国内部分省市肉兔及蜱虫携带土拉杆菌的流行病学调查

doi:10.16431/j.cnki.1671-7236.2020.12.031

中国畜牧兽医 ›› 2020, Vol. 47 ›› Issue (12): 4069-4075.doi: 10.16431/j.cnki.1671-7236.2020.12.031

国内部分省市肉兔及蜱虫携带土拉杆菌的流行病学调查

李书光^1,2,3, 王艺晖², 曲光刚^1,2,3, 孙翠平⁴, 杨丽芳⁴, 林初文¹, 沈志强^1,4, 何诚^1,2

1. 山东省滨州畜牧兽医研究院, 滨州 256600;
2. 中国农业大学动物医学院, 北京 100193;
3. 山东省滨州畜禽蜂胶疫苗研究开发推广中心滨州 256600;
4. 山东绿都生物科技有限公司, 滨州 256600

收稿日期:2020-05-07 出版日期:2020-12-20 发布日期:2020-12-18
通讯作者: 何诚 E-mail:hecheng@cau.edu.cn
作者简介:李书光(1982-),男,山东惠民人,博士,研究方向:分子细菌学研究和生物制品学,E-mail:lshug@163.com
基金资助:
国家重点研发计划中日政府间合作项目（2016YFE0127100）；泰山学者项目（TS201511084）

Epidemiological Investigation of Francisella tularensis Transmitted by Rabbits and Ticks in Some Provinces of China

LI Shuguang^1,2,3, WANG Yihui², QU Guanggang^1,2,3, SUN Cuiping⁴, YANG Lifang⁴, LIN Chuwen¹, SHEN Zhiqiang^1,4, HE Cheng^1,2

1. Shandong Binzhou Animal Science and Veterinary Medicine Academy, Binzhou 256600, China;
2. College of Veterinary Medicine, China Agricultural University, Beijing 100193, China;
3. Shandong Binzhou Research, Development and Promotion Center for Livestock and Poultry Propolis Vaccine, Binzhou 256600, China;
4. Shandong Lvdu Biotechnology Co., Ltd., Binzhou 256600, China

Received:2020-05-07 Online:2020-12-20 Published:2020-12-18

摘要/Abstract

摘要： 本调查旨在探明土拉杆菌在国内部分省份的流行情况。利用本实验室建立的土拉杆菌通用及亚种PCR检测研究方法，对国内肉兔饲养密度比较大的省份和牛羊存栏的部分省市开展了肉兔和蜱虫中土拉杆菌的检测，并对阳性样本进行了土拉杆菌亚种的鉴定和基因测序验证。研究结果表明，肉兔肝脏组织和牛羊携带蜱虫中土拉杆菌DNA检测阳性，其中218份肉兔样本中12份阳性（阳性率5.5%），490份蜱虫样本中15份阳性（阳性率3.1%）；从地区分布来看，山东、河南、四川肉兔病料检测阳性，云南、山东蜱虫阳性率高；PCR检测显示其亚种为F.h，为土拉杆菌毒力较强的亚种。本次调查显示土拉杆菌在肉兔、蜱虫中存在，山东、云南地区存在土拉杆菌病公共卫生安全风险，应引起有关科研院校、医疗单位和政府部门的高度重视。

关键词: 土拉杆菌; 肉兔; 蜱虫; 流行病学调查

Abstract: The aim of this investigation was to find out the prevalence of Francesella tularensis in some provinces of China. Using the general and subspecies PCR detection method for Francesella tularensis established in our laboratory, PCR detection of Francesella tularensis in rabbits and ticks was carried out in provinces with high-density rabbits feeding and some provinces and cities with sheep and cattle stock. The results showed that DNA detection of Francesella tularensis from rabbit tissues and ticks carried by cattle and sheep was positive. 12 out of 218 rabbit samples were positive (5.5% positive rate), and 15 tick samples were positive (3.1%) of 490 tick samples. In terms of geographical distribution, most of the positive rabbit samples were from Shandong, Henan and Sichuan province, however, ticks collected from Yunnan and Shandong province showed a higher positive rate. PCR detection showed that Francesella tularensis subspecies in this investigation was F.h, which was a subspecies with strong toxicity. This investigation revealed that Francesella tularensis presented in rabbits and ticks, public health and safety risks of Francesella tularensis existed in Shandong and Yunnan province, which should be paid more attention by the relevant scientific research institutions, medical institutions and government departments.

Key words: Francesella tularensis; rabbits; ticks; epidemiological investigation

中图分类号:

李书光, 王艺晖, 曲光刚, 孙翠平, 杨丽芳, 林初文, 沈志强, 何诚. 国内部分省市肉兔及蜱虫携带土拉杆菌的流行病学调查[J]. 中国畜牧兽医, 2020, 47(12): 4069-4075.

LI Shuguang, WANG Yihui, QU Guanggang, SUN Cuiping, YANG Lifang, LIN Chuwen, SHEN Zhiqiang, HE Cheng. Epidemiological Investigation of Francisella tularensis Transmitted by Rabbits and Ticks in Some Provinces of China[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(12): 4069-4075.

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国内部分省市肉兔及蜱虫携带土拉杆菌的流行病学调查

Epidemiological Investigation of Francisella tularensis Transmitted by Rabbits and Ticks in Some Provinces of China

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