2018年广西重要猪源病毒性疫病流行病学调查

doi:10.16431/j.cnki.1671-7236.2020.01.021

摘要/Abstract

摘要： 为了解2018年广西猪群重要疫病流行情况，试验采集广西各地的病死猪组织样品及病猪腹泻拭子，应用多重实时荧光定量RT-PCR检测猪瘟病毒（CSFV）、猪繁殖与呼吸综合征病毒（PRRSV），应用多重实时荧光定量PCR检测猪伪狂犬病病毒（PRV）、猪圆环病毒1型（PCV1）、猪圆环病毒2型（PCV2）及猪圆环病毒3型（PCV3），应用多重RT-PCR检测猪流行性腹泻病毒（PEDV）、猪德尔塔冠状病毒（PDCoV）、猪传染性胃肠炎病毒（TGEV）和猪轮状病毒（PRoV）。结果显示，所检测的694份组织样品中，CSFV、PRRSV、HP-PRRSV、PRV、PCV1、PCV2、PCV3的阳性率分别为11.10%、18.88%、7.20%、5.19%、2.45%、67.00%和5.76%；2种病原混合感染率为41.21%，3种病原混合感染率为4.32%，其中PRRSV和PCV2混合感染率最高。所检测的792份肠内容物及拭子腹泻样品中，PEDV、PDCoV、TGEV、PRoV的阳性率分别为9.72%、5.81%、1.77%和6.31%；2种病原混合感染率为5.30%，其中PEDV和PRoV混合感染率最高。结果表明，当前多种重要病毒性疫病仍在广西猪群发生和流行，并且多重感染普遍存在，应进一步加强监测和防控。

关键词: 猪; 病毒性疫病; 流行病学调查; 监测

Abstract: To investigate the epidemiology of major porcine viral diseases in Guangxi during 2018,clinical tissue samples were detected by multiplex Real-time RT-PCR for classical swine fever virus (CSFV) and porcine reproductive and respiratory syndrome virus (PRRSV),multiplex Real-time PCR for porcine pseudorabies virus (PRV),porcine circovirus (PCV) type 1 (PCV1),PCV2 and PCV3.Clinical diarrhea samples were detected by multiplex RT-PCR for porcine epidemic diarrhea virus (PEDV),porcine deltacoronavirus (PDCoV),porcine transmissible gastroenteritis virus (TGEV) and porcine rotavirus (PRoV).The results showed that of 694 tissue samples detected,the positive rates of CSFV,PRRSV,HP-PRRSV,PRV,PCV1,PCV2,PCV3 were 11.10%,18.88%,7.20%,5.19%,2.45%,67.00% and 5.76%,respectively,of which co-infection rates of two pathogens and three pathogens were 41.21% and 4.32% respectively,and PRRSV and PCV2 co-infection rate was the highest.792 diarrhea samples (intestinal contents and rectal swabs) were detected,the positive rates of PEDV,PDCoV,TGEV and PRoV were 9.72%,5.81%,1.77% and 6.31%,respectively,of which co-infection rate of two pathogens was 5.30%,and PEDV and PRoV co-infection rate was the highest.The results indicated that several major viral diseases were still widespread distribution in swine herds from Guangxi,and mixed infections were common.It should be further imposed for epidemic surveillance,prevention and control of these diseases in the future.

Key words: swine; viral disease; epidemiological investigation; surveillance

中图分类号:

S858.28

施开创, 尹彦文, 王孝德, 谢守玉, 石永胜, 刘宏梅, 陆文俊, 屈素洁, 冯淑萍, 粟艳琼. 2018年广西重要猪源病毒性疫病流行病学调查[J]. 中国畜牧兽医, 2020, 47(1): 174-181.

SHI Kaichuang, YIN Yanwen, WANG Xiaode, XIE Shouyu, SHI Yongsheng, LIU Hongmei, LU Wenjun, QU Sujie, FENG Shuping, SU Yanqiong. Epidemiological Investigation of Major Porcine Viral Diseases in Guangxi During 2018[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(1): 174-181.

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