯脲(4,4-dinitrocarbanilide,DNC)和2-羟基-4,6-二甲基嘧啶(2-hydroxyl-4,6-dimethylpyrimidine,HDP)组成的等分子复合物,其中DNC为其残留标志物。NIC因安全高效被广泛应用于鸡和火鸡等禽类球虫病防治。近年来,NIC残留问题高发,严重威胁动物源性食品安全和消费者生命健康安全。目前,针对禽类产品和饲料中NIC分析方法主要包括仪器分析方法和免疫分析方法两大类。其中,仪器分析方法作为确证方法,具有高特异性、高灵敏度、结果可靠的优点,主要包括高效液相色谱法(high performance liquid chromatography,HPLC)和液相色谱-串联质谱法(liquid chromatography-tandem mass spectrometry,LC-MS/MS)等。免疫分析方法是NIC快速检测方法的主流技术,具有速度快、成本低、实用性强的优点,主要包括酶联免疫检测法(enzyme-linked immunosorbent assay,ELISA)、免疫层析方法(immunochromatography,IC)、表面等离子体共振(surface plasmon resonance,SPR)、荧光免疫检测法(fluorescent immunoassay,FIA)和流式细胞术(flow cytometry,FCM)等。笔者综述了近40年来国内外禽类产品和饲料中NIC的检测分析方法,以期为NIC残留监控提供理论和实践指导依据。

关键词: 尼卡巴嗪; 检测分析; 禽类产品; 饲料; 仪器分析法; 免疫分析法

Abstract: Nicarbazin (NIC) is a class of compounds composed of 4 4’-dinitrocarbanilide (DNC) and 2-hydroxyl-4, 6-dimethylpyrimidine (HDP), in which DNC is its residual marker.NIC is widely used in coccidiosis control of chickens and turkeys due to its safety and high efficiency.In recent years, its residue problem was high, which seriously threatened the safety of animal derived food and the safety of consumers’ life and health.At present, the analysis methods for NIC in poultry products and feed mainly include instrumental analysis methods and immunoassay methods.Among them, instrumental analysis methods, as confirmatory methods, have the advantages of high specificity, high sensitivity and reliable results, mainly including high performance liquid chromatography (HPLC) and liquid chromatography tandem mass spectrometry (LC-MS/MS).Immunoassay is the mainstream technology for rapid detection of NIC, which has the advantages of high inspection speed, low cost and strong practicability.It mainly includes enzyme-linked immunosorbent assay (ELISA), immunochromatography (IC), surface plasmon resonance (SPR), fluorescent immunoassay (FIA) and flow cytometry (FCM) etc.This paper summarized the detection and analysis methods of NIC in poultry products and feed at home and abroad in recent 40 years, so as to provide theoretical and practical guidance for NIC residue monitoring.

Key words: nicarbazine; detection and analysis; poultry products; feed; instrumental analysis methods; immunoassays

中图分类号:

S859

尹永康, 王晓亮, 崔艳丽, 梁晓. 禽类产品及饲料中尼卡巴嗪药物检测分析方法研究进展[J]. 中国畜牧兽医, 2021, 48(12): 4725-4734.

YIN Yongkang, WANG Xiaoliang, CUI Yanli, LIANG Xiao. Research Progress on the Detection and Analysis Methods of Nicarbazine in Poultry Products and Feed[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4725-4734.

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